| 摘要 | 第1-8页 |
| Abstract | 第8-10页 |
| Abbreviations | 第10-12页 |
| Content | 第12-16页 |
| CHAPTER Ⅰ INTRODUCTION | 第16-32页 |
| ·Tomato(Solanum lycopersicum) | 第16-17页 |
| ·Tomato production | 第16-17页 |
| ·Tomato diseases | 第17页 |
| ·Phytophtora infestans | 第17-22页 |
| ·The pathogen | 第17-18页 |
| ·Disease cycle | 第18-19页 |
| ·Variability of isolates | 第19-20页 |
| ·Disease symptoms | 第20页 |
| ·Disease screening methods | 第20-21页 |
| ·Detached leaves assay | 第21页 |
| ·Whole plant assay | 第21-22页 |
| ·Field screening | 第22页 |
| ·Strategies for late blight management | 第22-25页 |
| ·Field management | 第23页 |
| ·Chemical control | 第23页 |
| ·Resistant genetic resources | 第23-24页 |
| ·Resistant genes | 第24页 |
| ·Mapping of quantitative resistance | 第24-25页 |
| ·Pyramiding R- genes for durable resistance in tomato breeding | 第25页 |
| ·Mechanism involved in tomato resistance against P. infestans | 第25-27页 |
| ·Roles of SA and JA/ET | 第26-27页 |
| ·Roles of JA and ET | 第27页 |
| ·Age-related resistance | 第27-29页 |
| ·General information on ARR | 第27-28页 |
| ·Possible mechanisms for ARR | 第28-29页 |
| ·Future prospective | 第29页 |
| ·Hypothesis of research work | 第29页 |
| ·Objectives of research work | 第29-30页 |
| ·Scope of thesis | 第30-32页 |
| CHAPTER Ⅱ Evaluation of the Phytophthora infestans resistance in a global collection of wild tomatospecies Solanum pimpinellifolium | 第32-44页 |
| ·Introduction | 第32-33页 |
| ·Materials and Methods | 第33-36页 |
| ·Plant materials and growth conditions | 第33页 |
| ·Inoculums preparation | 第33-34页 |
| ·Pathogenicity test of isolates | 第34页 |
| ·Disease scoring | 第34页 |
| ·DNA extraction and PCR | 第34页 |
| ·Data analysis | 第34-36页 |
| ·Results | 第36-42页 |
| ·Physiological races of P. infestans isolate | 第36-37页 |
| ·Evaluation for resistance in wild germplasm | 第37-38页 |
| ·Geographical distribution and genetic variation | 第38-42页 |
| ·Discussion | 第42-44页 |
| ·The essential importance for characterization of tomato P. infeslans isolates in China | 第42页 |
| ·Evaluation of a S. pimpinellifolium global collection against late blight disease | 第42-43页 |
| ·Co-evolution between S. pimpinellifolium and P. infeslans needs to be addressed | 第43-44页 |
| CAHPTER Ⅲ Resistance mediated by R-gene and QTLs exhibited age-related resistance againstPhytophthora infestans in tomato | 第44-56页 |
| ·Introduction | 第44-45页 |
| ·Materials and Methods | 第45-47页 |
| ·Plant materials and growth conditions | 第45-46页 |
| ·Inoculum preparation | 第46页 |
| ·Whole-plant assay | 第46页 |
| ·Disease scoring | 第46页 |
| ·Histological analysis | 第46-47页 |
| ·Statistical analysis | 第47页 |
| ·Results | 第47-53页 |
| ·Age-related resistance in Ph-3 gene against P. infestans | 第47页 |
| ·Age-related resistance in QTLs against P. infestans | 第47-50页 |
| ·Nature of interaction between Ph-3 and P. infestans at different time points | 第50-51页 |
| ·Induction of HR cells in plants harboring Ph-3 gene | 第51-53页 |
| ·Discussion | 第53-56页 |
| ·A common phenomenon for ARR against P. infestans in tomato | 第53-54页 |
| ·Histological analysis of Ph-3 based resistance against P. infestans in tomato | 第54-56页 |
| CHAPTER Ⅳ Role of phytohormone pathways in Ph-3 based resistance against Phytophthora infestansin tomato | 第56-70页 |
| ·Introduction | 第56-57页 |
| ·Materials and Methods | 第57-60页 |
| ·Plant materials and growth conditions | 第57-58页 |
| ·Selection of homozygous plants for Ph-3 gene | 第58页 |
| ·Inoculum preparation | 第58页 |
| ·Disease scoring | 第58页 |
| ·Marker genes for phytohormone pathways | 第58页 |
| ·RNA isolation and cDNA synthesis | 第58页 |
| ·RT-PCR | 第58-59页 |
| ·Virus-induced gene silencing (VIGS) | 第59页 |
| ·Disease scoring for silenced plants | 第59页 |
| ·Statistical analysis | 第59-60页 |
| ·Results | 第60-67页 |
| ·The expression of marker genes for phytohormone pathways in Ph-3 based resistance to P. infestans | 第60-61页 |
| ·Resistance in Ph-3 gene influenced by pathway mutants | 第61页 |
| ·Effect of SA deficiency on Ph-3 gene in tomato | 第61页 |
| ·ET is required for Ph-3 resistance in tomato against P. infestans | 第61-62页 |
| ·JA deficiency has no effect on Ph-3 resistance | 第62-64页 |
| ·RT-PCR analysis for SA, ET, and JA pathways in Ph-3 resistance to P. infeslans at different age | 第64-66页 |
| ·Silencing of CTR1 gene in Ph-3 by VIGS | 第66-67页 |
| ·Discussion | 第67-70页 |
| ·SA pathway in Ph-3 mediated resistance against P. infestans in tomato | 第67页 |
| ·Role of ET pathway in Ph-3 mediated resistance against P. infestans in tomato | 第67-68页 |
| ·Ph-3 gene resistance in tomato is independent of JA pathway | 第68-70页 |
| CONCLUSIONS | 第70-73页 |
| REFERENCES | 第73-87页 |
| ACKNOWLEDGEMENTS | 第87-88页 |
| CURRICULAM VITAE | 第88页 |
