| 摘要 | 第1-7页 |
| Abstract | 第7-14页 |
| CHAPTER Ⅰ INTRODUCTION | 第14-42页 |
| ·HIGH TEMPERATURE STRESS ON GROWTH AND DEVELOPMENT OF RICE PLANT | 第15-16页 |
| ·HIGH TEMPERATURE STRESS ON VEGETATIVE PHASE | 第16-17页 |
| ·HIGH TEMPERATURE STRESS ON REPRODUCTIVE PHASE | 第17-20页 |
| ·HIGH TEMPERATURE STRESS ON GRAIN FILLING PHASE | 第20-23页 |
| ·INTERACTIVE EFFECT OF HIGH TEMPERATURE WITH OTHER CLIMATIC FACTORS | 第23-25页 |
| ·ROle of humidity in spikelet sterility | 第23页 |
| ·Atmospheric CO_2 level | 第23-24页 |
| ·Endogenous hormonal level | 第24-25页 |
| ·Wind speed at flowering | 第25页 |
| ·TRAITS RELATED TO TOLERANCE OF HIGH TEMPERATURE STRESS | 第25-28页 |
| ·Plant architecture | 第25页 |
| ·Time of flowering and anthesis | 第25-27页 |
| ·Length of anther | 第27页 |
| ·Size of basal pore | 第27页 |
| ·Length of basal dehiscence of anther | 第27-28页 |
| ·PHYSIOLOGICAL AND BIOCHEMICAL MECHANISMS OF HEAT TOLERANCE | 第28-31页 |
| ·Role of HSPs in inducing thermo tolerance | 第29-30页 |
| ·Factors other than HSPs contributing to thermo-tolerance | 第30-31页 |
| ·GENETICS OF HEAT TOLERANCE | 第31-40页 |
| ·Heat tolerance is a quantitative trait | 第31-32页 |
| ·QTLs analysis on heat stress tolerant | 第32-37页 |
| ·Proteomic approach and heat responsive proteins in rice | 第37-38页 |
| ·HSPs and other protection proteins | 第38页 |
| ·Heat tolerant gene cloning and transferring | 第38-39页 |
| ·Transcriptome study on heat tolerance rice | 第39-40页 |
| ·OBJECTIVES OF THE RESEARCHh WORK | 第40-42页 |
| CHAPTER Ⅱ MATERIALS AND METHODS | 第42-53页 |
| ·MATERIALS AND METHODS | 第43-46页 |
| ·Development of plant materials | 第43-44页 |
| ·Preliminary screening of the BC_2F_2 bulk populations for heat tolerant (HT) at the reproductive stage | 第44-45页 |
| ·Confirmation of the selected BC_2F_4 lines for HT at the reproductive stage | 第45页 |
| ·Second round evaluation for HT ILs population | 第45-46页 |
| ·DNA EXTRACTION AND GENOTYPING WITH MOLECULAR MARKERS | 第46页 |
| ·PREPARE CTAB (CELYTRIMETHYL AMMONIUM BROMIDE) BUFFER AND TE BUFFER | 第46-47页 |
| ·PREPARING LEAF SAMPLES | 第47-48页 |
| ·SSR TECHNOLOGY | 第48-52页 |
| ·PCR Reaction | 第49页 |
| ·PCR mix for one reaction | 第49-50页 |
| ·Temperature Profile | 第50-52页 |
| ·Electrophoresis Using Polyacrylamide Gel | 第52页 |
| ·DATA ANALYSIS | 第52-53页 |
| CHAPTER Ⅲ HEAT TOLERANCE PERFORMANCES OF INTROGRESSION LINES DERIVED WITHSH527 AND MH86 BACKGROUNDS | 第53-73页 |
| ·SELECTION EFFICIENCY FOE HT AND GENETIC DIVERSITY IN THE SELECTED ILS | 第53页 |
| ·PERFORMANCES OF THE 13 POPULATIONS UNDER HEAT STRESS AND NORMAL CONDITIONS | 第53-60页 |
| ·Extreme lines and selection efficiency for two backgrounds | 第58页 |
| ·Average trait value of 9 populations change from mean traits of SH527 (RP) | 第58页 |
| ·Average trait value of 4 populations change from mean traits of MH86 (RP) | 第58-60页 |
| ·Promising lines developed | 第60页 |
| ·TEMPERATURE AND RELATIVE HUMIDITY COMBINE EFFECT FOR SPIKELET FERTILITY | 第60-63页 |
| ·DEVELOPMENT OF ILS WITH SIGNIFICANTLY IMPROVED HT | 第63页 |
| ·THE GENOME-WIDE RESPONSES TO SELECTION FOR HT | 第63-64页 |
| ·PHENOTYPIC ANALYSIS OF TWO POPULATIONS IN HUNAN AND HANGZHOU | 第64-69页 |
| ·PROMISING LINES DEVELOPED | 第69-73页 |
| CHAPTER Ⅳ DETECTION OF QTLS IN RICE TRAIT RELATED TO HEAT TOLERANT USINGSELECTED INTROGRESSION LINES OF SH527 | 第73-82页 |
| ·DEVELOPMENT OF EXPERIMENTAL POPULATION | 第73页 |
| ·QTL DETECTION AND ANALYSIS | 第73-79页 |
| ·Chi-square test for finding linkage markers | 第73页 |
| ·One-way ANOVA | 第73-79页 |
| ·DISCUSSION | 第79-81页 |
| ·CONCLUSION | 第81-82页 |
| CHAPTER Ⅴ QTLS MAPPING FOR HEAT AND YIELD RELATED TRAITS WITH MINGHUI86BACKGROUND | 第82-102页 |
| ·DEVELOPMENT OF MAPPING POPULATIONS WITH BACKCROSS | 第82页 |
| ·DNA EXTRACTION AND SSR GENOTYPING | 第82页 |
| ·DEVELOPMENT OF LINKAGE MAP AND QTL ANALYSIS | 第82-101页 |
| ·QTL mapping of heading date | 第82-83页 |
| ·Mapping QTLs for Plant height | 第83页 |
| ·Mapping QTLs for panicle number | 第83-84页 |
| ·Mapping QTLs for filled grain number per panicle | 第84-85页 |
| ·Mapping QTLs for spikelet number per panicle | 第85页 |
| ·Mapping QTLs for filled grain rate | 第85-86页 |
| ·Mapping QTLs for thousand grain weight | 第86页 |
| ·Mapping QTLs for grain yield per plant | 第86-87页 |
| ·Mapping QTLs for heat tolerant index | 第87-101页 |
| ·DISCUSSION | 第101-102页 |
| CHAPTER Ⅵ CONCLUSION | 第102-107页 |
| ·PHENOTYPIC PERFORMANCES OF NINE POPULATIONS WITH SHUHUI527 BACKGROUND | 第102页 |
| ·PHENOTYPIC PERFORMANCES OF FOUR POPULATIONS WITH MINGHUI86 BACKGROUND | 第102-103页 |
| ·IDENTIFICATION OF QTLs RELATED TO HEAT TOLERANCE USING SH527 BACKGROUND | 第103页 |
| ·IDENTIFICATION OF QTLs RELATED to HEAT TOLERANCE USING MH86 BACKGROUND | 第103页 |
| ·POSSIBLE EXPERIMENTAL ERROR UNDER SHED CONDITION | 第103-105页 |
| ·High Relative Humidity,(RH) | 第103-104页 |
| ·Reductions of incoming radiation and its properties | 第104-105页 |
| ·Other abiotic and biotic factors | 第105页 |
| ·ELEVATION OF TEMPERATURE AROUND THE CROP CANOPY WITHOUP USING PLASTIC SHED | 第105页 |
| ·FUTURE BREEDING FOR HT BASED ON PRESENT KNOWLEDGE | 第105-107页 |
| List of references | 第107-119页 |
| Acknowledgement | 第119-120页 |
| CURRICULUM VITAE | 第120页 |
