| Abstract | 第7-8页 |
| 摘要 | 第9-10页 |
| List of abbreviations | 第10-18页 |
| 1. Introduction | 第18-37页 |
| 1.1 Assisted Reproductive Technology | 第19-20页 |
| 1.2 Ovarian stimulation | 第20-21页 |
| 1.3 History of ovarian stimulation | 第21-22页 |
| 1.4 Medications used for ovarian stimulation | 第22-23页 |
| 1.5 Complications of ovarian stimulation | 第23-25页 |
| 1.6 Effect of ovarian stimulation on oocyte and embryo developmentalcompetence | 第25-26页 |
| 1.7 Epigenetic effect of ovarian stimulation on oocytes and embryos | 第26-28页 |
| 1.8 Effect of ovarian stimulation on uterine receptivity | 第28-30页 |
| 1.9 Epigenetic effect of ovarian stimulation on uterine receptivity | 第30-32页 |
| 1.10 Physiology of implantation | 第32-33页 |
| 1.11 Stages of implantation | 第33-36页 |
| 1.12 Objectives of the study | 第36-37页 |
| 2. Materials and Methods | 第37-53页 |
| 2.1 Biological, chemicals, kits, and other materials | 第37-39页 |
| 2.2 Animals | 第39页 |
| 2.3 Ovarian stimulation and collection of uterine samples | 第39页 |
| 2.4 Genetic effects of ovarian stimulation on uterine receptivity | 第39-46页 |
| 2.4.1 RNA extraction | 第39-43页 |
| 2.4.2 Microarray hybridization and data processing | 第43页 |
| 2.4.3 Functional annotation of DEGs | 第43-44页 |
| 2.4.4 c DNA preparation | 第44页 |
| 2.4.5 Quantitative RT-PCR | 第44-46页 |
| 2.5 Epigenetic effects of ovarian stimulation on uterine receptivity | 第46-52页 |
| 2.5.1 Genomic DNA extraction | 第46-49页 |
| 2.5.2 Methyl-DNA Immuoprecipitation | 第49页 |
| 2.5.3 Data analysis | 第49-50页 |
| 2.5.4 Functional Annotation of differentially methylated genes | 第50页 |
| 2.5.5 Bisulfite pyrosequencing methylation analysis | 第50-52页 |
| 2.6 Uterine histology | 第52页 |
| 2.7 Statistical analysis | 第52-53页 |
| 3. Results | 第53-83页 |
| 3.1 Genetic effects of ovarian stimulation on uterine receptivity | 第53-66页 |
| 3.1.1 Gene expression differences between 5 iu stimulated uteri and unstimulatedcontrols | 第56-62页 |
| 3.1.2 Gene expression differences between 10 iu stimulated uteri andunstimulated controls | 第62页 |
| 3.1.3 Gene expression differences between 10 iu and 5 iu stimulated groups | 第62-64页 |
| 3.1.4 Quantitative real time PCR | 第64-66页 |
| 3.2 Epigenetic effects of ovarian stimulation on uterine receptivity | 第66-80页 |
| 3.2.1 Promoter Methylation Profiling in 5 iu compared to unstimulated controls | 第71-75页 |
| 3.2.2 Promoter methylation profiling in 10 iu compared to unstimulated controls | 第75-78页 |
| 3.2.3 Promoter methylation profiling in 10 iu compared to 5 iu stimulated group | 第78-79页 |
| 3.2.4 Pyrosequencing methylation analysis | 第79-80页 |
| 3.3 Uterine histology | 第80-83页 |
| 4. Discussion | 第83-92页 |
| 4.1 Genetic effects of ovarian stimulation on uterine receptivity | 第83-87页 |
| 4.2 Epigenetic effects of ovarian stimulation on uterine receptivity | 第87-90页 |
| 4.3 The key histological features of the endometrium following ovarian stimulation | 第90-92页 |
| 5. Conclusions | 第92-93页 |
| 6. References | 第93-116页 |
| 7. Acknowledgement | 第116-118页 |
| 8. List of published papers | 第118-120页 |
