首页--农业科学论文--农作物论文--经济作物论文--棉论文

陆地棉纤维品质性状QTL鉴定与qFS21精细定位

摘要第8-10页
Abstract第10-11页
Chapter one Introduction第12-28页
    1.1 Classification of Gossypium第12-13页
    1.2 Cotton fiber development第13-15页
        1.2.1 Fiber initiation第13-14页
        1.2.2 Fiber elongation第14页
        1.2.3 Fiber secondary wall thickening第14-15页
        1.2.4 Fiber dehydration and maturity第15页
    1.3 Genes controlling the cotton fiber development第15-18页
        1.3.1 Fiber initiation related genes第15-16页
        1.3.2 Fiber elongation related genes第16-18页
        1.3.3 Fiber secondary wall thickening related genes第18页
        1.3.4 Fiber dehydration and maturation related genes第18页
    1.4 Cotton genomics第18-20页
    1.5 Recent advances in cotton genetic map construction第20-23页
        1.5.1 Interspecific genetic map of cotton第20-21页
        1.5.2 Intraspecific genetic map of Upland Cotton第21-23页
    1.6 Progress in mapping of important agronomic traits in cotton第23-28页
        1.6.1 Preliminary QTL mapping for cotton fiber quality第23-25页
        1.6.2 Advances in fine-mapping of important agronomic traits of cotton第25-28页
Chapter Two Whole Genome QTL detection for fibre quality traits第28-62页
    2.1 Introduction第28-30页
    2.2 Materials and Methods第30-37页
        2.2.1 Mapping Population第30页
        2.2.2 Extraction and purification of total genomic DNA第30-32页
        2.2.3 Genotyping of SSR primers第32-35页
        2.2.4 SLAF library construction and high-throughput sequencing第35页
        2.2.5 Sequencing data grouping and genotyping第35页
        2.2.6 Linkage map construction第35-36页
        2.2.7 Phenotypic data analysis第36页
        2.2.8 Preliminary QTL detection for fiber quality第36-37页
    2.3 Results and analysis第37-56页
        2.3.1 Phenotypic analysis of fiber quality traits第37-39页
        2.3.2 Analysis of SLAF-seq data and SLAF markers第39页
        2.3.3 Construction of the genetic map第39-40页
        2.3.4 Segregation distortion第40-41页
        2.3.5 Collinearity between the genetic and the physical map第41-49页
        2.3.6 QTL mapping第49-56页
    2.4 Discussion第56-60页
        2.4.1 Prominent features of SLAF-seq第56页
        2.4.2 SLAF genetic map第56-57页
        2.4.3 Segregation Distortion第57页
        2.4.4 QTL cluster第57-58页
        2.4.5 Stable and common QTL第58-60页
    2.5 Conclusion第60-62页
Chapter Three fine-mapping qFS21第62-84页
    3.1 Introduction第62-64页
    3.2 Materials and methods第64-67页
        3.2.1 Plant Material第64页
        3.2.2 Total genomic DNA extraction from genome第64页
        3.2.3 Genotyping第64页
        3.2.4 Construction of genetic map and QTL mapping第64-65页
        3.2.5 RNA Extraction第65-66页
        3.2.6 Parental expression profiling第66-67页
    3.3 Results and analysis第67-78页
        3.3.1 Fiber quality analysis第67-70页
        3.3.2 Construction of high density genetic map in F2 Population第70-73页
        3.3.3 QTL Mapping for fiber quality traits in F2 population第73-74页
        3.3.4 Fine-mapping of Fiber quality QTL in F2:3 population第74-75页
        3.3.5 Identification of candidate genes第75-78页
    3.4 Discussion第78-81页
        3.4.1 Fine-mapping population第78-79页
        3.4.2 Pleiotropism or linkage for QTL affecting multiple fiber traits第79-80页
        3.4.3 Candidate gene identification strategy第80-81页
    3.5 Conclusion第81-84页
        3.5.1 Construction of high density genetic map第81页
        3.5.2 QTL fine-mapping第81页
        3.5.3 Identification of candidate genes第81-84页
References第84-104页
Appendix第104-106页
Acknowledgement第106-108页
Publications第108页

论文共108页,点击 下载论文
上一篇:基于条目反应理论SEM的FGIDs证素辨证诊断模型的方法学研究
下一篇:烟草根际抑病土壤有益微生物的组学特征及对青枯病的拮抗作用研究