| 摘要 | 第9-12页 |
| Abstract | 第12-14页 |
| List of Abbreviations | 第15-16页 |
| 1 Background | 第16-19页 |
| 2 Literature Review | 第19-43页 |
| 2.1 Concept of microbe-mediated pathogenicity | 第19-21页 |
| 2.2 C.elegans as model host for studying bacterial virulence | 第21-22页 |
| 2.3 Factors affecting pathogenicity during bioassays | 第22-24页 |
| 2.4 Mechanisms of pathogenicity | 第24-26页 |
| 2.5 Virulence factors of Gram-negative bacteria | 第26-27页 |
| 2.6 Pathogenicity of different genera of Gram-negative bacteria against C. elegans | 第27-36页 |
| 2.6.1 Genus Pseudomonas | 第27-31页 |
| 2.6.2 Genus Salmonella | 第31-32页 |
| 2.6.3 Genus Burkholderia | 第32-36页 |
| 2.7 Response of C. elegans to bacterial infection | 第36-38页 |
| 2.8 Comparative genomics and its contributions in the virulence determination | 第38-39页 |
| 2.9 Patho-genomics of P. syringae and its virulence factors | 第39-43页 |
| 3 Materials and Methods | 第43-54页 |
| 3.1 Bacterial and C. elegans strains, media, plasmids, and culture conditions | 第43-45页 |
| 3.2 Maintenance and growth of C. elegans | 第45-46页 |
| 3.3 Synchronization of Caenorhabditis elegans | 第46-47页 |
| 3.4 Routine manipulation techniques of molecular biology | 第47-54页 |
| 3.4.1 Plasmid DNA extraction | 第48-49页 |
| 3.4.2 PCR and gel electrophoresis | 第49-50页 |
| 3.4.3 Preparation of Escherichia coli competent cells | 第50页 |
| 3.4.4 Plasmid transformation | 第50-51页 |
| 3.4.5 Total RNA extraction | 第51-52页 |
| 3.4.6 cDNA synthesis and RT-qPCR | 第52-53页 |
| 3.4.7 Preparation of competent cells of P. syringae and electroporation | 第53-54页 |
| 4 Comparative genomics of P. syringae MB03 and determination of its virulence genes | 第54-95页 |
| 4.1 Introduction | 第54-55页 |
| 4.2 Materials and Methods | 第55-58页 |
| 4.2.1 Genome sequencing | 第55页 |
| 4.2.2 Phylogenetic analysis | 第55-56页 |
| 4.2.3 Comparative genomics | 第56页 |
| 4.2.4 Patho-genomic analysis | 第56-58页 |
| 4.3 Results and Discussion | 第58-93页 |
| 4.3.1 General features of P. syringae MB03 genome | 第58-65页 |
| 4.3.2 Amino acid identity matrix and phylogenetic analysis | 第65-68页 |
| 4.3.3 Core and dispensable genome | 第68-72页 |
| 4.3.4 Genomic and pathogenicity islands | 第72-79页 |
| 4.3.5 Type Ⅲ secretion system effector identification and distribution | 第79-80页 |
| 4.3.6 Type Ⅵ secretion system | 第80-83页 |
| 4.3.7 Miscellaneous virulence factors and resistance against harsh conditions | 第83-85页 |
| 4.3.8 Homologs of nematicidal genes of P. aeruginosa | 第85-93页 |
| 4.4 Summary | 第93-95页 |
| 5 Pathogenicity of P. syringae MB03 against C. elegans | 第95-134页 |
| 5.1 Introduction | 第95-97页 |
| 5.2 Materials and Methods | 第97-110页 |
| 5.2.1 Bioassays | 第97-98页 |
| 5.2.2 Real time quantitative polymerase chain reaction (RT-qPCR) | 第98-103页 |
| 5.2.3 Knock out of potential nematicidal genes | 第103-109页 |
| 5.2.4 Gut colonization | 第109-110页 |
| 5.3 Results and Discussion | 第110-133页 |
| 5.3.1 Host-pathogen interaction varied from nonpathogenic to pathogenic withdifferent media | 第110-115页 |
| 5.3.2 Transcriptional response of potential nematicidal genes | 第115-120页 |
| 5.3.3 Role of phoB, phoP, clpA,kdpD and kdpE genes in pathogenicity of P.syringae MB03 | 第120-129页 |
| 5.3.4 P. syringae MB03 colonizes the gut of C. elegans | 第129-133页 |
| 5.4 Summary | 第133-134页 |
| 6 Concluding remarks and future prospects | 第134-136页 |
| 7 References | 第136-145页 |
| Appendix I. Genes of P. syringae MB03 constituting core genome | 第145-165页 |
| List of figures | 第165-167页 |
| List of tables | 第167-168页 |
| List of publications | 第168-169页 |
| Acknowledgements | 第169-170页 |
