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燕麦种质资源多样性及重要农艺性状的QTL分析

博士学位论文评阅人、答辩委员会签名表第3-6页
摘要第6-8页
Abstract第8-9页
Abbreviations英文缩略表第16-17页
Chapter Ⅰ Introduction第17-30页
    1.1 Importance of oat第17页
    1.2 Distribution and production of oat第17-19页
        1.2.1 Distribution and production in the world第17-19页
        1.2.2 Distribution and production in China第19页
    1.3 Origin and classification of oat第19-21页
        1.3.1 Taxonomy第19-20页
        1.3.2 Origin and evolution第20-21页
        1.3.3 Primary and secondary centers of diversity第21页
    1.4. Germplasm resources of oat第21-22页
        1.4.1 Germplasm collections第21-22页
        1.4.2 Phenotypic variation of oat germplasm resources第22页
    1.5 Molecular markers第22-26页
        1.5.1 RFLPs第23页
        1.5.2 RAPDs第23-24页
        1.5.3 AFLPs第24页
        1.5.4 SSRs第24-25页
        1.5.5 DAr Ts第25页
        1.5.6 SNPs第25-26页
    1.6 Genotypic diversity of oat assessed by molecular markers第26页
    1.7 Construction of genetic linkage maps第26-27页
    1.8 Association analysis of morphological traits and molecular markers第27-28页
    1.9 The importance and objectives of the research第28-30页
        1.9.1 Importance of the research第28页
        1.9.2 Objectives of the research第28-29页
        1.9.3 Technical road map第29-30页
Chapter Ⅱ Characterization of Morphological Traits of Oat Accessions第30-39页
    2.1 Materials and methods第30-31页
        2.1.1 plant materials第30页
        2.1.2 Field experiments第30页
        2.1.3 Data Analysis第30-31页
    2.2 Results第31-37页
        2.2.1 Basic statistics of morphological traits第31-34页
        2.2.2 Principle Component Analysis第34-35页
        2.2.3 Correlations between morphological traits of oat accessions第35页
        2.2.4 Classification and characteristics of oat accessions第35-37页
    2.3 Discussions第37-39页
        2.3.1 Morphological variation第37-38页
        2.3.2 Correlations and Principle Component Analysis of morphological traits第38页
        2.3.3 Classification and characteristics of oat accessions第38-39页
Chapter Ⅲ Assessment of Genetic Diversity in Oat Accessions by SSR Markers第39-47页
    3.1 Materials and methods第39-40页
        3.1.1 Plant materials第39页
        3.1.2 DNA Isolation and PCR amplification第39页
        3.1.3 Allele scoring第39页
        3.1.4 Data analysis第39-40页
    3.2 Results第40-45页
        3.2.1 SSR marker polymorphism第40-42页
        3.2.2 Genetic variation among oat accessions第42-43页
        3.2.3 Genetic relationships among oat accessions第43-45页
        3.2.4 Genetic structure of oat accessions第45页
    3.3 Discussions第45-47页
        3.3.1 SSR Polymorphism and allelic frequencies第45-46页
        3.3.2 Genetic variation and relationships of oat accessions from different countries第46-47页
Chapter Ⅳ Phenotypic-Genotypic Association Analysis on Oat Germplasm第47-52页
    4.1 Materials and methods第47页
        4.1.1 Plant materials第47页
        4.1.2 Data records第47页
        4.1.3 Data analysis第47页
    4.2 Results第47-50页
    4.3 Discussions第50-52页
        4.3.1 Reliable relations between morphological traits and molecular markers第50页
        4.3.2 Traits with multiple markers第50页
        4.3.3 Co-located markers for traits第50-52页
Chapter Ⅴ Construction of Genetic Linkage Map and Analysis of QTLs forNaked Grain Type in Oat第52-57页
    5.1 Materials and Methods第52-53页
        5.1.1 Construction of F2 population第52页
        5.1.2 Phenotypic identification of F2 population第52页
        5.1.3 DNA extraction and analyses of SSR genotype第52页
        5.1.4 The Data Analysis第52-53页
    5.2 Results第53-55页
        5.2.1 Trait performance of parents and F2 population第53页
        5.2.2 Construction of genetic linkage map第53-55页
        5.2.3 QTL analysis第55页
    5.3 Discussions第55-57页
        5.3.1 Genetic linkage map with SSR markers第55-56页
        5.3.2 QTLs on naked grain type and associated markers第56页
        5.3.3 Oat genetic linkage groups and QTLs第56-57页
Chapter Ⅵ Overall Conclusions第57-59页
    6.1 Phenotypic variation of oat accessions第57页
    6.2 Genetic diversity of oat accessions revealed by SSR markers第57-58页
    6.3 Phenotypic-genotypic association of oat germplasm第58页
    6.4 Genetic linkage map and QTL analysis on naked grain type第58-59页
References第59-65页
Annexes第65-86页
Acknowledgements第86-87页
Author Resume第87页

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