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Repellency of Essential Oils Against the Rice Pest Niphotettix Cincticeps and Assessement of Their Potential Effect on the Natural Enemy Licossa Pseudoannulata

摘要第7-10页
Abstract第10-13页
Chapter Ⅰ. Introduction第14-23页
    1.1 Plant based bio-pesticide第14-17页
        1.1.1 Obtention of essential oils第14页
        1.1.2 The growing concern about using essential oils第14-15页
        1.1.3 Classification of plant pesticides第15-17页
    1.2 Rice production and challenge in China第17-18页
    1.3 Niphotettix cincticeps (Hemiptera: Cicadellidae)第18-19页
    1.4 Licossa pseudoannulata (Araneae:Lycosidae)第19-21页
    1.5 Detoxification enzymes第21页
    1.6 Objectives of this research第21-23页
Chapter Ⅱ.Materials and Methods第23-36页
    2.1 Insect colony第23页
    2.2 Spider第23-24页
    2.3 Essential oil extraction第24-26页
    2.4 Essential oils dissolution第26页
    2.5 Repellency of essential oils against adult and nymph leafhoppers in the laboratory第26-27页
    2.6 Repellency of essential oils against adult and nymph leafhoppers in the greenhouse第27-28页
    2.7 Repellency of some major compounds against adult and nymph leafhoppers in thelaboratory and greenhouse第28-29页
    2.8 Enzyme activities of N. cincticeps after essential oil treatment第29页
    2.9 Bioassay of essential oils on the natural enemy Licosa pseudoannulata in thelaboratory conditions第29-32页
    2.10 Open field bioassays第32-34页
    2.11 Gas chromatography-mass spectrometry (GC-MS)analysis第34-35页
    2.12 Data analysis第35-36页
Chapter Ⅲ.Results第36-64页
    3.1 Laboratory assays第36-43页
        3.1.1 Screening of twelve essential oils第36-39页
        3.1.2 Repellency of Piper nigrum, Litsea cubeba, Zanthoxylum bungeanum,Hypericum hemsleyanum, Stemona japonica and Curcuma longa in the laboratory第39-43页
    3.2 Greenhouse assays第43-47页
        3.2.1 Repellency of Piper nigrum, Litsea cubeba, Zanthoxylum bungeanum,Hypericum hemsleyanum, Stemona japonica and Curcuma longa in the greenhouse第43-47页
    3.3 GC-MS analysis第47-49页
    3.4 Repellency of major compounds against leafhoppers in the laboratory andgreenhouse第49-54页
    3.5 Enzyme activities第54-55页
    3.6 Effect of essential oils on the spider Licosa pseudoannulata in the laboratory第55-61页
        3.6.1 Chemotaxis assay第55-57页
        3.6.2 Effect of essential oils on predation第57-61页
    3.7 Open field assays第61-64页
        3.7.1 Repellency effect of P. nigrum, L. cubeba and the mixture of two againstleafhoppers in the open field第61-62页
        3.7.2 Behavioral response of L. pseudoannulata on P. nigrum, L. cubeba and themixture of two oils in field assay第62-64页
Chapter Ⅳ. Discussion第64-74页
    4.1 Effectiveness of essential oils in repelling the rice pest N. cincticeps第64-67页
    4.2 Essential oil compositions第67页
    4.3 Effect of chemical compounds第67-68页
    4.4 Effect of essential oils on detoxification enzymes第68-69页
    4.5 Effect of essential oils on spider第69-71页
    4.6 Strength and challenge by using this strategy第71-73页
    4.7 Limitation of the method第73-74页
Chapter Ⅴ. Conclusion and perspective第74-76页
Reference cited第76-89页
Pulications list第89-90页
Acknowledgement第90-92页

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