| 摘要 | 第6-8页 |
| Abstract | 第8-9页 |
| Chapter 1 Introduction | 第13-59页 |
| 1.1 Current Status of Research and Application in Vascular Stents | 第13-26页 |
| 1.1.1 Bare metal stents | 第14-15页 |
| 1.1.2 Drug eluting stents | 第15-19页 |
| 1.1.3 Biodegradable stents | 第19-22页 |
| 1.1.4 EPC-capture stents | 第22-23页 |
| 1.1.5 Time sequence functional stents | 第23-26页 |
| 1.2 Surface Modification of Cardiovascular Materials and Implants | 第26-46页 |
| 1.2.1 Blood-biomaterial interfacial events and interaction mechanism | 第27-29页 |
| 1.2.2 Surface modification for improving hemocompatibility | 第29-38页 |
| 1.2.3 Biomimic endothelialization promoted surface modification | 第38-46页 |
| 1.3 Research Progress of Plasma Polymerization in Biomedical Materials | 第46-56页 |
| 1.3.1 A brief introduction of plasma and plasma polymerization | 第46-48页 |
| 1.3.2 Alternative deposition of different functional groups | 第48-53页 |
| 1.3.3 Plasma polymerization surface modification for biomedical materials | 第53-56页 |
| 1.4 Significance and Objectives, Dissertation Layout and Technical route | 第56-59页 |
| 1.4.1 Significance and objectives of research | 第56-57页 |
| 1.4.2 Dissertation layout | 第57-58页 |
| 1.4.3 Technical route | 第58-59页 |
| Chapter 2 Multifunctional Plasma Polymerized Film:Towards Better AnticorrosionProperty, Enhanced Cellular Growth Ability, and Attenuated Inflammatory Responses | 第59-82页 |
| 2.2 Introduction | 第60-61页 |
| 2.3 Experimental Section | 第61-65页 |
| 2.3.1 Chemicals and reagents | 第61页 |
| 2.3.2 Preparation of plasma polymerized films | 第61-62页 |
| 2.3.3 Surface characterization | 第62页 |
| 2.3.4 Quantification of amine groups by acid orange Ⅱ | 第62-63页 |
| 2.3.5 Electrochemical corrosion tests | 第63页 |
| 2.3.6 Protein adsorption via micro-BCA assays and QCM-D measurement | 第63-64页 |
| 2.3.7 In vitro cellular responses | 第64页 |
| 2.3.8 Anti-inflammatory effects | 第64-65页 |
| 2.3.9 In vivo animal tests | 第65页 |
| 2.3.10 Statistical analysis | 第65页 |
| 2.4 Results | 第65-75页 |
| 2.4.1 Surface characterization | 第65-69页 |
| 2.4.2 Electrochemical corrosion measurements | 第69-70页 |
| 2.4.3 Protein adsorption | 第70-71页 |
| 2.4.4 Cell Morphology, adhesion and viability | 第71-73页 |
| 2.4.5 Macrophage response and cytokine release | 第73-74页 |
| 2.4.6 In vivo animal study | 第74-75页 |
| 2.5 Discussion | 第75-81页 |
| 2.6 Conclusion | 第81-82页 |
| Chapter 3 Improvement of Corrosion Resistance and Biocompatibility ofBiodegradable Metallic Vascular Stent via Plasma Allylamine Polymerized Coating | 第82-99页 |
| 3.2 Introduction | 第83-84页 |
| 3.3 Experimental Section | 第84-87页 |
| 3.3.1 Chemicals and reagents | 第84-85页 |
| 3.3.2 Preparation of plasma polymerized films | 第85页 |
| 3.3.3 Surface characterization of PPAam coated MgZnMn and Fe samples | 第85页 |
| 3.3.4 Electrochemical tests and analysis of corrosion products | 第85-86页 |
| 3.3.5 ECs responses in direct culture with biodegradable coated metals | 第86页 |
| 3.3.6 Morphology examination and balloon expansion tests | 第86-87页 |
| 3.4 Results and Discussion | 第87-97页 |
| 3.4.1 Physico-chemical properties of PPAam coated MgZnMn and Fe | 第87-90页 |
| 3.4.2 Electrochemical corrosion behavior and analysis of corrosion products | 第90-94页 |
| 3.4.3 Adhesion and proliferation of ECs | 第94-96页 |
| 3.4.5 Morphology examination and balloon expansion tests | 第96-97页 |
| 3.5 Conclusions | 第97-99页 |
| Chapter 4 Immobilization of DNA Aptamers via Plasma Polymerized Allylamine Filmto Construct an Endothelial Progenitor Cell-Capture Surface | 第99-121页 |
| 4.2 Introduction | 第100-101页 |
| 4.3 Experimental and Section | 第101-105页 |
| 4.3.1 Chemicals and reagents | 第101页 |
| 4.3.2 Preparation of plasma polymerized films | 第101-102页 |
| 4.3.3 Immobilization of the EPC-aptamer | 第102页 |
| 4.3.4 Surface characterization | 第102页 |
| 4.3.5 QCM-D real time monitoring of aptamer immobilization | 第102-103页 |
| 4.3.6 Platelet adhesion and morphology | 第103页 |
| 4.3.7 Cell isolation,culture and identification | 第103-104页 |
| 4.3.8 Cell capture and proliferation behavior | 第104页 |
| 4.3.9 Competitive adhesion of EPC/EC and EPC/SMC via co-culture | 第104-105页 |
| 4.3.10 Statistical analysis | 第105页 |
| 4.4 Results and Discussion | 第105-119页 |
| 4.4.1 Surface morphology roughness and wettability | 第105-106页 |
| 4.4.2 Chemical surface compositions and quantitative characteristics | 第106-110页 |
| 4.4.3 Platelet adhesion and morphology | 第110-111页 |
| 4.4.4 Selective cell attachment and proliferation | 第111-119页 |
| 4.5 Conclusion | 第119-121页 |
| Chapter 5 In-situ Release of Nitric Oxide Combined Heparinized Plasma PolymerizedAllylamine Films for Constructing Multifunctional Hemocompatible Surface andAccelerating Endothelialization | 第121-147页 |
| 5.2 Introduction | 第122-124页 |
| 5.3 Experimental Section | 第124-129页 |
| 5.3.1 Chemicals and reagents | 第124页 |
| 5.3.2 Preparation of plasma polymerized films | 第124页 |
| 5.3.3 Sequential immobilization of heparin and SeCA | 第124页 |
| 5.3.4 Quantification of amine groups | 第124-125页 |
| 5.3.5 Analysis of chemical structure and compositions | 第125页 |
| 5.3.6 QCM-D real time monitoring of sequential immobilization process | 第125页 |
| 5.3.7 Catalytic release of NO behavior | 第125-126页 |
| 5.3.8 cGMP analysis, anti-Xa assays and whole blood culture | 第126-127页 |
| 5.3.9 ECs, SMCs and EPCs culture | 第127页 |
| 5.3.10 Adhesion and proliferation of ECs, SMCs and EPCs | 第127-128页 |
| 5.3.11 Effect of NO on mobilization of EPCs via transwell plate | 第128-129页 |
| 5.3.12 Competitive adhesion of EC/SMC and EPC/SMC via co-culture | 第129页 |
| 5.3.13 Statistical analysis | 第129页 |
| 5.4 Results and Discussion | 第129-145页 |
| 5.4.1 Sequential coimmobilization of heparin and SeCA on PPAam coating | 第129-131页 |
| 5.4.2 Dynamic detection of NO release from PPAam-Hep-SeCA surface | 第131-133页 |
| 5.4.3 cGMP analysis, anti-Xa assay and whole blood culture tests | 第133-136页 |
| 5.4.4 Synergistic effects of heparin and NO on vascular cell growth behavior | 第136-145页 |
| 5.5 Conclusion | 第145-147页 |
| Chapter 6 Summary and Future Research | 第147-152页 |
| 6.1 Summary of Thesis | 第147-148页 |
| 6.2 Limitation of Current Research | 第148-150页 |
| 6.3 Recommendation for Future Research | 第150-152页 |
| Acknowlegements | 第152-154页 |
| References | 第154-178页 |
| Abbreviation List | 第178-179页 |
| Scientific achievements | 第179-182页 |
| 1. Publications | 第179-181页 |
| 2. Patents | 第181页 |
| 3. Confernce Presentations | 第181-182页 |
| 4. Foundation | 第182页 |
| 5. Awards and Honors | 第182页 |
