| 摘要 | 第8-10页 |
| ABSTRACT | 第10-11页 |
| LIST OF ABBREVIATIONS | 第12-13页 |
| CHAPTER Ⅰ.LITERATURE REVIEW. LITERATURE REVIEW | 第13-35页 |
| 1.1 Strigolactones (SLs): An overview | 第13-15页 |
| 1.2 Chemical structure of SLs | 第15-17页 |
| 1.3 SLs biosynthesis pathway | 第17-22页 |
| 1.3.1 SLs perception | 第20-21页 |
| 1.3.2 SLs transports | 第21-22页 |
| 1.4 SLs biological functions | 第22-28页 |
| 1.4.1 SLs signaling in the shoot branches | 第22-24页 |
| 1.4.2 SLs role in plants roots shaping and roots hairs architecture | 第24-26页 |
| 1.4.3 Role of SLs in nodulation | 第26-28页 |
| 1.5 SLs interaction with other plants hormones, | 第28-31页 |
| 1.5.1 SLs interaction with other plants hormones, ABA, JA and in plants development | 第28-29页 |
| 1.5.2 SLs interaction with auxin | 第29-31页 |
| 1.6 More axillary growth (MAXs) genes and their function in SLs biosynthesis and signaling pathway | 第31-33页 |
| 1.6.1 Function of MAX1 in SLs biosynthesis | 第31-32页 |
| 1.6.2 Function of MAX2 in SLs signaling | 第32页 |
| 1.6.3 Function of MAX3 and MAX4 in SLs biosynthesis | 第32-33页 |
| 1.7 Biological application of strigolactones | 第33-34页 |
| 1.8 Objectives of the study | 第34-35页 |
| CHAPTER Ⅱ.MATERIALS AND METHODS | 第35-47页 |
| 2.1 Identification of soybean Gm MAXs genes family and in silico analysis | 第35页 |
| 2.2 Plant materials and growth conditions | 第35-36页 |
| 2.3 Gene cloning and vector construction | 第36-37页 |
| 2.4 T-easy Ligation | 第37页 |
| 2.5 BP reaction | 第37-38页 |
| 2.6 LR reaction | 第38页 |
| 2.7 Gel electrophoresis and purification of PCR product | 第38-39页 |
| 2.8 Ecoli transformation | 第39页 |
| 2.9 Plasmid Isolation | 第39页 |
| 2.10 Transformation | 第39-40页 |
| 2.10.1 Arabidopsis transformation and mutant complementation | 第39-40页 |
| 2.11 Evaluation of branches number, leaf size, and leaf shape | 第40页 |
| 2.12 Analysis of hypocotyl length, primary root and root hair length | 第40-41页 |
| 2.13 SLs biosynthesis and SLs signaling atmaxs mutant drought stress tolerance response assay | 第41页 |
| 2.14 Soybean hairy root induction | 第41-42页 |
| 2.14.1 In vivo hairy root induction | 第41-42页 |
| 2.15 In -vitro hairy root induction | 第42-43页 |
| 2.15.1 Gm MAXs transgenic hairy root nodulation assay | 第42页 |
| 2.15.2 Rhizobia infection and Effects of expression in early stage nodulation genes | 第42-43页 |
| 2.16 RNA isolation, c DNA library construction for Illumina deep sequencing | 第43页 |
| 2.17 Analysis of gene expression | 第43-44页 |
| 2.18 Hormone quantification analysis | 第44页 |
| 2.19 Subcellular localization of Gm MAX1a, Gm MAX2a, Gm MAX3b and Gm MAX4a proteins | 第44-45页 |
| 2.20 Bioinformatics analysis | 第45页 |
| 2.21 Statistical analysis | 第45-46页 |
| 2.22 Protein Accession number | 第46-47页 |
| CHAPTER Ⅲ.RESULTS AND ANALYSIS | 第47-87页 |
| 3.1 Identification of At MAX homologues from soybean genome | 第47-52页 |
| 3.2 Tissue-specific expression patterns of Gm MAX genes | 第52-54页 |
| 3.3 Genetic complementation of Gm MAXs genes in Arabidopsis | 第54-62页 |
| 3.3.1 Functions of Gm MAX1a when expressed in Arabidopsis | 第54-56页 |
| 3.3.2 Functional analysis of Gm MAX2a in Arabidopsis | 第56-58页 |
| 3.3.3 Functional analysis of Gm MAX3b in Arabidopsis | 第58-60页 |
| 3.3.4 Genetic complementation of Arabidopsis atmax4-1 by Gm MAX4a | 第60-62页 |
| 3.4 Gm MAXs complemented the atmax mutants’ root lengthdefect phenotypes | 第62-64页 |
| 3.5 Gm MAXs complemented the atmax mutants’ defective root hair phenotypes | 第64-65页 |
| 3.6 Gm MAXs complemented the atmax mutants’ stem trichome defect phenotypes. .. | 第65-66页 |
| 3.7 SLs biosynthesis and signaling genes in Arabidopsis max mutants and soybean root under (drought) stress response | 第66-68页 |
| 3.8 Gm MAXs expression in Arabidopsis altered hormones levels | 第68-69页 |
| 3.9 Overexpression of Gm MAXs SLs biosynthesis and signaling genes in soybean transgenic hairy roots and analysis of phenotype observation of roots hairs | 第69-71页 |
| 3.10 Transcript response of strigolactone biosynthesis and signaling gene Gm MAX1a, Gm MAX2a, Gm MAX3b and Gm MAX4a upon B. Japanicum infection in wild-type seedlings | 第71-72页 |
| 3.11 SLs biosynthesis and signaling genes altered at different stages of nodulation | 第72-74页 |
| 3.12 Overexpression Gm MAX1a, Gm MAX2a, Gm MAX3b, Gm MAX4a and knockdown of Gm MAX3b-KD in soybean hairy roots altered nodulation | 第74-77页 |
| 3.13 Effects of SLs biosynthesis and SLs signaling Gm MAXs genes overexpression on plant hormones | 第77-80页 |
| 3.14 Global gene expression changed in Gm MAX3b-OE and Gm MAX3b-KD soybean hairy roots | 第80-82页 |
| 3.15 Effects of Gm MAX3b-OE and Gm MAX3b-KD on auxin biosynthesis genes | 第82-84页 |
| 3.16 Gm MAX3b-OE and Gm MAX3b-KD hairy roots altered the endogenous hormone levels | 第84-85页 |
| 3.17 Subcellular localization of Gm MAXs protein | 第85-87页 |
| 3.17.1 Subcellular localization of Gm MAX1a, Gm MAX2a, Gm MAX3b and Gm MAX4a genes | 第86-87页 |
| CHAPTER Ⅳ.DISCUSSION | 第87-93页 |
| 4.1 Gm MAXs have conserved functions similar to their Arabidopsis counterparts | 第87-88页 |
| 4.2 SLs has an important role in enhancing drought tolerance to atmaxs | 第88页 |
| 4.3 Gm MAX3b regulated hormone biosynthesis when expressed in Arabidopsis | 第88-89页 |
| 4.4 Gm MAX1a, Gm MAX2a, Gm MAX3b and Gm MAX4a regulated soybean hairy root nodulation | 第89-90页 |
| 4.5 Gm MAX3b overexpression and knockdown changed the expression of subsets of the gene in soybean hairy roots | 第90-91页 |
| 4.6 Gm MAX3b may modulate SLs, auxin and other hormone signaling in soybean hairy roots to affect soybean nodulation | 第91-93页 |
| CHAPTER Ⅴ.CONCLUSION | 第93-94页 |
| Future work on Strigolactones | 第94-95页 |
| REFERENCES | 第95-107页 |
| Appendices | 第107-123页 |
| Appendix 1 | 第107-111页 |
| Appendix 2 | 第111页 |
| Appendix 3 | 第111-114页 |
| Appendix4 | 第114-123页 |
| ACKNOWLEDGEMENT | 第123-124页 |
| List of publications | 第124-125页 |
