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D基因组二倍体棉种D3-d和D3-k种间杂交种的SSR连锁图谱

摘要第6-7页
ABSTRACT第7页
ABBREVIATION第13-14页
DEDICATION第14-15页
CHAPTER ONE INTRODUCTION第15-33页
    1.1 The Cotton第15-21页
        1.1.1 Cotton morphology第15-16页
        1.1.2 Origin of cotton第16-17页
        1.1.3 Countries with Highest Production of Cotton第17-19页
        1.1.4 Agronomic properties for cotton production第19页
        1.1.5 Growth and development of a cotton plant第19-20页
        1.1.6 Uses of Cotton第20-21页
    1.2 Wild cotton species第21-22页
        1.2.1 Importance of wild cotton species in breeding第21-22页
        1.2.2 The use of wild cotton species: G. davidsonii and G.klotzschianum第22页
    1.3 Breeding and breeding strategies in cotton production第22-25页
        1.3.1 Development of parental lines第22-24页
        1.3.2 Genetic markers第24-25页
    1.4 Genetic Mapping第25-29页
        1.4.1 Significance of genetic mapping第25-26页
        1.4.2 Importance of SSR markers in screening and genotyping of population第26-27页
        1.4.3 Identification of polymorphism using SSR markers第27-28页
        1.4.4 Advantages of the use of EST-SSR markers第28-29页
    1.5 Distribution of genes on the D genome第29-31页
    1.6 Significance of gene discovery and mining in cotton genomes第31-32页
    1.7 Main objective of the research第32-33页
        1.7.1 Specific objectives第32-33页
CHAPTER TWO MATERIALS AND METHODS第33-37页
    2.1 Plant materials第33页
    2.2 DNA extraction, quantification and qualification第33-34页
    2.3 Electrophoresis第34页
    2.4 Screening SSR markers for polymorphism第34页
    2.5 Genotyping using the SWU polymorphic primers第34-35页
    2.6 Software's for construction of genetic map第35页
    2.7 Collinearity Analysis第35-36页
    2.8 Gene Mining,GO Functional Annotation,and Expression Analysis第36-37页
CHAPTER THREE RESULTS第37-57页
    3.1 Marker information第37页
    3.2 General information about the genetic map第37-43页
    3.3 Segregation distortion第43-46页
    3.4 Collinearity analysis第46-48页
    3.5 Collinearity Analysis of the Genetic Map and the Physical Map of the (Dt) Sub-Genome第48-49页
    3.6 Gene Mining,GO Functional Annotation, and RNA sequence expressionanalysis第49-57页
        3.6.1 Gene mining第49页
        3.6.2 Physiochemical properties and gene structure analysis of the mined genes withinthe SSR regions第49-50页
        3.6.4 Gene Ontology(GO)analysis第50-51页
        3.6.5 RNA expression analysis第51-57页
CHAPTER FOUR DISCUSSION第57-66页
    4.1 Parental materials第57页
    4.2 Polymorphism using EST-SSR markers第57-59页
    4.3 The genetic map constructed from wild species of the D genome第59-60页
    4.4 Segregation distortion in diploid cotton of D genome第60-61页
    4.5 Collinearity in D Genome cotton第61-63页
    4.6 Gene Mining,GO Annotation and Expression第63-66页
        4.6.1 Gene Mining第63页
        4.6.2 Physiological properties of mined genes第63-65页
        4.6.3 GO Annotations第65-66页
CONCLUSION第66-67页
REFERENCES第67-81页
ACKNOWLEDGEMENT第81-82页
CURRICULUM VITAE第82-84页

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