| ACKNOWLEDGMENTS | 第1-8页 |
| ABBREVIATIONS | 第8-10页 |
| 摘要 | 第10-12页 |
| Abstract | 第12-15页 |
| 1 INTRODUCTION | 第15-25页 |
| ·Two Hit Hypothesis | 第15页 |
| ·Molecular Mechanism of Oxidative Stress in Inflammation | 第15-16页 |
| ·Molecular Mechanism of Classical LPS signaling | 第16-18页 |
| ·Progressions Made at Understanding Mechanism of LPS Signaling Reprogrammed by Antecedent Oxidative Stress | 第18-20页 |
| ·Systematically Study the Classical and the Reprogrammed LPS Pathway | 第20-21页 |
| ·Drawbacks of Previous Studies on LPS Signal Transduction | 第20页 |
| ·Advantages of Application of Proteomic Study to Signal Transduction | 第20-21页 |
| ·Current Opinions of Phosphoproteomic Techniques | 第21-25页 |
| 2 METHODOLOGIES OF PHOSPHOPROTEOMICS | 第25-37页 |
| ·Summary | 第25页 |
| ·Cell Culture | 第25-26页 |
| ·Testing Cell's Responsibility to LPS by Liquid Chip | 第26-27页 |
| ·Phosphoprotein Enrichment Procedure | 第27-29页 |
| A. Extracting Proteins from Cells | 第27-28页 |
| B. Column Enrichment | 第28-29页 |
| ·Desaiting of Phosphoproteins | 第29-30页 |
| ·2-D Electrophoresis | 第30-31页 |
| A. Isoelectric Focusing | 第30-31页 |
| B. Equilibration and the Second Dimension | 第31页 |
| ·Combined Staining Approach Using Both "Blue Silver" Coomassie Blue Staining and "Modified Blum" Silver Staining | 第31-32页 |
| ·Analysis of 2-D Gels | 第32-34页 |
| ·Preparation of Samples for Mass Spectrometry | 第34-35页 |
| A. Destaining of silver stained gel pieces | 第34页 |
| B. In Gel Digestion | 第34-35页 |
| C. Peptide extraction | 第35页 |
| ·Mass Spectrometry Analysis and Database Searching of The Determined PMF | 第35-37页 |
| 3 RESULTS AND DISCUSSIONS | 第37-82页 |
| ·Results | 第37-71页 |
| ·Phosphoproteome Profile of Thp-1 Cells After LPS Stimulation | 第38-39页 |
| ·Oxidative Stress Induced Changes in Phosphoproteome of LPS Stimulated Thp-1 Cells | 第39-44页 |
| ·Identification of Candidate Targets of LPS Signalling Pathway Reprogrammed by Antecedent Oxidative Stress | 第44-66页 |
| ·Possible Functional Roles of the Identified Phosphoproteins | 第66-71页 |
| ·Discussion | 第71-79页 |
| ·Advantages and Limitations of This Study | 第71-73页 |
| ·Possible Functional Roles Proteasome and NMP-238 Plays in Reprogramming LPS Signalling Pathway | 第73-79页 |
| ·Conclusion Remarks | 第79-82页 |
| 4 IMPLICATIONS AND FUTURE DIRECTIONS | 第82-85页 |
| REFERENCES | 第85-89页 |
