| 摘要 | 第9-11页 |
| ABSTRACT | 第11-13页 |
| LIST OF ABBREVIATIONS | 第14-16页 |
| PART I. LITERATURE REVIEW | 第16-34页 |
| CHAPTER 1. HERBAL MEDICINE USAGE IN THERAPEUTICS | 第16-20页 |
| 1 Overview of herbal medicine usage in therapeutics | 第16-18页 |
| 1.1 Herbal medicine usage in China and Asia | 第16-17页 |
| 1.2 Herbal medicine usage in Africa | 第17页 |
| 1.3 Herbal medicine usage in the rest of the world | 第17-18页 |
| 2 The future of herbal medicine in health care delivery | 第18-20页 |
| CHAPTER 2. HERBAL EPIMEDIUM AND REPRODUCTIVE DEVELOPMENT | 第20-34页 |
| 1 Herbal Epimedium | 第20-29页 |
| 1.1 Sources and distribution | 第20页 |
| 1.2 Botanical description and species | 第20-22页 |
| 1.3 Ethnopharmacological, traditional and current uses | 第22-23页 |
| 1.4 Chemical composition of herbal Epimedium | 第23-25页 |
| 1.5 Processing and quality control methods | 第25-26页 |
| 1.6 Estrogen-like activity of herbal Epimedium | 第26-27页 |
| 1.7 Other pharmacological action of herbal Epimedium | 第27-29页 |
| 1.8 Side effects and acute toxicity of herbal Epimedium | 第29页 |
| 2 Basic biology of the mouse ovary and the regulation of reproduction | 第29-31页 |
| 3 Ovarian follicle development and oocyte maturation | 第31-32页 |
| 4 Objectives | 第32-34页 |
| PART Ⅱ. EXPERIMENTAL STUDY | 第34-66页 |
| CHAPTER 3. EFFECTS OF EPIMEDIUM ON MOUSE OVARY AND UTERUSDEVELOPMENT | 第34-46页 |
| 1 Materials and methods | 第35-39页 |
| 1.1 Animals | 第35页 |
| 1.2 Reagents | 第35-36页 |
| 1.3 EP medicine, fixatives and tissue culture media preparations | 第36-38页 |
| 1.4 The main instruments and equipment | 第38-39页 |
| 2 Experimental design and work | 第39-41页 |
| 2.1 Ovarian tissue preparation | 第39-40页 |
| 2.2 Oocyte culture in vitro | 第40页 |
| 2.3 Determination of reproductive index (RI) | 第40-41页 |
| 2.4 Preparation of histological sections of mice ovaries | 第41页 |
| 2.5 Statistical analysis | 第41页 |
| 3 Results | 第41-43页 |
| 3.1 Effects of EP on body weight of mice | 第41页 |
| 3.2 Effects of EP on reproductive index | 第41-42页 |
| 3.3 Oocyte culture evaluation | 第42-43页 |
| 3.4 Histological evaluation | 第43页 |
| 4 Discussion | 第43-46页 |
| CHAPTER 4. EFFECTS OF EPS ON MOUSE OVARY AND UTERUS DEVELOPMENT | 第46-56页 |
| 1 Materials and methods | 第47-48页 |
| 1.1 Animals | 第47页 |
| 1.2 Reagents | 第47页 |
| 1.3 EPS medicine, fixatives and tissue culture media preparations | 第47-48页 |
| 1.4 The main instruments and equipment | 第48页 |
| 2 Experimental design | 第48-50页 |
| 2.1 Ovarian tissue preparation | 第49页 |
| 2.2 Oocyte culture in vitro | 第49-50页 |
| 2.3 Determination of reproductive index (RI) | 第50页 |
| 2.4 Preparation of histological sections of mice ovaries | 第50页 |
| 2.5 Statistical analysis | 第50页 |
| 3 Results | 第50-53页 |
| 3.1 Effects of EPS on body weight of mice | 第51页 |
| 3.2 Effects of EPS on reproductive index | 第51页 |
| 3.3 Oocyte culture evaluation | 第51-52页 |
| 3.4 Histological evaluation | 第52-53页 |
| 4 Discussion | 第53-56页 |
| CHAPTER 5. EFFECTS OF ICA ON MOUSE OVARY AND UTERUS DEVELOPMENT | 第56-66页 |
| 1 Materials and methods | 第57-59页 |
| 1.1 Animals | 第57页 |
| 1.2 Reagents | 第57页 |
| 1.3 ICA medicine, fixatives and tissue culture media preparations | 第57-58页 |
| 1.4 The main instruments and equipment | 第58-59页 |
| 2 Experimental design | 第59-61页 |
| 2.1 Ovarian tissue preparation | 第59页 |
| 2.2 Oocyte culture in vitro | 第59-60页 |
| 2.3 Determination of reproductive index (RI) | 第60页 |
| 2.4 Preparation of histological sections of mice ovaries | 第60页 |
| 2.5 Statistical analysis | 第60-61页 |
| 3 Results | 第61-63页 |
| 3.1 Effects of ICA on body weight of mice | 第61页 |
| 3.2 Effects of ICA on reproductive index | 第61-62页 |
| 3.3 Oocyte culture evaluation | 第62页 |
| 3.4 Histological evaluation | 第62-63页 |
| 4 Discussion | 第63-66页 |
| SUMMARY AND CONCLUSION | 第66-68页 |
| REFERENCES | 第68-80页 |
| ACKNOWLEDGEMENT | 第80-82页 |
| DEDICATION | 第82页 |
