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柿果实品质特性、香气成分分析及醇酰基转移酶基因(DkAAT1)的表达研究

LIST OF ABBREVIATIONS第11-13页
Abstract第13-16页
摘要第17-20页
1. Introduction第20-23页
2. Literature Review第23-46页
    2.1. History and culture of persimmon trees in China第23页
    2.2. Origin and distribution of persimmon第23-24页
    2.3. Persimmon economic importance第24-28页
    2.4. Persimmon nutrition and medicinal value第28页
    2.5. Clasification of persimmon cultivars第28-29页
    2.6. Aroma volatile composition and their biological characteristic of major fruits第29页
    2.7. Classification of volatile compounds in fruit flavor第29-31页
    2.8. Volatile compounds and their biological characteristic of persimmon fruits第31页
    2.9. Factors influencing volatile composition第31-37页
        2.9.1. Genetics第31-32页
        2.9.2. Maturity第32-34页
        2.9.3. Pre-harvest factors第34页
        2.9.4. Post-harvest handling第34-37页
    2.10. Volatile aroma compounds biosynthestic pathways and related enzymes第37-46页
        2.10.1. Fatty acids pathway第37-40页
        2.10.2. Amino acid pathway第40-42页
        2.10.3. Terpenoids pathway第42-44页
        2.10.4. Carotenoid Pathway第44-46页
3. Analysis of Main Quality Characteristics and Aroma Volatile Composition of Persimmon(Diospyros kaki L.) Fruits第46-113页
    3.1. Introduction第46-48页
    3.2. Materials and methods第48-53页
        3.2.1. Plant materials and sampling第48-50页
        3.2.2. Fruit quality assessment第50-51页
        3.2.3. Sampling and analysis of volatile compounds by headspace- SPME第51-52页
        3.2.4. Assay of AAT Activity第52页
        3.2.5. Statistical Analysis第52-53页
    3.3. Results第53-107页
        3.3.1. Fruit quality assessment第53-61页
        3.3.2. Production of aroma volatiles of persimmon fruits第61-97页
            3.3.2.1. Production of aroma volatiles of different cultivars of persimmon第61-95页
            3.3.2.2. Production ofvolatiles during different ripening stages of persimmon fruits第95-97页
        3.3.3. AAT activity during ripening of persimmon fruit第97-107页
    3.4. Discussion第107-113页
4. Identification and Expression of Alcohol Acyltransferase (DKAAT1) Responsible for EsterBiosynthesis in Persimmon (Diospyros kaki L.) Fruits第113-142页
    4.1. Introduction第113-114页
    4.2. Material and Methods第114-128页
        4.2.1. Plant Materials第114-115页
        4.2.2. Isolation and characterization of DkAAT1第115-123页
        4.2.3. Genomic DNA Isolation and DNA Gel-Blot Analysis第123-125页
        4.2.4. Gene expression analysis第125-128页
    4.3. Results第128-139页
        4.3.1. Cloning and sequence analysis of DkAAT1 cDNA第128-131页
        4.3.2. Protein Prediction and Phylogenetic Analysis第131页
        4.3.3. DNA gel-blot analysis第131-138页
        4.3.4. Transcriptional analysis of DkAAT1第138-139页
    4.4. Discussion第139-142页
5. Transcriptome Analysis of Persimmon (Diospyros kaki L.) Fruits第142-156页
    5.1. Introduction第142-143页
    5.2. Material and Methods第143-146页
        5.2.1. Sample collection and preparation第143页
        5.2.2. RNA extraction, quantification and qualification第143页
        5.2.3. Library preparation for Transcriptome sequencing第143-144页
        5.2.4. Clustering and sequencing第144页
        5.2.5. Data analysis第144-146页
    5.3. Results第146-153页
        5.3.1. RNA-Seq and de novo assembly第146-147页
        5.3.2. Characteristics of the homology search of persimmon unigenes第147-153页
    5.4. Discussion第153-156页
6. Conclusion第156-157页
References第157-185页
DEDICATION第185-186页
ACKNOWLEDGEMENTS第186-187页
PUBLICATIONS第187-188页

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