| Abstract (In Chinese) | 第4-7页 |
| Abstract (In English) | 第7-8页 |
| Chapter 1 Introduction | 第12-20页 |
| 1.1 Background and significance of this study | 第12-13页 |
| 1.2 Biosensors that respond to β-estradiol | 第13页 |
| 1.3 Synthetic promoter design with lex Op2 operators | 第13-15页 |
| 1.4 The basic parts used in this project | 第15-16页 |
| 1.5 Research status in this direction | 第16页 |
| 1.6 Main research content and goal | 第16-20页 |
| 1.6.1 Testing the biosensor performance with various distance between lex Op2 operators | 第17-18页 |
| 1.6.2 Testing the biosensor performance with various distance between lex Op2 operator and TATA box | 第18-20页 |
| Chapter 2 Empirical methods | 第20-36页 |
| 2.1 Laboratory machine | 第20页 |
| 2.2 Experimental materials | 第20-22页 |
| 2.2.1 Microbiology Strain | 第20-21页 |
| 2.2.2 DNA parts | 第21-22页 |
| 2.2.3 Enzymes | 第22页 |
| 2.3 Protocols | 第22-36页 |
| 2.3.1 Yeast genome extraction methods | 第22-23页 |
| 2.3.2 Bacteria transformation | 第23-24页 |
| 2.3.3 Restriction digest | 第24页 |
| 2.3.4 Gel electrophoresis | 第24-25页 |
| 2.3.5 DNA elution | 第25-26页 |
| 2.3.6 Primer preparation | 第26页 |
| 2.3.7 Blackburn PCR | 第26-27页 |
| 2.3.8 Isothermal assembly—Gibson protocol | 第27页 |
| 2.3.9 Touchdown PCR | 第27-28页 |
| 2.3.10 Making Competent Cells | 第28-29页 |
| 2.3.11 Mini preparation | 第29-30页 |
| 2.3.12 Yeast transformation | 第30-31页 |
| 2.3.13 Cryostocks | 第31-32页 |
| 2.3.14 Colony picking | 第32页 |
| 2.3.15 Yeast cell induction | 第32-33页 |
| 2.3.16 FACS measurements—BD FACSCalibur | 第33-35页 |
| 2.3.17 β -estradiol concentration curve preparation | 第35页 |
| 2.3.18 Data analysis program | 第35-36页 |
| Chapter 3 Optimization based on various promoters and activation domain | 第36-41页 |
| 3.1 Performance based on various promoter in the reporter section | 第36页 |
| 3.2 Performance based on various promoters in the sensor section | 第36-39页 |
| 3.3 Performance based on various activation domains in the sensor section | 第39-40页 |
| 3.4 Chapter brief summary | 第40-41页 |
| Chapter 4 Modification on the lex Op2 operator in the reporter section | 第41-48页 |
| 4.1 Synthetic activated promoters in the literature | 第41-42页 |
| 4.2 Performance based on various design of lex Op2 operators in the reporter section | 第42-44页 |
| 4.3 Performance based on an increasing number of lex Op2 operators | 第44-46页 |
| 4.4 Chapter brief summary | 第46-48页 |
| Chapter 5 Testing the biosensor performance with various positions of the lex Op2 operators | 第48-56页 |
| 5.1 Biosensors based on different distance between two adjacent lex Op2 operators | 第48-52页 |
| 5.1.1 Construction of biosensors with various distance between lex Op2 operators | 第48-49页 |
| 5.1.2 Performance of the biosensor with various distance between lex Aop2 operators | 第49-50页 |
| 5.1.3 Promoter comparison | 第50-52页 |
| 5.2 Biosensors based on different distance between a lex Op2 operator and the TATA box | 第52-55页 |
| 5.2.1 Construction of biosensors with various distance between a lex Op2 operator and the TATA box | 第52页 |
| 5.2.2 Performance of the biosensors with various distance between a single lex Op2 operator and the TATA box | 第52-53页 |
| 5.2.3 Promoter comparison | 第53-55页 |
| 5.3 Chapter brief summary | 第55-56页 |
| Conclusion | 第56-57页 |
| References | 第57-62页 |
| Acknowledgements | 第62页 |
