| 中文摘要 | 第4-6页 |
| ABSTRACT | 第6-7页 |
| LIST OF TABLES | 第8-9页 |
| LIST OF FIGURES | 第9-12页 |
| ABBREVIATIONS | 第12-13页 |
| CHAPTER 1: LITERATURE REVIEW | 第13-25页 |
| 1.1 Lung cancer and the role of aquaporins in cancer development | 第13-17页 |
| 1.2 The apoptotic process in cancer | 第17-21页 |
| 1.3 The process of differentiation and apoptosis in proliferating cancer cells | 第21-22页 |
| 1.4 Aquaporin 5 expression and apoptosis in cancer | 第22-25页 |
| CHAPTER 2: MATERIALS AND METHODS | 第25-33页 |
| 2.1 Tissue specimens study | 第25-28页 |
| 2.1.1 Patients and tumor material | 第25页 |
| 2.1.2 Tissue immunohistochemistry and cell diameter measurement | 第25-26页 |
| 2.1.3 The TUNEL staining method | 第26-28页 |
| 2.2 Cell culture study and materials | 第28-32页 |
| 2.2.1 ATRA induction of differentiation and the MTT proliferation study | 第29页 |
| 2.2.2 Measurement of A549 reversal of phenotype and differentiation | 第29-30页 |
| 2.2.3 A549 Cell Immunocytochemistry and Immunoflorescence | 第30-31页 |
| 2.2.4 Annexin V/PI staining and flow cytometry | 第31-32页 |
| 2.3 Statistical analysis | 第32-33页 |
| CHAPTER 3: RESULTS | 第33-46页 |
| 3.1 Tissue study | 第33-37页 |
| 3.1.1 Tissue AQP5 immunohistochemistry staining lung squamous cell carcinoma (SCC) and adenocarcinoma(AC) | 第33-34页 |
| 3.1.2 Diameter of cancer cells and mean optical density in lung squamous cell carcinoma (SCC) and adenocarcinoma (AC) | 第34-35页 |
| 3.1.3 Tissue TUNEL staining for apoptosis in lung squamous cell carcinoma (SCC) and adenocarcinoma (AC) | 第35页 |
| 3.1.4 Apoptotic indices (per 1000 cells) for lung squamous cell carcinoma (SCC) andadenocarcinoma (AC). | 第35-36页 |
| 3.1.5 Correlations between diameters, mean optical density and apoptotic indices | 第36-37页 |
| 3.2 Cell culture study | 第37-46页 |
| 3.2.1 ATRA induction of differentiation and MTT cell proliferation study | 第37-38页 |
| 3.2.2 : A549 cytomorphological changes after ATRA treatment for hrs | 第38-40页 |
| 3.2.3 : Measurement of A549 reversal of phenotype and differentiation | 第40-41页 |
| 3.2.4 : A549 cell immunohistochemistry and Immunoflourescence | 第41-44页 |
| 3.2.5 Annexin V/PI staining and flow cytometry | 第44-46页 |
| CHAPTER 4: DISCUSSION | 第46-54页 |
| CHAPTER 5: CONCLUSION | 第54-56页 |
| REFERENCES | 第56-68页 |
| ACKNOWLEDGEMENTS | 第68页 |
