| 摘要 | 第1-7页 |
| Abstract | 第7-12页 |
| CHAPTER 1.INTRODUCTION | 第12-26页 |
| ·THE HONEYBEES | 第12页 |
| ·ORIGIN AND GLOBAL DISTRIBUTION OF HONEYBEES | 第12-13页 |
| ·APIS MELIFERA AND APIS CERANA | 第13-15页 |
| ·BEEKEEPING AND ITS ECONOMIC IMPORTANCE | 第15-16页 |
| ·THE HONEYBEE BIOLOGY AND SOCIAL ORGANIZATION | 第16-20页 |
| ·General morphology of the honeybee population | 第16-17页 |
| ·Development of the Honeybee Brood | 第17-18页 |
| ·Honeybee Colony Organization and Performance | 第18-20页 |
| ·HEMOLYMPH | 第20页 |
| ·HONEYBEES DISEASES AND THEIR IMMUNITY SYSTEM | 第20-22页 |
| ·CHALLENGES IN HONEYBEE RESEARCH | 第22-23页 |
| ·HONEYBEE PROTEOMICS | 第23-24页 |
| ·RESEARCH AIMS AND HYPOTHESIS | 第24-26页 |
| CHAPTER 2.HEMOLYMPH PROTEOME ANALYSIS DURING LARVAL TOPUPAL DEVELOPMENT STAGE OF HONEYBEE WORKER (APIS MELLIFERALIGUSTICA) | 第26-54页 |
| ·SUMMARY | 第26页 |
| ·INTRODUCTION | 第26-27页 |
| ·OBJECTIVE OF THE STUDY | 第27页 |
| ·MATERIAL AND METHODS | 第27-31页 |
| ·Chemical Reagents | 第27-28页 |
| ·Biological Sample Collection | 第28页 |
| ·Protein Sample Preparation | 第28-29页 |
| ·Two-Dimensioional Gel Electrophoresis(2-DE) | 第29页 |
| ·Image Analysis | 第29页 |
| ·Tryptic Digestion for MS | 第29-30页 |
| ·Mass Spectrometry and Protein Identification | 第30-31页 |
| ·Protein Classification | 第31页 |
| ·Biological Interaction Network and Functional Enrichment Analysis | 第31页 |
| ·Test of Protein Expression by Western blot | 第31页 |
| ·RESULTS | 第31-47页 |
| ·Identification of Differentially Expressed Proteins | 第31-39页 |
| ·Qualitative Comparison of Differentially Expressed Proteins | 第39-40页 |
| ·Quantitative Comparisons of Differentially Expressed Proteins | 第40-44页 |
| ·Biological Interaction Network Analysis | 第44-45页 |
| ·Gene Ontology(GO)Functional Enrichment Analysis | 第45-46页 |
| ·Western blot Analysis | 第46-47页 |
| ·DISCUSSIONS | 第47-53页 |
| ·CONCLUSION | 第53-54页 |
| CHAPTER 3.COMPARATIVE ANALYSIS OF HEMOLYMPH PROTEOMEDURING LARVA-PUPA DEVELOPMENT STAGES BETWEEN WESTERN(APISMELIFERA LIGUSTICA)AND EASTERN(APIS CERANA CERANA)WORKERHONEYBEES | 第54-86页 |
| ·SUMMARY | 第54-55页 |
| ·INTRODUCTION | 第55-56页 |
| ·OBJECTIVES | 第56页 |
| ·MATERIAL AND METHODS | 第56-58页 |
| ·Chemical reagents | 第56页 |
| ·Biological Samples | 第56页 |
| ·Protein extraction and Two-Dimensional Gel electrophoresis | 第56-57页 |
| ·Image Acquisition and Statistical Analysis | 第57页 |
| ·Trypsin Digestion for MS and Proteins Identification by MS | 第57页 |
| ·Bioinformatics | 第57-58页 |
| ·RESULTS | 第58-79页 |
| ·Identification of differentially expressed proteins | 第58-70页 |
| ·Qualitative Comparisons of Differentially Expressed Proteins | 第70-74页 |
| ·Quantitative Comparisons of Differentially Expressed Proteins | 第74-76页 |
| ·Protein-Protein Interaction Analysis | 第76-77页 |
| ·Gene Ontology(GO)Functional Enrichment Analysis | 第77-79页 |
| ·DISCUSSIONS | 第79-85页 |
| ·CONCLUSION | 第85-86页 |
| CHAPTER 4.GENERAL CONCLUSION | 第86-87页 |
| REFERENCES | 第87-99页 |
| Acknowledgments | 第99-100页 |
| Author's Biography | 第100-103页 |
