| 摘要 | 第1-8页 |
| Abstract | 第8-14页 |
| CHAPTER Ⅰ Introduction | 第14-26页 |
| ·Light perception and photoreceptors in plant life | 第14-19页 |
| ·Phytochromes in structure and function | 第16-17页 |
| ·PIFs as key regulators of light signaling | 第17-19页 |
| ·DELLA proteins as central repressors of GA signalling | 第19-24页 |
| ·Molecular mechanism of DELLA regulated growth | 第20-21页 |
| ·DELLA proteins in plant defense | 第21页 |
| ·DELLA protein stabilization in plants | 第21-23页 |
| ·DELLAs manage ROS level in plant life under stress | 第23-24页 |
| ·Salinity and its impact on plant life | 第24页 |
| ·Expression of antioxidant genes under salt stress | 第24-25页 |
| ·Research objectives | 第25-26页 |
| CHAPTER Ⅱ Materials and Methods | 第26-34页 |
| ·Materials | 第26-28页 |
| ·Chemicals | 第26页 |
| ·Enzymes and molecular biology kits | 第26页 |
| ·Instrumentation | 第26-27页 |
| ·Antibodies | 第27页 |
| ·General buffers and solutions | 第27页 |
| ·Plant media | 第27页 |
| ·Plant material | 第27-28页 |
| ·Methods | 第28-34页 |
| ·Seed sterilization for Arabidopsis in-vitro culture | 第28页 |
| ·Growth conditions | 第28页 |
| ·Phenotypic analyses | 第28-29页 |
| ·Light treatments of plants | 第28页 |
| ·Seed germination | 第28-29页 |
| ·Root growth elongation assay | 第29页 |
| ·Salt stress survival assay | 第29页 |
| ·Hypocotyl growth inhibition assay | 第29页 |
| ·Protein extraction | 第29-30页 |
| ·Protein quantification | 第29-30页 |
| ·Preparation of Tris-Glycine SDS-Polyacrylamide Gel Electrophoresis PAGE | 第30页 |
| ·Separation of proteins by PAGE | 第30页 |
| ·Wesrern analysis | 第30-31页 |
| ·Immunoblotting | 第31页 |
| ·Extraction of total RNA from plants | 第31-32页 |
| ·Quantitative RT-PCR analyses | 第32-33页 |
| ·Statistical analysis | 第33页 |
| ·Database addresses and software used | 第33-34页 |
| CHAPTER Ⅲ Light enhances seedling salt resistance | 第34-40页 |
| ·Introduction | 第34-35页 |
| ·Results | 第35-40页 |
| ·High light intensity improves frequency of seed germination | 第35-37页 |
| ·Light intensity contributes to root growth under salt stress | 第37-39页 |
| ·Light Intensity helps plant survival under salt stress | 第39-40页 |
| CHAPTER Ⅳ DELLA accumulation in response to light and NaCl increases seedling saltresistance | 第40-49页 |
| ·Introduction | 第40-41页 |
| ·Results | 第41-49页 |
| ·Light promotes seedling DELLA protein accumulation | 第41-42页 |
| ·Salt stress promote DELLAaccumulation | 第42-44页 |
| ·Light helps plant salt resistance by promoting DELLA protein accumulation | 第44-48页 |
| ·Light dependent germination frequency in Arabidopsis DELLA mutants | 第44-45页 |
| ·Light increases lateral root growth under salt stress | 第45-47页 |
| ·Light helps plant salt survival through DELLA | 第47-48页 |
| ·Hypocotyl length is in proportionate with DELLA accumulation | 第48-49页 |
| CHAPTER Ⅴ Expression of salt-responsive genes in DELLA mutant | 第49-53页 |
| ·Introduction | 第49-50页 |
| ·Results | 第50-53页 |
| ·DELLA dependent salt-responsive genes expression in gai mutant | 第50-51页 |
| ·Expression of salt-responsive genes in response to high light | 第51页 |
| ·Expression of salt-responsive genes under salt stress | 第51-53页 |
| DISCUSSION | 第53-59页 |
| CONCLUSION | 第59-60页 |
| REFERENCES | 第60-71页 |
| SUPPLEMENTARY DATA | 第71-74页 |
| ACKNOWLEDGEMENTS | 第74-75页 |
| CURRICULUM VITAE | 第75页 |
