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Characterizing Microbial Activity and Humic Substances of Red Soils under Long-Term Fertilization in Southern China

ABSTRACT第9-12页
摘要第13-16页
Chapter 1 Advances in the impacts of agricultural management practices on soilquality/healt第16-56页
    1.1. Soil quality/health and indicators第16-24页
    1.2. Effects of agricultural management practices on soil quality第24-31页
        1.2.1. Cultivation/tillage第25-27页
        1.2.2. Crop rotation第27-28页
        1.2.3. Inorganic and organic amendments第28-31页
        1.2.4. Pesticides第31页
    1.3. Microorganisms in soils第31-36页
        1.3.1. Significance of soil microorganisms第31-33页
        1.3.2. Impacts of agricultural practices on soil microorganisms第33-36页
    1.4. Enzymes activities in soils第36-47页
        1.4.1. Importance of soil enzymes第36-39页
        1.4.2. Impact of agricultural management practices on soil enzyme activities第39-47页
    1.5. Soil humic substances第47-51页
        1.5.1. Roles of Humic substances (HS)第47页
        1.5.2. Influence of agricultural practices on the composition and structure of HS第47-51页
    1.6. Research gaps, hypotheses, aims, and structure of the thesis第51-56页
Chapter 2 Assessment of microbial activities in the long-term fertilizationexperimental soils第56-84页
    2.1. Site description, Experimental design and Basic soil properties第56-63页
    2.2. Statistical analyses第63-64页
    2.3. Microcalorimetric measurements of microbial activity第64-70页
        2.3.1. Introduction第64页
        2.3.2. Description of the method第64-65页
        2.3.3. Results第65页
        2.3.4. Discussion第65-67页
        2.3.5. Conclusions/recommendations第67-70页
    2.4. Determination of soil microbial biomass carbon and nitrogen第70-73页
        2.4.1. Introduction第70页
        2.4.2. Description of the method第70-71页
        2.4.3. Results and discussion第71-72页
        2.4.4. Conclusions and recommendations第72-73页
    2.5. Measurement of enzyme activities in soils第73-84页
        2.5.1. Introduction第73-75页
        2.5.2. Description of the methods第75-79页
            -Dehydrogenase第75-76页
            -phosphatase第76-77页
            -Urease第77-78页
            -Casein-protease第78页
            -Invertase第78-79页
            -Catalase第79页
        2.5.3. Results and Discussion第79-81页
        2.5.4. Conclusions and recommendations第81-84页
Chapter 3 Characterization of soil microbial communities第84-94页
    3.1. Introduction第84页
    3.2. Description of the methods第84-89页
        3.2.1. Isolation and identification of total soil microorganisms第84-85页
        3.2.2. Isolation and identification of specific bacteria第85-86页
            -Denitrifying bacteria第85页
            -Aerobic N2-fixing bacteria (Azotobacter)第85-86页
            -Aerobic cellulose-decomposing bacteria (Plate counting method)第86页
        3.2.3. Extraction of soil DNA第86-87页
        3.2.4. PCR-DGGE第87-89页
    3.3. Results and Discussion第89-90页
    3.4. Conclusions and recommendations第90-94页
Chapter 4 Elemental and optical analyses of humic substances第94-101页
    4.1. Introduction第94页
    4.2. Materials and Methods第94-95页
    4.3. Results and Discussion第95-98页
    4.4. Conclusions第98-101页
Chapter 5 Spectroscopic characteristics of humic substances in long-termfertilization experimental soils第101-107页
    5.1. FTIR-spectroscopy of humic substances第101-107页
        5.1.1. Introduction第101页
        5.1.2. Material and Methods第101-102页
            -Extractions and separation of humic fractions第101-102页
            -Sample preparation and spectra collection第102页
        5.1.3. Results and Discussion第102-104页
        5.1.4. Conclusions第104-107页
Chapter 6 Concluding remarks and Perspectives第107-110页
References第110-130页
ACKNOWLEDGEMENTS第130-132页
LIST OF PUBLICATIONS第132-133页
Appendix第133-145页

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