| 摘要 | 第1-5页 |
| Abstract | 第5-9页 |
| Chapter 1 Introduction | 第9-24页 |
| ·What is Acid Mine Drainage(AMD) | 第9页 |
| ·The occurrence and formation of AMD | 第9-12页 |
| ·Chemical process during the formation of AMD | 第10-11页 |
| ·Biological processes contribute to the formation of AMD | 第11-12页 |
| ·The effects of AMD | 第12-13页 |
| ·The effects of AMD on acidity of the environment | 第12页 |
| ·The production of Iron(Ⅲ) hydroxide and potentially toxic metal ions | 第12-13页 |
| ·Microorganisms in AMD | 第13-15页 |
| ·The microbial diversity in AMD is remarkable | 第13页 |
| ·Physiology of and energy metabolism of microbes in AMD | 第13-15页 |
| ·Acidithiobacillus ferrooxidans:dominant resident and important function carrier in AMD | 第15-19页 |
| ·Basic information about Acidithiobacillus ferrooxidans | 第15-17页 |
| ·Iron metabolism of Acidithiobacillus ferrooxidans | 第17-19页 |
| ·Diversity in the A.ferrooxidans species | 第19-24页 |
| ·Microbial diversity—the key question of microbial ecology | 第19-21页 |
| ·Massive sequencing technology provides new insights into microbial diversity | 第21-22页 |
| ·About this paper:purpose and strategy | 第22-24页 |
| Chapter 2 Experimental | 第24-31页 |
| ·Bacteria isolation and characterization | 第25-26页 |
| ·Extraction and quality check of bacteria genomic DNA | 第26-27页 |
| ·Amplification and RFLP analysis of 16S rRNA gene and gyrB gene | 第27-28页 |
| ·Functional gene selection,primer design,amplification and sequencing | 第28-29页 |
| ·Whole genome based repetitive sequence based polymerase chain reaction | 第29-31页 |
| Chapter 3 Results and discussion | 第31-47页 |
| ·Bacteria isolation | 第31-32页 |
| ·Physiology characterization of isolates | 第32-33页 |
| ·Iron oxidation capability measurement | 第33-34页 |
| ·Quality check of bacteria genomic DNA | 第34-35页 |
| ·PCR-RFLP of 16S rRNA gene and gyrB gene and phylogenetic identification of isolates | 第35-37页 |
| ·PCR-RFLP result | 第35-37页 |
| ·Diversity analysis and phylogenetic identification of all isolates | 第37页 |
| ·Sequencing and modeling of functional genes | 第37-41页 |
| ·Amplification of three functional genes | 第37-39页 |
| ·Sequence comparison and stereo modeling of gene transcript products | 第39-41页 |
| ·Comparative rep-PCR analysis of isolates and other 30 strains of Leptospirillum ferriphilium | 第41-47页 |
| ·Gel electrophoresis results | 第41-42页 |
| ·Reproducibility check and data processing | 第42-43页 |
| ·Comparison of BOX-PCR and ERIC-PCR results | 第43-47页 |
| Chapter 4 Discussion | 第47-51页 |
| ·16S rRNA gene and gyrB gene reveal limited diversity in A.ferrooxidans | 第47-48页 |
| ·Three functional genes were highly conserved | 第48-49页 |
| ·rep-PCR revealed more divergence of A.ferrooxidans strains | 第49-51页 |
| Chapter 5 Conclusion | 第51-53页 |
| References | 第53-61页 |
| Acknowledgement | 第61-62页 |
| Papers published and accepted during graduate study | 第62页 |
