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Control Aggregation of Gold Nanoparticles for Colorimetric Assay of Biotin and Streptavidin

Abstract第5-6页
摘要第7-10页
Chapter 1 Introduction第10-33页
    1- Gold nanoparticles第12-18页
        1-1 Background第12-13页
        1-2 Surface Plasmon Resonance of gold nanoparticles第13-14页
        1-3 Gold nanoparticles aggragation第14-15页
        1-4 Synthesis of gold nanoparticles第15-18页
    2- Biotin第18-19页
    3- Streptavidin第19页
    4- Biotin-Streptavidin Affinity System第19-22页
    5- 2- [(4-hydroxyphenyl)azo] benzoic acid (HABA)第22-23页
    6- Application of gold nanopartiles for colorimetric detection第23-29页
    7- UV-visible spectroscopy第29-31页
    8- Transmission electron microscope第31-33页
Chapter 2 Colorimetric detection of biotin based on streptavidin-functionalized gold nanoparticles第33-43页
    2-1 background第33-34页
    2-2 Experimental Section第34-36页
        2-2-1 Materials第34页
        2-2-2 Characterization Techniques第34页
        2-2-3 Prepration of gold nanoparticles第34-35页
        2.2.4 Conjugation of streptavidin to gold nanoparticles第35页
        2.2.5 Optimation of concentration of STV for conjugation of streptavidin to gold nanoparticles第35-36页
        2.2.6 Conjugation of streptavidin to gold nanoparticles with different buffer solutions第36页
        2.2.7 Detection of biotin第36页
    2.3 Results and dscussion第36-43页
Chapter 3 Colorimetric detection of streptavidin using organic dye-functionalized gold nanoparticles第43-50页
    3.1 Background第43-44页
    3.2 Experimental Section第44-46页
        3.2.1 Materials第44页
        3.2.2 Characterization Techniques第44-45页
        3.2.3 Prepration of gold nanoparticles第45页
        3.2.4 Rapid Synthesis of Thiol-ssDNA-HABA-Functionalized Gold Nanoparticles第45页
        3.2.5 Detection of streptavidin第45-46页
    3.3 Results and discussion第46-50页
Conclusion第50-52页
References第52-55页
Acknowledgements第55页

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