| Abstract | 第1-8页 |
| 摘要 | 第8-10页 |
| Abbreviations | 第10-12页 |
| Chapter Ⅰ Literature review | 第12-25页 |
| ·Introduction | 第12页 |
| ·Description of somatic cell nuclear transfer technology | 第12-13页 |
| ·The procedure of somatic cell nuclear transfer technology | 第13-19页 |
| ·Epigenetics overview | 第19-21页 |
| ·Trichostatin A and its effect | 第21-23页 |
| ·The meaning and purpose of this test | 第23-25页 |
| Chapter Ⅱ Materials and Methods | 第25-37页 |
| ·Instruments and materials | 第25-28页 |
| ·Test procedure and methods | 第28-33页 |
| ·Experiment Design | 第33-35页 |
| ·Somatic cell counting method | 第35-36页 |
| ·Calculated in the test | 第36页 |
| ·Statistical Analysis | 第36-37页 |
| Chapter Ⅲ Results and analysis | 第37-44页 |
| ·Effect of TSA on the morphology of the somatic cells in Yanbian yellow cattle | 第37-41页 |
| ·The effect of different TSA concentrations treated somatic cells on the development of reconstructed embryos | 第41-42页 |
| ·The effect of donor cells with different processing time on the development of reconstructed embryos | 第42页 |
| ·The effect of co-cultured TSA treated somatic cells on the development of nuclear transfer cleavaged embryos | 第42-44页 |
| Chapter Ⅳ Discussion | 第44-47页 |
| ·The effect of TSA on the morphology and proliferation characteristics of somatic cells in Yanbian yellow cattle | 第44-45页 |
| ·The effect of somatic cells with different TSA treatment concentrations on the development of reconstructed embryos | 第45页 |
| ·The effect of donor cells with different processing time on the development of reconstructed embryos | 第45-46页 |
| ·The effect of co-cultured TSA treated somatic cells on the development of nuclear transfer cleavaged embryos | 第46-47页 |
| Chapter Ⅴ Conclusion | 第47-48页 |
| Reference | 第48-53页 |
| Acknowledgements | 第53页 |
