摘要 | 第6-8页 |
ABSTRACT | 第8-9页 |
LIST OF ABBREVIATIONS | 第13-21页 |
CHAPTER 1 GENERAL INTRODUCTION | 第21-46页 |
1.1 RESEARCH BACKGROUND | 第21-24页 |
1.2 LITERATURE REVIEW | 第24-44页 |
1.2.1 Trichoderma | 第24页 |
1.2.2 Taxonomy of Trichoderma | 第24-27页 |
1.2.3 Morphology of Trichoderma | 第27-31页 |
1.2.4 Identification of species based on the analysis of DNA sequences | 第31-36页 |
1.2.5 Internal Transcribed Spacer (ITS) | 第36页 |
1.2.6 Roles of Trichoderma as Biological Control Agent | 第36-42页 |
1.2.7 Trichoderma-plant Interactions | 第42-44页 |
1.3 RESEARCH AIM AND OBJECTIVES | 第44-45页 |
1.3.1 Aim | 第44-45页 |
1.4 FLOW CHART OF OUR STUDY | 第45-46页 |
CHAPTER 2 BIODIVERSITY OF THE GENUS HYPOCREA / TRICHODERMA IN DIFFERENT GRASSLAND AND FOREST SOIL ECOSYSTEMS OF INNER MONGOLIA, NORTHERN CHINA | 第46-118页 |
2.1 INTRODUCTION | 第46-47页 |
2.2 MATERIALS AND METHODS | 第47-55页 |
2.2.1 Sample sites and sampling procedure | 第47-49页 |
2.2.2 Isolation of Trichoderma spp | 第49页 |
2.2.3 Stock Culture | 第49-50页 |
2.2.4 Morphological Characterization and Growth Rate | 第50-51页 |
2.2.5 Molecular Analysis | 第51-55页 |
2.3 RESULTS | 第55-114页 |
2.3.1 Species identification | 第55-57页 |
2.3.2 Morphological Characterization | 第57页 |
2.3.3 Growth rate | 第57-85页 |
2.3.4 Molecular Analysis | 第85-86页 |
2.3.5 Phylogenetic analysis | 第86-94页 |
2.3.6 New species Descriptions | 第94-107页 |
2.3.7 Diversity of Trichoderma/ Hypocrea isolates in Inner Mongolia | 第107-114页 |
2.4 DISCUSSION | 第114-118页 |
CHAPTER 3 DIVERSITY OF TRICHODERMA ISOLATES FROM INNER MONGOLIA SOIL ON BIOLOGICAL CONTROL POTENTIAL | 第118-149页 |
3.1 INTRODUCTION | 第118-119页 |
3.2 MATERIALS AND METHODS | 第119-123页 |
3.2.1 Trichoderma Strains Used | 第120页 |
3.2.2 Collection of pathogens | 第120页 |
3.2.3 In vitro antagonistic tests | 第120-121页 |
3.2.4 Evaluation on biocontrol and plant growth promotion in vivo | 第121-123页 |
3.2.5 Statistical analysis | 第123页 |
3.3 RESULT | 第123-145页 |
3.3.1 In vitro Screening for Antagonistic Potential of Twenty-one Species of Trichoderma against Different Plant Pathogenic Fungi | 第123-126页 |
3.3.2 Screening of indigenous potential antagonistic Trichoderma strains from Inner Mongolia soil against selected phytopathogenic fungi | 第126-129页 |
3.3.3 Analysis of the Antagonistic effect for T.cf harzianum on selected phytopathogenic fungi | 第129-135页 |
3.3.4 Biocontrol efficacy of Trichoderma spp. against wilt of cucumber caused by Fusarium oxysporum f.sp. cucumerinum under In vivo conditions | 第135-139页 |
3.3.5 Effect of treating cucumber seeds with Trichoderma isolates on the severity of Fusarium wilt disease | 第139-142页 |
3.3.6 Biocontrol Potential of Local Trichoderma isolates from Inner Mongolia based on their isolation regions and ecosystem | 第142-143页 |
3.3.7 Biocontrol efficacy of T.cf. harzianum against wilt disease of Cucumber caused by F. oxysporum f.sp. cucumerinum | 第143-145页 |
3.4 DISCUSSION | 第145-149页 |
3.4.1 Biocontrol potential of the isolated strains | 第145-149页 |
CHAPTER 4 DIVERSITY OF LYTIC ENZYMATIC ACTION OF TRICHODERMA ISOLATES SELECTED BASED ON BIOLOGICAL CONTROL PERFORMANCE | 第149-172页 |
4.1 INTRODUCTION | 第149-150页 |
4.2 MATERIALS AND METHODS | 第150-153页 |
4.2.1 Qualitative screening of Extra Cellular Enzyme | 第150-151页 |
4.2.2 Quantitative estimations of Extra Cellular Enzyme | 第151-153页 |
4.3 RESULT | 第153-165页 |
4.3.1 In vitro antifungal activity | 第154-155页 |
4.3.2 Determination of different biocontrol enzymatic activity | 第155-161页 |
4.3.3 Spectrophotometric determination of Enzyme activity of Trichoderma isolates | 第161-165页 |
4.4 DISCUSSIONS | 第165-172页 |
CHAPTER 5 OVERALL CONCLUSION AND RECOMMENDATIONS | 第172-177页 |
5.1 CONCLUSION | 第172-176页 |
5.2 RECOMMENDATION | 第176-177页 |
ACKNOWLEDGEMENTS | 第177-178页 |
RESUME | 第178-179页 |
Publications | 第179-180页 |
REFERENCES | 第180-199页 |
Appendix | 第199-231页 |