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内蒙古草原和森林生态系统木霉菌生物多样性研究

摘要第6-8页
ABSTRACT第8-9页
LIST OF ABBREVIATIONS第13-21页
CHAPTER 1 GENERAL INTRODUCTION第21-46页
    1.1 RESEARCH BACKGROUND第21-24页
    1.2 LITERATURE REVIEW第24-44页
        1.2.1 Trichoderma第24页
        1.2.2 Taxonomy of Trichoderma第24-27页
        1.2.3 Morphology of Trichoderma第27-31页
        1.2.4 Identification of species based on the analysis of DNA sequences第31-36页
        1.2.5 Internal Transcribed Spacer (ITS)第36页
        1.2.6 Roles of Trichoderma as Biological Control Agent第36-42页
        1.2.7 Trichoderma-plant Interactions第42-44页
    1.3 RESEARCH AIM AND OBJECTIVES第44-45页
        1.3.1 Aim第44-45页
    1.4 FLOW CHART OF OUR STUDY第45-46页
CHAPTER 2 BIODIVERSITY OF THE GENUS HYPOCREA / TRICHODERMA IN DIFFERENT GRASSLAND AND FOREST SOIL ECOSYSTEMS OF INNER MONGOLIA, NORTHERN CHINA第46-118页
    2.1 INTRODUCTION第46-47页
    2.2 MATERIALS AND METHODS第47-55页
        2.2.1 Sample sites and sampling procedure第47-49页
        2.2.2 Isolation of Trichoderma spp第49页
        2.2.3 Stock Culture第49-50页
        2.2.4 Morphological Characterization and Growth Rate第50-51页
        2.2.5 Molecular Analysis第51-55页
    2.3 RESULTS第55-114页
        2.3.1 Species identification第55-57页
        2.3.2 Morphological Characterization第57页
        2.3.3 Growth rate第57-85页
        2.3.4 Molecular Analysis第85-86页
        2.3.5 Phylogenetic analysis第86-94页
        2.3.6 New species Descriptions第94-107页
        2.3.7 Diversity of Trichoderma/ Hypocrea isolates in Inner Mongolia第107-114页
    2.4 DISCUSSION第114-118页
CHAPTER 3 DIVERSITY OF TRICHODERMA ISOLATES FROM INNER MONGOLIA SOIL ON BIOLOGICAL CONTROL POTENTIAL第118-149页
    3.1 INTRODUCTION第118-119页
    3.2 MATERIALS AND METHODS第119-123页
        3.2.1 Trichoderma Strains Used第120页
        3.2.2 Collection of pathogens第120页
        3.2.3 In vitro antagonistic tests第120-121页
        3.2.4 Evaluation on biocontrol and plant growth promotion in vivo第121-123页
        3.2.5 Statistical analysis第123页
    3.3 RESULT第123-145页
        3.3.1 In vitro Screening for Antagonistic Potential of Twenty-one Species of Trichoderma against Different Plant Pathogenic Fungi第123-126页
        3.3.2 Screening of indigenous potential antagonistic Trichoderma strains from Inner Mongolia soil against selected phytopathogenic fungi第126-129页
        3.3.3 Analysis of the Antagonistic effect for T.cf harzianum on selected phytopathogenic fungi第129-135页
        3.3.4 Biocontrol efficacy of Trichoderma spp. against wilt of cucumber caused by Fusarium oxysporum f.sp. cucumerinum under In vivo conditions第135-139页
        3.3.5 Effect of treating cucumber seeds with Trichoderma isolates on the severity of Fusarium wilt disease第139-142页
        3.3.6 Biocontrol Potential of Local Trichoderma isolates from Inner Mongolia based on their isolation regions and ecosystem第142-143页
        3.3.7 Biocontrol efficacy of T.cf. harzianum against wilt disease of Cucumber caused by F. oxysporum f.sp. cucumerinum第143-145页
    3.4 DISCUSSION第145-149页
        3.4.1 Biocontrol potential of the isolated strains第145-149页
CHAPTER 4 DIVERSITY OF LYTIC ENZYMATIC ACTION OF TRICHODERMA ISOLATES SELECTED BASED ON BIOLOGICAL CONTROL PERFORMANCE第149-172页
    4.1 INTRODUCTION第149-150页
    4.2 MATERIALS AND METHODS第150-153页
        4.2.1 Qualitative screening of Extra Cellular Enzyme第150-151页
        4.2.2 Quantitative estimations of Extra Cellular Enzyme第151-153页
    4.3 RESULT第153-165页
        4.3.1 In vitro antifungal activity第154-155页
        4.3.2 Determination of different biocontrol enzymatic activity第155-161页
        4.3.3 Spectrophotometric determination of Enzyme activity of Trichoderma isolates第161-165页
    4.4 DISCUSSIONS第165-172页
CHAPTER 5 OVERALL CONCLUSION AND RECOMMENDATIONS第172-177页
    5.1 CONCLUSION第172-176页
    5.2 RECOMMENDATION第176-177页
ACKNOWLEDGEMENTS第177-178页
RESUME第178-179页
Publications第179-180页
REFERENCES第180-199页
Appendix第199-231页

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