| Abstract | 第6页 |
| 摘要 | 第7-12页 |
| List of abbreviations | 第12-14页 |
| Chapter One: Background | 第14-28页 |
| 1.1 Introduction | 第14-15页 |
| 1.2 Occurrence of PAEs | 第15-22页 |
| 1.2.1 Occurrence in surface waters and sediments | 第16-18页 |
| 1.2.2 Occurrence in Air | 第18-20页 |
| 1.2.3 Occurrence in soils | 第20-21页 |
| 1.2.4 Occurrence in wastewater treatment plants and sludge | 第21页 |
| 1.2.5 Occurrence in landfills | 第21-22页 |
| 1.3 Eco-toxicological effects of PAEs | 第22-25页 |
| 1.3.1 Effect on aquatic organisms | 第23页 |
| 1.3.2 Effects on terrestrial organisms | 第23-24页 |
| 1.3.3 Effect on humans | 第24-25页 |
| 1.4 PAEs removal methods | 第25-27页 |
| 1.4.1 Physical and chemical methods of PAEs removal | 第25页 |
| 1.4.2 Biodegradation of PAEs | 第25-27页 |
| 1.5 Aim and objectives | 第27-28页 |
| 1.5.1 General objective | 第27页 |
| 1.5.2 Specific objectives | 第27-28页 |
| Chapter Two: Isolation and characterization of a bacterium capable of degrading PAEs | 第28-43页 |
| 2.1 Materials and methods | 第28-31页 |
| 2.1.1 Reagents and media | 第28页 |
| 2.1.2 Enrichment and isolation of DEHP degrading bacterial strain | 第28-29页 |
| 2.1.3 Microbial identification and phylogenetic analysis | 第29-30页 |
| 2.1.4 Effect of environmental factors on DEHP degradation by strain YC-RL2 | 第30页 |
| 2.1.5 Substrate utilization tests | 第30-31页 |
| 2.1.6 Analytical methods | 第31页 |
| 2.2 Results and discussion | 第31-43页 |
| 2.2.1 Isolation and identification of the DEHP degrading bacterium | 第31-33页 |
| 2.2.2 Effect of temperature on DEHP degradation | 第33-36页 |
| 2.2.3 Effect of p H | 第36-37页 |
| 2.2.4 Effect of Na Cl concentration | 第37-39页 |
| 2.2.5 Substrate utilization tests | 第39-41页 |
| 2.2.6 Effect of initial DEHP concentration | 第41-42页 |
| 2.2.7 Accession numbers | 第42-43页 |
| Chapter Three: Identification of DEHP degradation intermediates and cloning of genes involved in PAEdegradation | 第43-52页 |
| 3.1 Materials and methods | 第43-44页 |
| 3.1.1 Detection of DEHP degradation intermediates by HPLC-MS | 第43页 |
| 3.1.2 Cloning and expression of mehp H gene in E.coli cells | 第43-44页 |
| 3.2 Results and discussion | 第44-52页 |
| 3.2.1 DEHP degradation intermediates and proposed degradation pathway | 第44-49页 |
| 3.2.2 Cloning and expression of mehp H gene | 第49-52页 |
| Chapter Four: Application potential of strain YC-RL2 in remediating PAEs contaminated soil | 第52-55页 |
| 4.1 Materials and methods | 第52页 |
| 4.2 Results and discussion | 第52-55页 |
| Chapter Five: General conclusions | 第55-56页 |
| References | 第56-77页 |
| Acknowledgements | 第77-78页 |
| Appendices | 第78-80页 |
