| Acknowledgements | 第11-14页 |
| Table of contents | 第14-18页 |
| List of Tables | 第18-19页 |
| List of figures | 第19-20页 |
| Abbreviations | 第20-23页 |
| Abstract | 第23-24页 |
| 摘要 | 第25-27页 |
| Chapter 1 Introduction | 第27-31页 |
| Chapter 2 Literature review | 第31-55页 |
| 2.1 Agaricus bisporus | 第31-32页 |
| 2.2 Classification of button mushroom(Agaricus bisporus) | 第32页 |
| 2.3 Mushroom cell wall composition,their degrading enzymes and genes | 第32-33页 |
| 2.4 Chitin,chitinase and their encoding genes | 第33-35页 |
| 2.5 Cellulose,cellulase and their encoding gene | 第35-36页 |
| 2.6 Glucans and Glucanase enzymes,and their encoding genes | 第36-37页 |
| 2.7 Phenylalanine ammonialyase(PAL) | 第37-38页 |
| 2.8 Malondialdehyde(MDA) | 第38-39页 |
| 2.9 Reactive oxygen species(ROS) | 第39-41页 |
| 2.10 Antioxidant machinery | 第41-42页 |
| 2.11 Approaches for prolonging the shelf-life of mushrooms | 第42页 |
| 2.12 Pre-harvest approaches | 第42-44页 |
| 2.12.1 Calcium salts treatment before harvesting | 第43页 |
| 2.12.2 Calcium salts and chlorine dioxide(Oxine)treatment before harvesting | 第43-44页 |
| 2.12.3 Addition of H_2O_2 and CaCl_2 to the irrigated water | 第44页 |
| 2.12.4 Control of bacterial yellowing of mushroom using acetic or hydrochloric acidsolutions | 第44页 |
| 2.13 Post-harvest processing for quality enhancement of mushrooms | 第44-55页 |
| 2.13.1 Controlled atmosphere(CA)storage | 第45-46页 |
| 2.13.2 Washing procedures | 第46-47页 |
| 2.13.3 Ozone treatment | 第47页 |
| 2.13.4 Irradiation treatment | 第47-49页 |
| 2.13.5 Use of edible coatings | 第49-50页 |
| 2.13.6 Button mushroom quality maintainer humectants | 第50-51页 |
| 2.13.7 Use of composite chemical treatment for button mushroom preservation | 第51-55页 |
| Chapter 3 Effects of composite chemical treatments on quality of button mushroom (Agaricus bisporus) | 第55-63页 |
| 3.1. Introduction | 第55-56页 |
| 3.2. Materials and Methods | 第56-57页 |
| 3.2.1 Mushrooms and composite chemical pretreatment | 第56页 |
| 3.2.2. Measurement of weight | 第56-57页 |
| 3.2.3. Measurement of texture(Firmness) | 第57页 |
| 3.2.4. Measurement of color(L~* values) | 第57页 |
| 3.2.5 Statistical analysis | 第57页 |
| 3.3. Results and discussion | 第57-63页 |
| 3.3.1 Effect of composite chemical pretreatment on weight | 第57-58页 |
| 3.3.2 Effect of composite chemical pretreatment on texture | 第58-60页 |
| 3.3.3 Effect of composite chemical pretreatment on color | 第60-61页 |
| 3.3.4 Effect of composite chemical pre-treatment on total soluble protein contents | 第61-63页 |
| Chapter 4 Effects of composite chemical pretreatments on antioxidants enzymes and reactive oxygen species | 第63-75页 |
| 4.1 Introduction | 第63-64页 |
| 4.2 Materials and methods | 第64-66页 |
| 4.2.2 Sample preparation,treatment and storage | 第64页 |
| 4.2.3 Measurement of lipid peroxidation and reactive oxygen species(ROS) | 第64-65页 |
| 4.2.4 Measurement of antioxidant enzymes | 第65-66页 |
| 4.2.5 Statistical analysis | 第66页 |
| 4.3 Results and discussion | 第66-75页 |
| 4.3.1 Effect of composite chemical pretreatment on lipid peroxidation | 第66-67页 |
| 4.3.2 Effect of composite chemical pretreatment on reactive oxygen species(ROS) | 第67-70页 |
| 4.3.3 Effect of composite chemical pretreatment on antioxidant enzymes and total solubleprotein contents | 第70-75页 |
| Chapter 5 Effect of Postharvest chemical treatment on texture and cell wall secondary metabolism of button mushroom(Agaricus bisporus) | 第75-90页 |
| 5.1 Introduction | 第76-77页 |
| 5.2. Materials and methods | 第77-82页 |
| 5.2.1. Samples preparation,treatment and storage | 第77-78页 |
| 5.2.2. Weight,color and texture | 第78页 |
| 5.2.3. Composition of cell wall | 第78-79页 |
| 5.2.3.1. Determination of cellulose | 第78-79页 |
| 5.2.3.2. Determination of chitin | 第79页 |
| 5.2.4. Extraction of enzymes and their activities measurement | 第79-80页 |
| 5.2.5. RNA extraction,cDNA preparation and qPCR | 第80-82页 |
| 5.3. Results | 第82-86页 |
| 5.3.1. Variations in physical characteristics (Weight, color and texture) | 第82-83页 |
| 5.3.2. Cell wall composition (Cellulose and Chitin) | 第83-84页 |
| 5.3.3. Cell wall degrading enzymes activities (Cellulase,β-1,3 Glucanase, Chitinase,phenylalanine ammonialyase) | 第84-85页 |
| 5.3.4. Gene expression of cell wall degrading enzymes | 第85-86页 |
| 5.4. Discussion | 第86-90页 |
| Chapter 6 Major conclusions and future perspectives | 第90-93页 |
| 6.1 Major conclusions | 第90-91页 |
| 6.2. Future challenges and recommendations | 第91-93页 |
| References | 第93-109页 |
| List of publications | 第109-111页 |
