| Abstract | 第13-14页 |
| 中文摘要 | 第15-30页 |
| Chapter 1:Introduction | 第30-55页 |
| 1.1 Mass spectrometry-based proteomics | 第30-45页 |
| 1.1.1 The concept of proteomics | 第30页 |
| 1.1.2 Mass spectrometry basics | 第30-33页 |
| 1.1.3 Mass spectrometry-based proteomics technique | 第33-36页 |
| 1.1.4 Quantitative proteomics | 第36-43页 |
| 1.1.5 Data analysis and bioinformatics tool | 第43-45页 |
| 1.2 Salmonella pathogenesis | 第45-54页 |
| 1.2.1 Salmonella overview | 第45-49页 |
| 1.2.2 Two-component system | 第49-53页 |
| 1.2.3 The application of proteomics in Salmonella pathogenesis | 第53-54页 |
| 1.3 Research hypothesis and aims | 第54-55页 |
| Chapter 2:Proteomics Analysis of RstA/RstB System in Salmonella Typhimurium | 第55-95页 |
| 2.1 Introduction | 第55-56页 |
| 2.2 Materials and methods | 第56-72页 |
| 2.2.1 Bacterial strains,medium and growth conditions | 第56-58页 |
| 2.2.2 Protein sample preparation | 第58-59页 |
| 2.2.3 Two-dimensional electrophoresis methods | 第59-61页 |
| 2.2.4 Protein digestion | 第61-64页 |
| 2.2.5 Peptide stable isotope dimethyl labeling | 第64-65页 |
| 2.2.6 Peptide separation by strong cation exchange(SCX)chromatography | 第65-66页 |
| 2.2.7 Mass spectrometry analysis and data processing | 第66-68页 |
| 2.2.8 Bioinformatics analysis | 第68页 |
| 2.2.9 Quantitative real-time PCR(qPCR)analysis | 第68-70页 |
| 2.2.10 Phenotype analysis | 第70-72页 |
| 2.3 Results and discussion | 第72-93页 |
| 2.3.1 Experimental strategies and proteomics workflow | 第72-73页 |
| 2.3.2 Overview of the proteomics data | 第73-75页 |
| 2.3.3 Metabolic pathways and function groups regulated by RstA/RstB | 第75-81页 |
| 2.3.4 RstA/RstB TCS regulation of pyrimidine metabolism | 第81-85页 |
| 2.3.5 RstA/RstB TCS regulation of iron acquisition | 第85-89页 |
| 2.3.6 RstA/RstB regulation of surface motility, chemotaxis and invasion | 第89-93页 |
| 2.4 Conclusion | 第93-95页 |
| Chapter 3:Proteomics Analysis of PhoP/PhoQ System in SalmonellaTyphimurium | 第95-115页 |
| 3.1. Introduction | 第95-96页 |
| 3.2. Materials and methods | 第96页 |
| 3.2.1. Bacterial strains, medium and growth conditions | 第96页 |
| 3.2.2. Proteins sample preparation | 第96页 |
| 3.2.3 Biofilm phenotype analysis | 第96页 |
| 3.3. Results and discussion | 第96-113页 |
| 3.3.1 Experimental strategies and proteomics workflow | 第96-98页 |
| 3.3.2 Overview of the proteomics data | 第98-100页 |
| 3.3.3 Metabolic pathways and function groups regulated by PhoP/PhoQ system | 第100-106页 |
| 3.3.4 Network analysis of proteins regulated by PhoP/PhoQ system | 第106-107页 |
| 3.3.5 PhoP/PhoQ regulates several two-component systems | 第107-109页 |
| 3.3.6 PhoP/PhoQ regulation of ribosome proteins | 第109-111页 |
| 3.3.7 PhoP/PhoQ regulates ABC transport proteins | 第111-112页 |
| 3.3.8 phoPQ mutant effected to biofilm formation | 第112-113页 |
| 3.4. Conclusion | 第113-115页 |
| Chapter 4:Comparative Proteomics Analysis of Salmonella Typhimurium underthe Regulation of RstA/RstB and PhoP/PhoQ Systems | 第115-127页 |
| 4.1 Introduction | 第115-116页 |
| 4.2 Materials and Methods | 第116页 |
| 4.3 Results and discussion | 第116-125页 |
| 4.3.1 Comparative proteomics data overview of the two mutant strains | 第116页 |
| 4.3.2 Comparison of metabolic pathways and functional groups significantly regulated by RstA/RstB and PhoP/PhoQ TCSs | 第116-122页 |
| 4.3.3 Comparison of common proteins regulated by RstA/RstB and PhoP/PhoQ TCSs | 第122-125页 |
| 4.4 Conclusion | 第125-127页 |
| Chapter 5:Summary and Future Perspective | 第127-129页 |
| References | 第129-137页 |
| Supplemental S1.80 metabolic pathways of △rstB and △phoPQ strains | 第137-139页 |
| Supplemental S2.Changed protein of △rstB strain | 第139-143页 |
| Supplemental S3.Changed protein of △phoPQ strain | 第143-152页 |
| Research Publications | 第152-153页 |
| Acknowledgements | 第153页 |
