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Fine Mapping a Major Boll Weight QTL and Fingerprint Studies in Upland Cotton Using SSRs

Abbreviations第7-8页
Abstract第8-9页
Section (A) Fine mapping a major boll weight QTL in upland cotton using SSRs第10-35页
    Chapter 01 Introduction第10-12页
    Chapter 02 Review of Literature第12-19页
        2.1. Molecular mapping in plants第12页
        2.2. Molecular markers第12-13页
        2.3. Mapping populations第13-14页
            2.3.1. F_2 population第13-14页
            2.3.2. Back cross (BC) population第14页
            2.3.3. Recombinant inbred line (RIL) population第14页
        2.4. Linkage map and QTLs第14-15页
        2.5. Bulk segregation analysis (BSA)第15页
        2.6. Sequential fine mapping strategy第15-16页
        2.7. Fine mapping plant complex traits第16-17页
        2.8. Yield and fiber QTLs mapped in cultivated cotton第17-19页
    Chapter 03 Materials and methods第19-25页
        3.1. Plant materials第19页
        3.2. Mapping population第19-20页
        3.3. Phenotypic evaluation第20页
        3.4. SSR assays第20-24页
            3.4.1. DNA extraction第20-21页
            3.4.2. Chemical recipes第21-22页
            3.4.3. DNA quantity and quality estimation第22-23页
            3.4.4. PCR thermo profile and amplification mixture第23页
            3.4.5. Screening and identification of trait-linked SSRs第23页
            3.4.6. DNA PAGE第23页
            3.4.7. Silver staining and band scoring第23-24页
        3.5. Identification of linked SSRs第24页
        3.6. Linkage analysis and QTL mapping第24-25页
    Chapter 04 Results第25-33页
        4.1. Evaluating trait variation第25-26页
        4.2. Polymorphism and bulk segregation analysis (BSA)第26-27页
        4.3. Linkage mapping and QTL analysis第27-30页
            4.3.1. Bulk segregation analysis (BSA) SSRs第27-28页
            4.3.2. Saturation by region-specific SSRs第28-29页
            4.3.3. Large F_(2-2013) population第29-30页
        4.4. Locating qBW12 on D-genome physical map第30-31页
        4.5. High resolution genetic map of qBW12第31-33页
    Chapter 05 Discussion第33-35页
        5.1. Chromosome D08:A gene rich region第33页
        5.2. Future strategies第33-35页
            5.2.1. Fine mapping other yield and fiber related QTLs on D08第33页
            5.2.2. Identification of completely linked SSRs for BW trait in F_(2-2013)第33-34页
            5.2.3. Expression analysis of candidate genes underlying qBW12第34-35页
Section (B) Selection of core SSR markers for fingerprinting upland cotton第35-50页
    Chapter 06 Manuscript第35-50页
        6.1 Introduction第35-37页
        6.2. Materials and methods第37-39页
            6.2.1 Plant materials第37页
            6.2.2. DNA extraction第37页
            6.2.3. SSR analysis第37-38页
            6.2.4. Constructing SSR fingerprints using potential core SSRs第38页
            6.2.5. Polymerase chain reaction (PCR) and electrophoresis第38页
            6.2.6. Data analysis第38-39页
        6.3. Results第39-46页
            6.3.1. SSR polymorphism and resolving power第39页
            6.3.2. Estimation of genetic diversity第39-40页
            6.3.3. Utilization efficiency of SSR fingerprints第40页
            6.3.4. Genetic relatedness revealed by genome-covered SSRs第40-43页
            6.3.5. Genetic relatedness revealed by potential core SSRs第43-45页
            6.3.6. Comparing genome-covered and potential core SSRs第45-46页
        6.4. Discussion第46-48页
        6.5. Conclusion第48-50页
Supplementary data第50-59页
References第59-68页
Publication第68-69页
Acknowledgement第69-70页

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