| ACKNOWLEDGEMENTS | 第1-5页 |
| DEDICATION | 第5-6页 |
| LIST OF FIGURES | 第6-7页 |
| LIST OF TABLES | 第7-8页 |
| LIST OF ABBREVIATIONS | 第8-9页 |
| ABSTRACT | 第9-11页 |
| 摘要 | 第11-13页 |
| TABLE OF CONTENTS | 第13-17页 |
| CHAPTER 1 INTRODUCTION AND LITERATURE REVIEW | 第17-32页 |
| ·GENERAL INTRODUCTION:CYCLODEXTRIN GLYCOSYLTRANSFERASEENZYMES AND CYCLODEXTRIN PRODUCTION | 第17-18页 |
| ·CYCLODEXTRIN GLYCOSYLTRANSFERASE ENZYMES | 第18-26页 |
| ·Starch as a Substrate for CGTase Production | 第18-20页 |
| ·Structure and Properties of Starch | 第18-19页 |
| ·Starch processing enzymes | 第19-20页 |
| ·Cyclodextrin Glycosyltransferase | 第20-26页 |
| ·Substrate Binding and Catalytic Properties | 第21页 |
| ·Three-Dimensional Structures | 第21-22页 |
| ·Catalytic Mechanism and Evolution of Product Specificity | 第22-24页 |
| ·Reaction Specificities | 第24-26页 |
| ·CYCLODEXTRINS(CDS) | 第26-30页 |
| ·Chemical Structure and Properties | 第27页 |
| ·Solubility of CDs | 第27-28页 |
| ·Use and Regulatory Status of CDs | 第28-29页 |
| ·CD Production | 第29-30页 |
| ·PROBLEM STATEMENT AND JUSTIFICATION OF RESEARCH | 第30-31页 |
| ·Problem Statement | 第30页 |
| ·Justification of Research | 第30-31页 |
| ·MAIN OBJECTIVE | 第31-32页 |
| ·Specific Objectives | 第31-32页 |
| CHAPTER 2 EFFECT OF MEDIA COMPOSITION ON PRODUCTION AND ACTIVITY OFCGTASE FROM BACILLUS LICHENIFORMIS SK 13.002 STRAIN AND ITSAPPLICATION FOR B-CYCLODEXTRIN PRODUCTION | 第32-57页 |
| ·INTRODUCTION | 第32-34页 |
| ·MATERIALS AND METHODS | 第34-38页 |
| ·Materials | 第34页 |
| ·Bacterial Strain Cultivation Conditions for CGTase Production | 第34-35页 |
| ·Effect of Initial pH on CGTase Production | 第35页 |
| ·Effects of Media Composition on CGTase Production | 第35页 |
| ·Time Course Production of CGTase | 第35页 |
| ·CGTase Activity Assays | 第35-37页 |
| ·Making of a β-CD Calibration Curve | 第35-36页 |
| ·Cyclization Activity | 第36-37页 |
| ·Hydrolysis Activity | 第37页 |
| ·CGTase Activities as a Function of Temperature and pH | 第37页 |
| ·CGTase Thermal Stability | 第37页 |
| ·Analysis of CGTase CD Production by HPLC | 第37-38页 |
| ·Statistical Analysis | 第38页 |
| ·RESULTS AND DISCUSSION | 第38-54页 |
| ·Bacterial Strain Growth and Effect of Initial pH on CGTase Production | 第38-39页 |
| ·Effect of Carbon Sources on CGTase Production | 第39-41页 |
| ·Effect of Nitrogen Sources on CGTase Production | 第41-42页 |
| ·Effect of Metal Ions on CGTase Production | 第42-44页 |
| ·Time Course Production of CGTase | 第44-47页 |
| ·Effect of pH on Cyclization and Hydrolysis Activities | 第47-48页 |
| ·Effect of Temperature on Cyclization and Hydrolysis Activities | 第48-49页 |
| ·CGTase Thermal Stability | 第49-52页 |
| ·Production of CDs | 第52-54页 |
| ·CONCLUSIONS | 第54-57页 |
| CHAPTER 3 PURIFICATION OF BACILLUS LICHENIFORMIS SK 13.002 CGTASEISOENZYMES | 第57-63页 |
| ·INTRODUCTION | 第57页 |
| ·MATERIALS AND METHODS | 第57-59页 |
| ·Materials | 第57页 |
| ·CGTase Concentration and Precipitation | 第57-58页 |
| ·Purification of CGTase | 第58页 |
| ·DEAE-Sepharose Ion-Exchange Chromatography | 第58页 |
| ·Superdex-75 Gel Filtration Chromatography | 第58页 |
| ·Molecular Weight and Protein Quantification | 第58-59页 |
| ·RESULTS AND DISCUSSION | 第59-62页 |
| ·Concentration and Purification of CGTase | 第59-61页 |
| ·Homogeneity and Molecular Weights of CGTase Isozymes | 第61-62页 |
| ·CONCLUSIONS | 第62-63页 |
| 4. GENERAL CONCLUSIONS AND RECOMMENDATIONS | 第63-66页 |
| ·GENERAL CONCLUSIONS | 第63-65页 |
| ·RECOMMENDATIONS | 第65-66页 |
| 5. LIST OF PUBLICATIONS | 第66-67页 |
| 6. REFERENCES | 第67-77页 |
