| abstract | 第5-6页 |
| 摘要 | 第7-11页 |
| Chapter 1 Study of Host-Parasite Interaction, a Review | 第11-22页 |
| 1 Introduction | 第11-12页 |
| 2 Behavioral study trends and hypothesis | 第12页 |
| 3 Tinbergen’s paradigm for behavior study | 第12-16页 |
| 3.1 Adaptive behavior manipulation | 第13-15页 |
| 3.1.1 Complexity | 第13-14页 |
| 3.1.2 Purposiveness of design | 第14页 |
| 3.1.3 Convergence | 第14页 |
| 3.1.4 Fitness effects | 第14-15页 |
| 3.2 Distinction with Non-adaptive behavior change | 第15-16页 |
| 4 Significance of adaptive manipulation strategy in ecology and evolution | 第16-17页 |
| 5 Cost of behavior manipulation | 第17-19页 |
| 5.1 Parasite as fitness bearer | 第18页 |
| 5.2 Host as fitness bearer | 第18-19页 |
| 6 Consequences of behavior manipulation strategy | 第19-22页 |
| 6.1 Loss or reduction of non-behavioral strategies | 第19页 |
| 6.2 Plastic or fixed strategy | 第19-20页 |
| 6.3 Local adaptation | 第20页 |
| 6.4 Progressive arms race | 第20-22页 |
| Chapter 2 Insights in to the temporal gene expression pattern of Lymantria dispar larvae during the baculovirus induced hyperactive stage | 第22-43页 |
| 1 Introduction | 第22-23页 |
| 2 Materials and methods | 第23-28页 |
| 2.1 Insects and virus | 第23-24页 |
| 2.2 Locomotion assay | 第24-25页 |
| 2.3 Virus infection and sample preparation for RNA-Seq | 第25-26页 |
| 2.4 RNA isolation cDNA library construction and Illumina sequencing | 第26页 |
| 2.5 De Novo transcriptome assembly, annotation and differential expression analysis | 第26-27页 |
| 2.6 Gene set enrichment analysis (GSEA) and visualization | 第27页 |
| 2.7 Validation by quantitative RT-PCR | 第27-28页 |
| 2.8 Data availability | 第28页 |
| 3 Results and discussions | 第28-42页 |
| 3.1 Locomotion assay | 第28-30页 |
| 3.2 Sequencing and de novo assembly of the L dispar transcriptome | 第30页 |
| 3.3 Differential expression analysis | 第30-32页 |
| 3.4 Functional Annotations and enrichment analysis | 第32-36页 |
| 3.5 GSEA and visualization | 第36-37页 |
| 3.6 Genes involved in Neuroactive ligand receptor interaction pathway | 第37-41页 |
| 3.7 Validation of the data by quantitative RT-PCR | 第41-42页 |
| 4 Conclusion | 第42-43页 |
| Chapter 3 Photo-transduction and circadian entrainments are the key pathways in the signaling mechanism for the baculovirus induced tree-top disease in the lepidopteran larvae | 第43-72页 |
| 1 Introduction | 第43-45页 |
| 2 Materials and methods | 第45-50页 |
| 2.1 Insects and virus | 第45页 |
| 2.2 Locomotion assay | 第45页 |
| 2.3 Sample preparation for RNA sequencing | 第45-46页 |
| 2.4 RNA isolation cDNA library construction and Illumina sequencing | 第46页 |
| 2.5 De Novo transcriptome assembly and functional annotation | 第46-47页 |
| 2.6 Differential Expression analysis | 第47页 |
| 2.7 Gene set enrichment analysis and visualization | 第47页 |
| 2.8 RNA-Seq validation with quantitative RT-PCR | 第47-48页 |
| 2.9 Sample preparation and analysis of circadian genes | 第48-49页 |
| 2.10 Directional light bio-assay | 第49页 |
| 2.11 Data availability | 第49-50页 |
| 3 Results | 第50-70页 |
| 3.1 Locomotion assay | 第50-51页 |
| 3.2 Sequencing and de novo assembly of the L dispar transcriptome | 第51-52页 |
| 3.3 Functional Annotation | 第52-53页 |
| 3.4 Differentially gene expression analysis | 第53-57页 |
| 3.5 GO, KEGG and KOG enrichment analysis | 第57-60页 |
| 3.6 Ranked GSEA and Visualization | 第60-64页 |
| 3.7 Validation by quantitative RT-qPCR | 第64-65页 |
| 3.8 Rhythm of differentially expressed Circadian genes | 第65-68页 |
| 3.9 Candidate DEGs involved in sensory perception | 第68-70页 |
| 3.10 Directed light bioassay | 第70页 |
| 4 Conclusion | 第70-72页 |
| Chapter 4 Larval response to different wavelength of lights during baculovirus induced tree top disease | 第72-79页 |
| 1 Introduction | 第72-73页 |
| 2 Materials and methods | 第73-75页 |
| 2.1 Insect larvae, virus and lights | 第73-74页 |
| 2.2 Behavioral assay 1: light from above | 第74-75页 |
| 2.3 Behavioral assay 2: light from below | 第75页 |
| 2.4 Statistical analysis | 第75页 |
| 3 Results | 第75-77页 |
| 3.1 Different lights from above affecting tree top disease | 第75-76页 |
| 3.2 Effect of lights from below on tree top disease | 第76-77页 |
| 3.3 Behavioral assay using uninfected larvae | 第77页 |
| 4 Conclusion | 第77-79页 |
| Chapter 5 Discussion and Conclusion | 第79-87页 |
| 1 General discussion | 第79-85页 |
| 1.1 Induced Hyperactive behavior in L dispar larvae | 第79-80页 |
| 1.2 Induced Tree top disease in L dispar larvae | 第80-84页 |
| 1.3 Role of light in tree top disease | 第84-85页 |
| 2 Conclusion | 第85-87页 |
| Reference | 第87-98页 |
| Acknowledgements | 第98-99页 |
| Author’s Introduction | 第99-100页 |
