| ABSTRACT | 第15-18页 |
| 摘要 | 第19-22页 |
| ABBREVIATION | 第22-24页 |
| CHAPTER 1. Background Information | 第24-33页 |
| 1.1 General Introduction | 第24-26页 |
| 1.2 Problem Statement | 第26-27页 |
| 1.3 Justification | 第27-30页 |
| 1.4 Objectives | 第30页 |
| 1.5 The Overall Outline of This Dissertation | 第30-33页 |
| CHAPTER 2. Review of Literature | 第33-59页 |
| 2.1 Introduction | 第33-34页 |
| 2.2 Current Goat Population Status | 第34-36页 |
| 2.3 Current Production Status of Goat | 第36-39页 |
| 2.4 Importance of Goat Production | 第39-41页 |
| 2.5 Gastrointestinal Nematodes (GINs) in Goat Production | 第41页 |
| 2.6 Life Cycle of Haemonchus contortus in Goat | 第41-43页 |
| 2.7 Control Methods for GINs | 第43-47页 |
| 2.7.1 Non-Genetic Control Methods for GINs | 第43-46页 |
| 2.7.2 Genetic Control of GINs | 第46-47页 |
| 2.8 Phenotypic Indicators of Resistance | 第47页 |
| 2.9 Immune Response to Haemonchus contortus | 第47-48页 |
| 2.10 Genetic Resistance to GINs Infection | 第48-51页 |
| 2.11 Heritability in FEC of Genetic Resistance | 第51页 |
| 2.12 Identification of QTL Associated with GIN Resistance | 第51-52页 |
| 2.13 Polymorphisms and Genetic Association | 第52-53页 |
| 2.14 Identified Loci Underlying Variation in Resistance to GINs | 第53-55页 |
| 2.15 Transcriptome Study | 第55-58页 |
| 2.15.1 Next-Generation Sequencing | 第55-56页 |
| 2.15.2 Gene Ontology (GO) and Pathway Analysis | 第56-57页 |
| 2.15.3 Biological Network | 第57页 |
| 2.15.4 Transcriptomic Integration and Network Analysis | 第57-58页 |
| 2.16 Summary | 第58-59页 |
| CHAPTER 3. Identification the genetic resistance genes and biosynthesis pathways to gastrointestinalnematodes infection in goat using RNA-sequencing | 第59-81页 |
| 3.1 Abstract | 第59页 |
| 3.2 Introduction | 第59-61页 |
| 3.3 Materials and Methods | 第61-66页 |
| 3.3.1 Animal Selection from Breeding Herd of Yichang White Goats (YWGs) | 第61-62页 |
| 3.3.2 Phenotypic Data Collection | 第62页 |
| 3.3.3 Nematode Challenge Trial | 第62-63页 |
| 3.3.4 Worm Counts at Necropsy | 第63页 |
| 3.3.5 Extraction of RNA and Sequencing Using RNA-Seq Technology | 第63-64页 |
| 3.3.6 Quality Control for Raw Sequencing Data | 第64页 |
| 3.3.7 Transcriptome Analysis for m RNA Data | 第64页 |
| 3.3.8 q RT-PCR for Validation of DEGs | 第64-65页 |
| 3.3.9 Statistical Analysis | 第65-66页 |
| 3.4 Results | 第66-76页 |
| 3.4.1 Egg Per Gram (EPG), Weight Gain, and Blood Parameters During BrowsingPeriod | 第66-67页 |
| 3.4.2 General Characteristics of the Goat Peripheral Blood Transcriptome | 第67-68页 |
| 3.4.3 Differentially Expressed Genes (DEGs) of Resistant and Susceptible YichangWhite Goats (YWGs) | 第68-69页 |
| 3.4.4 Gene Ontology | 第69-71页 |
| 3.4.5 Signaling Pathway Analysis of the DEGs | 第71-72页 |
| 3.4.6 Genetic Association Disease Classes of DEGs of Resistant and SusceptibleYWGs | 第72-74页 |
| 3.4.7 DEGs Confirmation by q RT-PCR | 第74-76页 |
| 3.5 Discussion | 第76-80页 |
| 3.6 Conclusions | 第80-81页 |
| CHAPTER 4. The potential role of local m Ig A, systemic s Ig E and infection related genes responsesagainst Haemonchus contortus infections in goat | 第81-99页 |
| 4.1 Abstract | 第81-82页 |
| 4.2 Introduction | 第82-83页 |
| 4.3 Materials and Methods | 第83-88页 |
| 4.3.1 Ethics Statement | 第83-84页 |
| 4.3.2 Experimental Animal Selection from Resource Populations | 第84页 |
| 4.3.3 Nematode Challenge Trial and Parasitology | 第84-86页 |
| 4.3.4. Specific Antigens for m Ig A and s Ig E | 第86页 |
| 4.3.5. The Enzyme-Linked Immunosorbent Assay (ELISA) | 第86-87页 |
| 4.3.6 Selection of Infection Related Genes, RNA Extraction from Tissues Samples, c DNA Production, and q RT-PCR | 第87-88页 |
| 4.3.7 Statistical Analysis | 第88页 |
| 4.4 Results | 第88-96页 |
| 4.4.1 Phenotypic Measurements Through Parasitology and Hematology | 第88-90页 |
| 4.4.2 The Local m Ig A Responses and Correlation Among m Ig A, Parasitological, andHematological Parameters | 第90-91页 |
| 4.4.3 Systemic s Ig E Responses and Correlation Among s Ig E, Parasitological, andHematological Parameters | 第91-93页 |
| 4.4.4 Gene Expression Analysis by q RT-PCR | 第93-96页 |
| 4.5 Discussion | 第96-98页 |
| 4.6 Conclusion | 第98-99页 |
| CHAPTER 5. The polymorphisms in ATPase sarco/ endoplasmic reticulum Ca2+ transporting-3 geneassociated with gastrointestinal nematode infection in goat | 第99-123页 |
| 5.1 Abstract | 第99-100页 |
| 5.2 Introduction | 第100-102页 |
| 5.3 Materials and Methods | 第102-107页 |
| 5.3.1 Ethics Statement | 第102页 |
| 5.3.2 Animal Selection and Generation of Phenotypic Data as GIN Infection | 第102-103页 |
| 5.3.3 Challenge Trial, Extreme Animal Selection for RNA Sequencing and GeneSelection for Polymorphisms Detection | 第103-104页 |
| 5.3.4 DNA Extraction, Polymorphisms Detection and Genotyping | 第104-105页 |
| 5.3.5 RNA Extraction, c DNA Production, and q RT-PCR | 第105-106页 |
| 5.3.6 Statistical Analysis | 第106-107页 |
| 5.4 Results | 第107-120页 |
| 5.4.1 Polymorphisms (SNPs) in m RNA Sequence of ATP2A3 Gene in Goat | 第108-109页 |
| 5.4.2 Genotypic and Allelic Frequencies of Different SNPs of ATP2A3 Gene | 第109-111页 |
| 5.4.3 Association with the Genotypes of Seven Polymorphisms in ATP2A3 Gene andGINs Infection Traits of Three Chinese Goat Populations | 第111-118页 |
| 5.4.4 Haplotype and Linkage Disequilibrium (LD) Analysis of ATP2A3 Gene inGoat Populations | 第118-119页 |
| 5.4.5 Detection of Relative Expression of ATP2A3 gene by q RT-PCR | 第119-120页 |
| 5.5 Discussion | 第120-122页 |
| 5.6 Conclusion | 第122-123页 |
| CHAPTER 6. General Conclusion and Innovation of the Study | 第123-125页 |
| 6.1 General Conclusion | 第123-124页 |
| 6.2 Innovations of the Study | 第124-125页 |
| CHAPTER 7. Recommendation and Future Direction | 第125-126页 |
| 7.1 Recommendation | 第125页 |
| 7.2 Future Direction | 第125-126页 |
| References | 第126-154页 |
| APPENDICES | 第154-187页 |
| ACKNOWLEDGEMENTS | 第187-189页 |
| Curriculum Vitae | 第189-196页 |
| About Author | 第196-197页 |
