| 摘要 | 第5-6页 |
| Abstract | 第6-7页 |
| CHAPTER 1. PROTEIN PURIFICATION | 第11-31页 |
| 1 .1 Challenges in biopharmaceutical industry | 第11-12页 |
| 1.2 Emerging bio-separation technologies | 第12-13页 |
| 1.3. Membrane chromatography: A promising bio-separation technology | 第13-21页 |
| 1.3.1 Principle of membrane chromatography | 第13-15页 |
| 1.3.2 Conventional membrane adsorbers | 第15-16页 |
| 1.3.3 Interaction modes and configurations of membrane adsorbers | 第16-18页 |
| 1.3.4 Operation modes of membrane chromatography | 第18-19页 |
| 1.3.5 Binding capacity of membrane adsorber | 第19-21页 |
| 1.4 Bioprocessing of immunoglobulins and lysozyme | 第21-28页 |
| 1.4.1 Bioprocessing of immunoglobulins | 第21-25页 |
| 1.4.2 Bioprocessing of Lysozyme | 第25-28页 |
| 1.5 Research Objectives | 第28-29页 |
| 1.6 Thesis outline | 第29-31页 |
| CHAPTER 2. MATERIALS,SYNTHESIS,CHARACTERIZATION AND SEPARATION METHODS | 第31-43页 |
| 2.1 Materials | 第31-32页 |
| 2.2 Preparation of alginate dialdehyde (ADA) | 第32-33页 |
| 2.3 Nylon membrane coating with alginate dialdehyde | 第33页 |
| 2.4 Membrane characterization | 第33-39页 |
| 2.4.1 Measuring surface zeta potential of the membrane adsorbers | 第34-35页 |
| 2.4.2 Elucidation of chemical composition of the membrane adsorbers | 第35-37页 |
| 2.4.3 Studying the morphology of the membrane adsorbers | 第37-38页 |
| 2.4.4 Measurement of contact angle of membrane adsorbers | 第38-39页 |
| 2.5 Proteins separation and purification | 第39-41页 |
| 2.6 Measurement of protein concentration | 第41-43页 |
| CHAPTER 3. DEVELOPMENT OF A VERSATILE PLATFORM FOR FACILE AND GREENFABRICATION OF MEMBRANE ADSORBERS USING ALGINATE DIALDEHYDE | 第43-63页 |
| 3.1 Introduction | 第43-44页 |
| 3.2 Experimental section | 第44-47页 |
| 3.2.1 Fabrication of membrane adsorbers based on ADA-coated nylon membranes | 第44-45页 |
| 3.2.2 Characterization and measurements | 第45-46页 |
| 3.2.3 Measurement of static binding capacity of membrane adsorbers | 第46页 |
| 3.2.4 Separation of IgG from IgG/HSA mixture | 第46-47页 |
| 3.2.5 Sample assay | 第47页 |
| 3.3 Results and discussion | 第47-61页 |
| 3.3.1 Membrane characterization | 第47-54页 |
| 3.3.2 Purification of IgG from IgG/HSA mixture | 第54-61页 |
| 3.4 Conclusion | 第61-63页 |
| CHAPTER 4. FACILE AND GREEN FABRICATION OF SYNERGETIC MEMBRANE ADSORBERSFOR ANTIBODY PURIFICATION | 第63-79页 |
| 4.1 Introduction | 第63-64页 |
| 4.2 Experimental section | 第64-67页 |
| 4.2.1 Fabrication of membrane adsorbers | 第64-65页 |
| 4.2.2 Characterization methods | 第65页 |
| 4.2.3 IgG static adsorption | 第65-66页 |
| 4.2.4 Determination of dynamic binding capacity | 第66页 |
| 4.2.5 Purification of IgG from human plasma solution | 第66-67页 |
| 4.3. Results and discussion | 第67-78页 |
| 4.3.1 Membrane characterization | 第67-70页 |
| 4.3.2 Effect of ADA coating time on the permeability of membrane adsorber | 第70页 |
| 4.3.3 Effect of pH on IgG static adsorption | 第70-71页 |
| 4.3.4 IgG adsorption isotherms for CEX and Syn. adsorbers | 第71-72页 |
| 4.3.5 Dynamic binding capacity of CEX and Syn. adsorbers | 第72-74页 |
| 4.3.6 Purification of IgG from human plasma solution | 第74-77页 |
| 4.3.7 Reusability and cost | 第77-78页 |
| 4.4. Conclusion | 第78-79页 |
| CHAPTER 5. FACILE AND GREEN FABRICATION OF CATION EXCHANGE MEMBRANEADSORBER WITH UNPRECEDENTED ADSORPTION CAPACITY FOR PROTEINPURIFICATION | 第79-101页 |
| 5.1 Introduction | 第79-81页 |
| 5.2 Experimental section | 第81-85页 |
| 5.2.1 Fabrication of ADA-nylon and sulphonyl-ADA-nylon membrane adsorbers | 第81页 |
| 5.2.2 Characterization methods | 第81-82页 |
| 5.2.3 Lysozyme static adsorption | 第82页 |
| 5.2.4 Dynamic binding capacity | 第82-83页 |
| 5.2.5 Separation of lysozyme from chicken egg white (CEW) solution | 第83-84页 |
| 5.2.6 Sample assay | 第84页 |
| 5.2.7 Reusability test | 第84-85页 |
| 5.3 Results and discussion | 第85-100页 |
| 5.3.1 Membrane characterization | 第85-89页 |
| 5.3.2 Comparison of ADA-nylon and S-ADA-nylon membrane adsorbers | 第89-90页 |
| 5.3.3 Process optimization of membrane adsorber preparation and application | 第90-95页 |
| 5.3.4 Dynamic binding capacity of S-ADA-nylon adsorbers | 第95-96页 |
| 5.3.5 Purification of lysozyme from CEW solution | 第96-98页 |
| 5.3.6 Reusability and cost | 第98-100页 |
| 5.4 Conclusion | 第100-101页 |
| CHAPTER 6. SUMMARY AND FUTURE PERSPECTIVE | 第101-105页 |
| 6.1 Summary | 第101-103页 |
| 6.1.1 Fabrication of an economic and green novel membrane platform | 第101页 |
| 6.1.2 Construction of a novel synergetic membrane adsorber for the purification of IgG from humanplasma | 第101-102页 |
| 6.1.3 Construction of a potent dual CEX membrane adsorber and its application for lysozymepurification from CEW solution | 第102-103页 |
| 6.2 Novelty | 第103-104页 |
| 6.3 Future Perspective | 第104-105页 |
| 6.3.1 Construction of different membrane adsorbers based on ADA-coated nylon platform | 第104页 |
| 6.3.2 Construction of different membrane adsorbers for IgG purification | 第104页 |
| 6.3.3 Applications of the dual CEX membrane adsorbers at industrial scale | 第104-105页 |
| REFERENCES | 第105-113页 |
| ACKNOWLEDGEMENTS | 第113-115页 |
| CURRICULUM VITAE | 第115-116页 |
