| 摘要 | 第1-10页 |
| ABSTRACT | 第10-21页 |
| PART Ⅰ.REGULATION OF B CELLS BY E2/MSC | 第21-83页 |
| CHAPTER 1 THE IMMUNE REGULATION OF B CELLS BY E2 AND MSCS | 第21-38页 |
| 1. B CELLS BACKGROUNDS | 第21-26页 |
| ·B cells activation and functions | 第21-22页 |
| ·TLRs on B cells and TLR9 | 第22-24页 |
| ·B cells and autoimmune diseases | 第24-25页 |
| ·Charactors of human B cell lines | 第25-26页 |
| 2. ESTROGEN AND ROLES ON AUTOIMMUNE REGULATION | 第26-27页 |
| 3. THE ROLE OF MSC ON IMMUNE REGULATION | 第27-31页 |
| ·The background of mesenchymal stem cell | 第27-28页 |
| ·MSC and the therapy for autoimmune diseases | 第28-29页 |
| ·The modulation of MSC on immune cells | 第29-31页 |
| 4. REFERENCE | 第31-38页 |
| CHAPTER 2 17B-ESTRADIOL ENHANCES RESPONSE OF MICE SPLEEN B CELLS ELICITED BY TLR9 AGONIST | 第38-62页 |
| 1. INTRODUCTION | 第38-39页 |
| 2. MATERIALS AND METHODS | 第39-44页 |
| ·Mice | 第39-40页 |
| ·Reagents | 第40页 |
| ·Isolation of B cells and cell culture | 第40-41页 |
| ·Viability test | 第41页 |
| ·B lymphocyte proliferation assay | 第41-42页 |
| ·Cell death assay and cell counting | 第42页 |
| ·Flow cytometry | 第42-43页 |
| ·ELISA | 第43页 |
| ·Microarray assay | 第43页 |
| ·Ovariectomy and E2 exposure | 第43-44页 |
| ·Statistical analysis | 第44页 |
| 3. RESULTS | 第44-55页 |
| ·E2 changed the morphology and density of B cells in vitro with CpG ODN stimulation | 第44-45页 |
| ·E2 improved the viability of B cells with CpG stimulation | 第45-47页 |
| ·E2 enhanced the expression of co-stimulatory molecules(CD86 and CD40)on B cells under the exposure to CpG ODN | 第47-48页 |
| ·CpG ODN induced the expression of IgM and cytokines,in which E2 enhance the IgM level in supernatant | 第48-50页 |
| ·In vivo experiments | 第50-53页 |
| ·E2 increased the expression of TLR9 and MCM6 on B cells induced by CpG | 第53-55页 |
| 4. DISCUSSION | 第55-59页 |
| 5. REFERENCE | 第59-62页 |
| CHAPTER 3 MSCS REGULATE HUMAN B CELL LINES FUNCTIONS | 第62-83页 |
| 1. INTRODUCTION | 第62-63页 |
| 2. MATERIALS AND METHODS | 第63-67页 |
| ·Culture of human MSCs | 第63-64页 |
| ·Culture of B cell lines and reagents | 第64页 |
| ·Cell cycle analysis | 第64-65页 |
| ·RT and QPCR analysis | 第65-66页 |
| ·Flow cytometry | 第66页 |
| ·Western blotting | 第66-67页 |
| ·Statistical analysis | 第67页 |
| 3. RESULTS | 第67-78页 |
| ·The influence of MSCs on the growth of B cell lines with or without CpG-DNA stimulation | 第67-69页 |
| ·Inhibition of IgM and CD86 surface expressions on B cell lines by UC-MSCs | 第69-73页 |
| ·Cytokines and chemokines | 第73页 |
| ·IL-1β primed UC-MSCs promoted chemokines expression on Daudi but not the inhibition of CD86 and IgM | 第73-76页 |
| ·ERK signal was involved in the effect of UC-MSCs on B cell lines | 第76-78页 |
| 4 DISCUSSION | 第78-81页 |
| 5 REFERENCE | 第81-83页 |
| PART Ⅱ.IL-12 IMMUNOTHERAPY OF SCC | 第83-110页 |
| CHAPTER 4 IL-12 AND CANCER IMMUNOTHERAPY | 第83-91页 |
| 1. CYTOKINE THERAPY FOR CANCER | 第83-84页 |
| 2. INSERLEUKIN-12(IL-12) | 第84-85页 |
| 3. ROLE OF IL-12 IN IMMUNOTHERAPY | 第85-86页 |
| 4. LENTIVIRAL VECTORS AND SAFETY | 第86-87页 |
| 5. SQUAMOUS CELL CARCINOMA(SCC)AND MURINE CANCER MODELS | 第87-88页 |
| 6. REFERENCES | 第88-91页 |
| CHAPTER 5 LOCALIZED IL-12 DELIVERY FOR IMMUNOTHERAPY OF SCC TUMOR~* | 第91-110页 |
| 1. INTRODUCTION | 第91-93页 |
| 2. MATERIALS AND METHODS | 第93-96页 |
| ·Lentiviral production | 第93-94页 |
| ·Tumor cell lines | 第94页 |
| ·Transduction of tumor cells and IL-12 level detection | 第94页 |
| ·Tumor cell growth kinetics | 第94-95页 |
| ·Mice | 第95页 |
| ·In vivo tumor experiments | 第95页 |
| ·T cell depletion | 第95-96页 |
| ·Blood cytokine analysis | 第96页 |
| ·Statistical analysis | 第96页 |
| 3. RESULTS | 第96-103页 |
| ·Generation of IL-12 secreting tumor cells | 第96-98页 |
| ·IL-12 producing cell lines were able to inhibit tumor development | 第98-99页 |
| ·Both CD4+ and CD8+ cells were required to eliminate IL-12 producing tumor cells | 第99页 |
| ·IL-12 secreting tumor cells led to the establishment of immune memory | 第99-101页 |
| ·A small percentage of IL-12 producing cells were able to protect mice from tumor development | 第101-102页 |
| ·Cell based IL-12 therapy did not lead to elevated systemic levels of inflammatory cytokines | 第102-103页 |
| 4. DISCUSSION | 第103-107页 |
| 5. REFERENCE | 第107-110页 |
| 博士研究生期间发表及待发表论文列表 | 第110-111页 |
| 致谢 | 第111-112页 |
