| Abstract | 第6-9页 |
| 摘要 | 第11-19页 |
| 1. Introduction | 第19-48页 |
| 1.1 Cotton Anthracnose | 第21-22页 |
| 1.2 Geographical distribution | 第22-23页 |
| 1.3 Disease description | 第23-24页 |
| 1.4 Colletotrichum gossypii | 第24-26页 |
| 1.5 Taxonomical distribution | 第26-28页 |
| 1.6 Identification techniques | 第28-30页 |
| 1.7 Molecular identification | 第30页 |
| 1.8 Polymerase chain reaction (PCR) | 第30-32页 |
| 1.9 Quantification of PCR | 第32页 |
| 1.10 Real-time PCR | 第32-34页 |
| 1.11 DNA probing technology | 第34-35页 |
| 1.12 Molecular identification of Colletotrichum species | 第35-36页 |
| 1.13 Biological control | 第36页 |
| 1.14 History | 第36-37页 |
| 1.15 Importation | 第37-38页 |
| 1.16 Augmentation | 第38页 |
| 1.17 Conservation | 第38-45页 |
| 1.18 ROS molecules | 第45-48页 |
| 2. Material and Method | 第48-80页 |
| 2.1 Sterilization of Glassware | 第48页 |
| 2.2 Disease sample collection | 第48页 |
| 2.3 Isolation of pathogen | 第48-49页 |
| 2.4 Maintaince of culture | 第49-50页 |
| 2.5 Morphological studies | 第50-51页 |
| 2.6 Molecular study | 第51-54页 |
| 2.6.1 DNA isolation and purification | 第51-52页 |
| 2.6.2 PCR Amplification | 第52-54页 |
| 2.7 Pathogenicity tests | 第54-55页 |
| 2.8 Host range of Colletotrichum gossypii | 第55-56页 |
| 2.9 Design of Colletotrichum gossypii-specific PCR primers | 第56-61页 |
| 2.9.1 PCR-based DNA amplification using SPSCG/F and SPSCG/R primers | 第56-57页 |
| 2.9.2 Specificity of primers and sensitivity of PCR | 第57-61页 |
| 2.10 DNA modeling and phylogenetic analysis | 第61-65页 |
| 2.11 Isolation of Rhizobacteria | 第65页 |
| 2.12 Isolation of Ectophyte Rhizobacteria | 第65-66页 |
| 2.13 Isolation of Endophyte Rhizobacteria | 第66页 |
| 2.14 Purification of Isolated Rhizobacteria | 第66页 |
| 2.15 Screening for antifungal activity against Colletotrichum gossypii | 第66-67页 |
| 2.16 Qualitative Screening | 第67页 |
| 2.17 Quantitative Screening | 第67-69页 |
| 2.17.1 Screening of Rhizobacteria for Indole Acetic Acid Production | 第68页 |
| 2.17.2 Screening of Rizobacteria for Phosphorous Solubilization | 第68-69页 |
| 2.18 Identification of Most Promising Rhizobacteria | 第69-71页 |
| 2.18.1 Gram Staining | 第69页 |
| 2.18.2 Spore Staining | 第69-70页 |
| 2.18.3 Catalase Test | 第70页 |
| 2.18.4 Starch Hydrolysis Test | 第70页 |
| 2.18.5 Voges Proskauer Test | 第70页 |
| 2.18.6 Cell Diameter Measurement | 第70-71页 |
| 2.18.7 Citrate Utilization Test | 第71页 |
| 2.18.8 Growth In 6.5% Sodium Chloride Lb Broth Media | 第71页 |
| 2.18.9 Growth at 55℃ | 第71页 |
| 2.19 Molecular identification | 第71-73页 |
| 2.20 Fermentation and Extraction of metabolites | 第73-74页 |
| 2.21 Antifungal assessment of extracted metabolites | 第74页 |
| 2.22 Heat stability and pH range of extracted metabolites | 第74-75页 |
| 2.23 EC50 (concentration required to obtain a 50% antifungal effect) determination | 第75页 |
| 2.24 ROS accumulation in cotton leaves | 第75-76页 |
| 2.25 Toxicity test | 第76页 |
| 2.26 Compare the fungicide and antifungal metabolites of B. licheniformis resistant against Colletotrichum gossypii | 第76-78页 |
| 2.27 Bacillus licheniformis strain OE-04 prevents disease infection on cotton seedling | 第78-80页 |
| 3. Results | 第80-117页 |
| 3.1 Strain Isolation | 第80页 |
| 3.2 Morphological Study of Colletotrichum gossypii isolates | 第80-84页 |
| 3.3 Molecular identification | 第84页 |
| 3.4 Pathogenicity evaluation | 第84-87页 |
| 3.5 Host range of Colletotrichum gossypii | 第87-88页 |
| 3.6 Sequence variation in the TUB region and the design of Colletotrichum gossypii-specific primers703.7 Specificity of primers and sensitivity of PCR | 第88-94页 |
| 3.8 DNA modeling and phylogenetic analysis | 第94-99页 |
| 3.9 Isolation of Rhizobacteria | 第99-101页 |
| 3.10 Qualitative Screening of Rhizobacteria for Antifungal Activity | 第101-103页 |
| 3.11 Quantitative Screening of Rhizobacteria for Antifungal Activity | 第103-104页 |
| 3.12 Biochemical properties of most promising antifungal rhizobacterial | 第104-105页 |
| 3.13 Molecular identification | 第105-106页 |
| 3.14 Biological activities of the OE-04 strain biosurfactant | 第106-107页 |
| 3.15 Effect of Bacillus licheniformis strain OE-04 on Fungal Hyphae | 第107-108页 |
| 3.16 Temperature, pH stability of OE-04 secreted metabolites | 第108-109页 |
| 3.17 ROS accumulation in cotton leaves | 第109-110页 |
| 3.18 Toxicity effect of antifungal substance on zebra fish | 第110-113页 |
| 3.19 Compare the fungicide and antifungal metabolites of B. licheniformis resistant against C. gossypii | 第113-116页 |
| 3.20 Bacillus licheniformis strain OE-04 prevent disease infection on cotton seedling | 第116-117页 |
| 4. Discussion | 第117-129页 |
| 5. Conclusion | 第129-133页 |
| 5.1 Anthracnose | 第129页 |
| 5.2 Morphological study | 第129-130页 |
| 5.3 Pathogenicity and host range | 第130页 |
| 5.4 Molecular detection of C. gossypii | 第130-131页 |
| 5.5 Biological control of C. gossypii | 第131-132页 |
| 5.6 Fungicide sensitivities of C. gossypii | 第132-133页 |
| Published articles | 第133页 |
| Unpublished articles | 第133-134页 |
| References | 第134-149页 |
| Acknowledgement | 第149页 |
