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中国野生毛葡萄VqJAZ7基因抗病功能分析

摘要第7-9页
ABSTRACT第9-10页
Chapter1 LITERATURE REVIEW第14-32页
    1.1 Plant Defense Mechanisms第14-15页
    1.2 Role of phytohormones in defense第15-27页
        1.2.1 Salicylic Acid第16-18页
        1.2.2 Jasmonates第18-27页
    1.3 Powdery Mildew第27-28页
    1.4 Pseudomonas syringae pv.tomato第28-30页
    1.5 Botrytis cinerea第30页
    1.6 JAZs in the Grapevine and Arabidopsis第30-32页
Chapter2 Arabidopsis VqJAZ7 Transgenic Lines Show Enhanced Resistance to Powdery Mildew第32-42页
    2.1 Objectives:第32页
    2.2 Materials and Methods第32-35页
        2.2.1 Plant and Pathogen Material and Growth Conditions第32页
        2.2.2 Bioinformatic Analyses第32页
        2.2.3.Extraction of RNA from Grapevine Leaves and Semi-Quantitative RT-PCR第32-33页
        2.2.4.Plant Transformation第33-34页
        2.2.5.Pathogen Inoculation Assays第34页
        2.2.6.Histochemical Detection第34页
        2.2.7.Quantitative real-time PCR第34-35页
        2.2.8.Statistical analysis第35页
    2.3.Results第35-40页
        2.3.1.Bioinformatic Information About VqJAZ7 Gene第35-36页
        2.3.2.Analysis of VqJAZ7 Overexpression Lines第36-37页
        2.3.3.Arabidopsis VqJAZ7 Transgenic Lines Show Enhanced Resistance to Powdery Mildew第37-40页
    2.4.Discussion第40-42页
Chapter3 Overexpression of VqJAZ7 in Arabidopsis Decreased Resistance to B.cinerea第42-50页
    3.1 Objectives:第42页
    3.2 Materials and Methods第42-44页
        3.2.1 Plant and Pathogen Material and Growth Conditions第42页
        3.2.2 Bioinformatic Analyses第42页
        3.2.3.Extraction of RNA from Grapevine Leaves and Semi-Quantitative RT-PCR第42-43页
        3.2.4.Plant Transformation第43页
        3.2.5.Pathogen Inoculation Assays第43页
        3.2.6.Histochemical Detection第43-44页
        3.2.7.Quantitative real-time PCR第44页
        3.2.8.Statistical analysis第44页
    3.3.Results第44-48页
        3.3.1.Bioinformatic Information About VqJAZ7 Gene第44-45页
        3.3.2.Analysis of VqJAZ7 Overexpression Lines第45页
        3.3.3.Overexpression of VqJAZ7 in Arabidopsis Decreased Resistance to B.cinerea第45-48页
    3.4.Discussion第48-50页
Chapter4 Overexpressing VqJAZ7 in A.thaliana Enhances Susceptibility to Pst......................................................................................................................................DC3000第50-58页
    4.1 Objectives:第50页
    4.2 Materials and Methods第50-52页
        4.2.1 Plant and Pathogen Material and Growth Conditions第50页
        4.2.2 Bioinformatic Analyses第50页
        4.2.3.Extraction of RNA from Grapevine Leaves and Semi-Quantitative RT-PCR第50页
        4.2.4.Plant Transformation第50-51页
        4.2.5.Pathogen Inoculation Assays第51页
        4.2.6.Histochemical Detection第51-52页
        4.2.7.Quantitative real-time PCR第52页
        4.2.8.Statistical analysis第52页
    4.3.Results第52-56页
        4.3.1.Bioinformatic Information About VqJAZ7 Gene第52页
        4.3.2.Analysis of VqJAZ7 Overexpression Lines第52-53页
        4.3.3.Overexpressing VqJAZ7 in A.thaliana Enhances Susceptibility to Pst DC第53-55页
        4.3.4.Over-Expressing VqJAZ7 in A.thaliana Decreases Callose Accumulation in Response to Different Treatments第55-56页
    4.4 Discussion第56-58页
Chapter5 Summary,Conclusion and Future Prospects第58-60页
    5.1 Summary第58-59页
    5.2 Conclusion and Innovation第59页
    5.3 Future Prospects第59-60页
REFERENCES第60-74页
附件第74-77页
Abbreviations第77-79页
Acknowledgement第79-80页
ABOUT AUTHOR第80页

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