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盐胁迫对水稻植株生长、籽粒发育影响及1-MCP调控作用

摘要第7-9页
ABSTRACT第9-11页
LIST OF ABBREVIATION第17-24页
CHAPTER 1 INTRODUCTION第24-39页
    1.1 INTRODUCTION第24-26页
    1.2 LITERATURE REVIEW第26-37页
        1.2.1 Salt Stress第26页
        1.2.2 Harmful effects of salt stress on plant第26-28页
        1.2.3 Effects of salt stress on rice growth and development第28-30页
        1.2.4 Effects of salt stress on rice physiological characteristics第30-32页
        1.2.5 Role of ethylene in rice growth and development第32-34页
        1.2.6 1-methylecyclopropene (1-MCP) as an ethylene inhibitor under salt stress第34-35页
        1.2.7 Effects of 1-MCP on rice spikelets development第35-37页
    1.3 QUESTIONS OF RESEARCH第37页
    1.4 OBJECTIVES AND THE SIGNIFICANCE OF THE RESEARCH第37-38页
        1.4.1 Objectives第37-38页
        1.4.2 Significance of the research第38页
    1.5 EXPERIMENTAL DESIGN第38页
    1.6 TECHINCAL ROUT第38-39页
CHAPTER 2 SODIUM CHLORIDE STRESS DURING EARLY GROWTH STAGES ALTERED PHYSIOLOGICAL AND GROWTH CHARACTERISTICS OF RICE第39-65页
    2.1 INTRODUCTION第39-40页
    2.2 MATERIALS AND METHODS第40-44页
        2.2.1 Location and growth conditions第40-41页
        2.2.2 Plant material and experimental design第41-42页
        2.2.3 Plant sampling第42页
        2.2.4 Calculations of seed emergence characteristics第42-43页
        2.2.5 Seedling height reduction第43页
        2.2.6 Relative dry weight…第43页
        2.2.7 Calculations of Growth characteristics…第43-44页
        2.2.8 Photosynthetic activities…第44页
        2.2.9 Statistical analysis第44页
    2.3 RESULTS第44-60页
        2.3.1 Effects of salt stress on seedling emergence characteristics第45-47页
        2.3.2 Growth characteristics at early seedlings stage第47-48页
        2.3.3 Seedling height reduction rate at seedling and maximum tillering stage第48-50页
        2.3.4 Physiological and growth characteristics at maximum tillering stage第50-52页
        2.3.5 Relative dry weight at seedling stage and maximum tillering stage第52-54页
        2.3.6 Tillering dynamics第54-55页
        2.3.7 Leaf Area第55-56页
        2.3.8 Growth characteristics at maximum tillering stage第56-59页
        2.3.9 Categorization of rice cultivars against sodium chloride第59-60页
    2.4 DISCUSSION第60-65页
CHAPTER 3 1-METHYLCYCLOPENE MODULATES PHYSIOLOGICAL AND AGRONOMICAL CHARACTERISTICS OF RICE TO SALT STRESS第65-87页
    3.1 INTRODUCTION第65-67页
    3.2 MATERIALS AND METHODS第67-69页
        3.2.1 Experimental design第67页
        3.2.2 Soil condition and fertilizer第67-68页
        3.2.3 1-MCP application第68页
        3.2.4 Plant sampling第68页
        3.2.5 Dry matter measurement第68页
        3.2.6 Leaf area index (LAI)第68页
        3.2.7 Physiological parameters第68-69页
        3.2.8 Statistical analysis第69页
    3.3 RESULTS第69-84页
        3.3.1 1-MCP modulates physiological characters of rice under salt stress from maximum tillering stage to full heading stage第69-74页
        3.3.2 1-MCP modulates agronomical characters第74-84页
            3.3.2.1 Leaf area index第74-75页
            3.3.2.2 1-MCP regulate total aboveground biomass at maturity第75-77页
            3.3.2.3 Effects of 1-MCP on growth characteristics at full heading stage ..第77-81页
            3.3.2.4 Effects of 1-MCP on the growth characteristics of rice at maturity第81-84页
    3.4 DISCUSSION第84-87页
CHAPTER 4 EFFECTS OF 1-METHYLCYCLOPROPENE ON RICE SUPERIOR AND INFERIOR SPIKELETS DEVELOPMENT UNDER SALT STRESS第87-104页
    4.1 INTRODUCTION第87-90页
    4.2 MATERIALS AND METHODS第90-92页
        4.2.1 Growth conditions第90页
        4.2.2 Plant materials第90页
        4.2.3 Experimental design第90页
        4.2.4 1-Methylcyclopropene application第90页
        4.2.5 Fertilizer application第90页
        4.2.6 Plant sampling第90页
        4.2.7 Spikelet grain weight and grain filling rate第90-91页
        4.2.8 Ethylene production第91页
        4.2.9 Statistical analyses第91-92页
    4.3 RESULTS第92-100页
        4.3.1 Effect of 1-MCP on ethylene production in rice spikelets under salt stress第92-93页
        4.3.2 Effect of 1-MCP on rice grain filling process under salt stress第93-97页
        4.3.3 Effects of 1-MCP on grain yield of rice and its components第97-100页
    4.4 DISCUSSION第100-104页
CHAPTER 5 1-METHYLECYCLOPROPENE REGULATE BIOCHEMICAL CHARACTERISTICS OF RICE RESPONSE TO SALT STRESS第104-120页
    5.1 INTRODUCTION第104-106页
    5.2 MATERIALS AND METHODS第106-110页
        5.2.1 Experimental design第106-107页
        5.2.2 Soil condition and fertilizer第107页
        5.2.3 1-Methylcyclopropene application第107页
        5.2.4 1 Plant sampling第107页
        5.2.5 Determination of proline, and protein第107-108页
        5.2.6 Determination of anti-oxidants第108-109页
        5.2.7 Chlorophyll contents第109-110页
        5.2.8 Statistical analysis第110页
    5.3 RESULTS第110-117页
        5.3.1 Effects of 1-MCP on antioxidants第110-113页
            5.3.1.1 Malondialdehyde第110-111页
            5.3.1.2 Superoxide dismutase activity第111-112页
            5.3.1.3 Hydrogen per Oxide第112-113页
        5.3.2 Proline contents第113-114页
        5.3.3 Soluble protein第114-115页
        5.3.4 Chlorophyll a, b, carotenoids, and total chlorophyll第115-117页
    5.4 DISCUSSION第117-120页
CHAPTER 6 EFFECTS OF SALT STRESS ON RICE PROTEOMICS AND BIOCHEMICAL FEATURES第120-153页
    6.1 INTRODUCTION第120-122页
    6.2 MATERIALS AND METHODS第122-129页
        6.2.1 Growth conditions, Plants material, and experimental design第122页
        6.2.2 Soil and plant sampling第122-123页
        6.2.3 Leaf proteomics analysis第123-126页
            6.2.3.1 Protein Extraction第123页
            6.2.3.2 QC of Protein Extraction第123-124页
            6.2.3.3 Protein Digestion第124页
            6.2.3.4 Peptide Labeling第124页
            6.2.3.5 Peptide Fractionation第124-125页
            6.2.3.6 HPLC第125页
            6.2.3.7 Mass Spectrometer Detection第125-126页
        6.2.4 Cell membrane injury第126-127页
        6.2.5 Rice root activity第127页
        6.2.6 Na~+ concentration in soil and plant第127-128页
        6.2.8 Cl~- concentration in soil and plant第128-129页
        6.2.10 Statistical analysis第129页
    6.3 RESULTS第129-149页
        6.3.1 Na~+ and Cl~- in soil第129-130页
            6.3.1.1 Na~+ concentration in soil第129页
            6.3.1.2 Cl~- concentration in soil第129-130页
        6.3.2 Na~+ and Cl~- in rice plant第130-132页
            6.3.2.1 Na~+ concentration in rice roots and leaves第130页
            6.3.2.2 Cl~- concentration in rice roots and leaves第130-132页
        6.3.3 Effects of Na Cl on rice flag leave’s Cell membrane injury第132页
        6.3.4 Effects of Na Cl rice root activity第132-133页
        6.3.5 Effects of Na Cl on leaf proteomics in rice at maximum tillering stage第133-149页
            6.3.5.1 Salt stress-response upregulated proteins第135-141页
            6.3.5.2 Unknown function upregulated proteins under salt stress第141-149页
    6.4 DISCUSSION第149-153页
CHAPTER 7 MAJOR FINDINGS AND FUTURE PERSPECTIVES第153-156页
    7.1 NOVEL FINDINGS第153页
    7.2 MAJOR FINDINGS第153-154页
    7.3 FUTURE PERSPECTIVES第154-156页
REFERENCES第156-179页
LIST OF PUBLICATIONS第179-181页
ACKNOWLEDGEMENTS第181-183页
AUTHOR RESUME第183页

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