| 摘要 | 第9-12页 |
| Abstract | 第12-15页 |
| List of abbreviations | 第16-18页 |
| CHAPTER-1 General introduction | 第18-29页 |
| 1.1 Overview of visceral pain | 第18-19页 |
| 1.2 Role of cytokines in pain | 第19-21页 |
| 1.3 Mast cells and pain | 第21页 |
| 1.4 MAPKs and Pain | 第21-23页 |
| 1.5 p38MAPK and pain | 第23页 |
| 1.6 Electro-acupuncture | 第23-27页 |
| 1.7 Objectives of the proposed research | 第27-29页 |
| CHAPTER 2 Visceral pain triggered by traction on the ileocecal ligament with ileitis | 第29-47页 |
| 2.1 Materials and methods | 第30-36页 |
| 2.1.1 Experimental animal and grouping | 第30页 |
| 2.1.2 Induction of experimental ileitis | 第30-32页 |
| 2.1.3 The observation of pain behavioral responses | 第32-33页 |
| 2.1.4 Sample collection | 第33-34页 |
| 2.1.5 Macroscopic and microscopic observations of ileal tissue | 第34-35页 |
| 2.1.6 Measurement of cytokines and myeloperoxidase | 第35页 |
| 2.1.7 Statistical analysis | 第35-36页 |
| 2.2 Results | 第36-44页 |
| 2.2.1 Behavioral responses to traction on ileocecal ligament | 第36-37页 |
| 2.2.2 Percentage body weight changes in treated goats | 第37-38页 |
| 2.2.3 Ileal macroscopic features of treated goats | 第38-40页 |
| 2.2.4 Ileal microscopic changes in experimental goats | 第40-42页 |
| 2.2.5 Changes in ileal cytokines and myeloperoxidase concentrations | 第42-44页 |
| 2.3 Discussion | 第44-47页 |
| CHAPTER 3 Cumulative effect of electroacupuncture on p38MAPK and c-Fosexpression in the spinal dorsal horn of goats with visceral hypersensitivity | 第47-69页 |
| Introduction | 第47-49页 |
| 3.1 Materials and methods | 第49-56页 |
| 3.1.1 Experimental Animal and Grouping | 第49页 |
| 3.1.2 Animal model of visceral hypersensitivity | 第49-50页 |
| 3.1.3 Ileal sample collection | 第50-51页 |
| 3.1.4 Microscopic observations | 第51-52页 |
| 3.1.5 Electroacupuncture stimulation | 第52页 |
| 3.1.6 Recording of visceromotor response to colorectal distension | 第52-53页 |
| 3.1.7 Immunohistochemistry | 第53-54页 |
| 3.1.8 Western blotting | 第54-55页 |
| 3.1.9 RT-PCR | 第55页 |
| 3.1.10 Statistical Analysis | 第55-56页 |
| 3.2 Results | 第56-66页 |
| 3.2.1 Microscopic scoring of ileitis in experimental goats | 第56-58页 |
| 3.2.2 Cumulative effect of electroacupuncture on microscopic changes in goats | 第58-59页 |
| 3.2.3 Effect of electroacupuncture on visceromotor response in goats | 第59-61页 |
| 3.2.4 Cumulative effect of electroacupuncture on spinal p38MAPK- IR cells and c-Fos-IR cells in goats with TNBS-induced ileitis | 第61-63页 |
| 3.2.5 Cumulative EA treatments suppress spinal p38MAPK and c-Fos proteinexpression in TNBS-induced ileitis goats | 第63-66页 |
| 3.3 Discussion | 第66-69页 |
| CHAPTER 4 | 第69-70页 |
| 4.1 Conclusions | 第69-70页 |
| 4.1.1 experiment-1 | 第69页 |
| 4.1.2 experiment-2 | 第69-70页 |
| References | 第70-83页 |
| ACKNOWLEDGEMENTS | 第83-86页 |
| Dedication | 第86-87页 |
| 作者简介 | 第87-91页 |
| List of Original Publications | 第91页 |
| Publication under process | 第91-92页 |
| Appendix | 第92-99页 |
| Microscopic pictures | 第92-94页 |
| Mast cells pictures | 第94-99页 |
