| 摘要 | 第8-10页 |
| Abstract | 第10-11页 |
| Abbreviations | 第12-13页 |
| CHAPTER Ⅰ Introduction | 第13-35页 |
| 1.1 Effects of drought and salt stress on cotton | 第14-16页 |
| 1.2 Morpho-physiological strategies of cotton to enhance drought and salt tolerance. | 第16-20页 |
| 1.2.1 Stomata regulation | 第16-17页 |
| 1.2.2 Root development | 第17-18页 |
| 1.2.3 Photosynthesis | 第18-19页 |
| 1.2.4 Osmotic adjustment | 第19-20页 |
| 1.3 Biochemical strategies of cotton to enhance drought and salt tolerance | 第20-28页 |
| 1.3.1 Abscisic acid | 第20-22页 |
| 1.3.2 Jasmonic acid | 第22-24页 |
| 1.3.3 Reactive oxygen species | 第24-28页 |
| 1.4 Scientific enhancement of drought and salt tolerance in cotton | 第28-30页 |
| 1.5 Stress-related transcription factors | 第30-34页 |
| 1.5.1 WRKY transcription factors | 第32-33页 |
| 1.5.2 MYB transcription factors | 第33-34页 |
| 1.6 Aims of the study | 第34-35页 |
| CHAPTER Ⅱ Materials and methods | 第35-48页 |
| 2.1 Sequence analysis of GhMYB108-like and GhWRKY6-like | 第35-36页 |
| 2.2 Subcellular localization of the GhMYB108-like and GhWRKY6-like proteins.. | 第36-37页 |
| 2.3 Vector construction and transformation in cotton | 第37页 |
| 2.4 Plant material, growth conditions and treatments | 第37-39页 |
| 2.5 DNA extraction and southern blotting | 第39页 |
| 2.6 RNA extraction, RT-PCR and q RT-PCR | 第39-40页 |
| 2.7 Tissue culturing and generation of homozygous transgenic lines | 第40-41页 |
| 2.8 Transformation in Arabidopsis | 第41页 |
| 2.9 Germination rate and root length of overexpressing GhWRKY6-like Arabidopsisunder salt, mannitol and ABA treatments | 第41-42页 |
| 2.10 Root length of overexpressing GhMYB108-like cotton seedlings under salt andmannitol treatments | 第42页 |
| 2.11 Determination of MDA, H_2O_2, proline content | 第42-43页 |
| 2.12 Detection of SOD and POD activity | 第43-44页 |
| 2.13 Virus-induced gene silencing (VIGS) of the GhWRKY6-like and GhMYB108-like | 第44-45页 |
| 2.14 Effects of GhWRKY6-like and GhMYB108-like on the drought and salt stress-related candidate genes | 第45-46页 |
| 2.15 Yeast two hybrid (Y2H) assay | 第46页 |
| 2.16 Statistical analysis | 第46-48页 |
| CHAPTER Ⅲ Results | 第48-81页 |
| 3.1 GhWRKY6-like gene | 第48-64页 |
| 3.1.1 Characteristics of the GhWRKY6-like gene | 第48-49页 |
| 3.1.2 Interested cis-elements found in the promotor region of GhWRKY6-like | 第49-50页 |
| 3.1.3 Subcellular localization and expression of GhWRKY6-like induced by variousabiotic stresses | 第50-51页 |
| 3.1.4 Overexpression of GhWRKY6-like enhanced germination rate under salt stress | 第51-53页 |
| 3.1.5 Root elongation of overexpressing GhWRKY6-like plants under salt stress | 第53-54页 |
| 3.1.6 Enhanced salt tolerance in overexpressing GhWRKY6-like plants and oxidativestress pathway | 第54-56页 |
| 3.1.7 Higher germination rate of overexpressing GhWRKY6-like plants undermannitol treatment | 第56页 |
| 3.1.8 Root elongation of overexpressing GhWRKY6-like plants under mannitoltreatment | 第56-57页 |
| 3.1.9 Decreased sensitivity to ABA in overexpressing GhWRKY6-like plants | 第57-58页 |
| 3.1.10 Osmotic stress and ABA Signalling pathway | 第58-60页 |
| 3.1.11 Y2H showed GhWRKY6-like interacts with ubiquitin domain containingprotein (DSKa2-like) and thaumatin like protein (TLP) | 第60-61页 |
| 3.1.12 Enhanced drought and salt sensitivity in GhWRKY6-like VIGS plants | 第61-64页 |
| 3.2 GhMYB108-like | 第64-81页 |
| 3.2.1 GhMYB108-like characteristics | 第64-65页 |
| 3.2.2 Interested cis-elements in the upstream region of GhMYB108-like | 第65-66页 |
| 3.2.3 Subcellular localization and expression of GhMYB108-like | 第66-67页 |
| 3.2.4 Overexpression of GhMYB108-like in cotton and Arabidopsis | 第67-68页 |
| 3.2.5 Confirmation of GhMYB108-like transgenes and generation of homozygouslines | 第68-69页 |
| 3.2.6 Overexpression of GhMYB108-like supressed root elongation under salt stress | 第69-71页 |
| 3.2.7 Overexpressing GhMYB108-like enhanced salt sensitivity in Arabidopsis andcotton | 第71-72页 |
| 3.2.8 Level of ROS and its scavenging in overexpressing GhMYB108-like plantsunder salt stress | 第72-74页 |
| 3.2.9 Overexpressing GhMYB108-like supressed root longevity under mannitoltreatment | 第74-76页 |
| 3.2.10 Overexpressing GhMYB108-like induced drought sensitivity in cotton andArabidopsis | 第76-77页 |
| 3.2.11 Drought stress enhanced ROS and decreased the enzymatic activities of itsscavenging in overexpressing GhMYB108-like plants | 第77-79页 |
| 3.2.12 GhMYB108-like autonomously activated | 第79页 |
| 3.2.13 Enhanced drought and salt tolerance in GhMYB108-like VIGS plants | 第79-81页 |
| CHAPTER Ⅳ Discussion | 第81-90页 |
| 4.1 GhMYB108-like and GhWRKY6-like response to drought and salt stress | 第81-82页 |
| 4.2 Cis-elements of GhMYB108-like and GhWRKY6-like involved in drought and saltstress responses | 第82-83页 |
| 4.3 Overexpressing GhWRKY6-like improved germination under salt and osmoticstresses | 第83-84页 |
| 4.4 Root development regulate drought and salt tolerance | 第84-85页 |
| 4.5 Overexpressing GhMYB108-like and GhWRKY6-like modulate chlorophyllcontent | 第85页 |
| 4.6 GhMYB108-like and GhWRKY6-like involved in response to salt stress | 第85-87页 |
| 4.7 GhMYB108-like and GhWRKY6-like regulate drought tolerance | 第87-88页 |
| 4.8 GhMYB108-like and GhWRKY6-like regulated the production of ROS understresses | 第88-90页 |
| Conclusions and future recommendations | 第90-92页 |
| References | 第92-113页 |
| Appendices | 第113-122页 |
| Appendix 1 | 第113-114页 |
| Appendix 2 | 第114-115页 |
| Appendix 3 | 第115-116页 |
| Appendix 4 | 第116-117页 |
| Appendix 5 | 第117-118页 |
| Appendix 6 | 第118-120页 |
| Appendix 7 | 第120-121页 |
| Appendix 8 | 第121-122页 |
| Acknowledgements | 第122-123页 |
| List of publications | 第123-124页 |
