| Abstract | 第4-5页 |
| 摘要 | 第5-9页 |
| 1. Introduction | 第9-15页 |
| 2. Materials and methods | 第15-30页 |
| 2.1 Materials | 第15-21页 |
| 2.1.1 Strain of bacteria, enzymes, reagents | 第15-18页 |
| 2.1.2 Equipments | 第18-21页 |
| 2.2 Methods | 第21-30页 |
| 2.2.1 Cells resuscitating, stocking and plasmid sequencing | 第21页 |
| 2.2.2 Preparing the human recombinant hemoglobin | 第21-26页 |
| 2.2.3 Analyzing the losing rate of the recombinant hemoglobin during the process | 第26-28页 |
| 2.2.4 Proportional analysis in normal and abnormal heme inserted recombinant hemoglobin expressed from Escherichia coli | 第28-30页 |
| 3. Results | 第30-43页 |
| 3.1 The sequencing result of cells | 第30-31页 |
| 3.2 The preparation of human recombinant hemoglobin | 第31-33页 |
| 3.2.1 The result of Q1 column | 第31-32页 |
| 3.2.2 The result of Q2 column | 第32-33页 |
| 3.3 Analyzing the losing rate of the recombinant hemoglobin during the procedures of lysate cells to chromatographic purification steps | 第33-39页 |
| 3.3.1 The table for samples prepare in spectro photometry | 第33-34页 |
| 3.3.2 Spectro photometry in analyzing the recovery rate of the recombinant hemoglobin during the process | 第34-36页 |
| 3.3.3 The table for sample perpare in SDS-PAGE | 第36页 |
| 3.3.4 SDS-PAGE in analyzing the content in each step and the losing rate of the recombinant hemoglobin during the process | 第36-37页 |
| 3.3.5 Analysis the gray scale of the bands on the SDS-PAGE result | 第37-38页 |
| 3.3.6 Comparison of two analysis methods in recovery rate | 第38-39页 |
| 3.4 The results of the proportional analysis in normal and abnormal heme inserted recombinant hemoglobin expressed from Escherichia coli | 第39-43页 |
| 3.4.1 the proportional analysis in normal and abnormal heme inserted recombinant hemoglobin expressed from Escherichia coli by using non-denaturing gel electrophoresis | 第39-41页 |
| 3.4.2 the proportional analysis in normal and abnormal heme inserted recombinant hemoglobin expressed from Escherichia coli by using capillary zone electrophoresis (CZE) | 第41-43页 |
| 4. Discussion | 第43-49页 |
| 4.1 Evaluation on methods can worth in normal and abnormal heme inserted recombinant hemoglobin proportional analysis | 第43-46页 |
| 4.2 Discuss the result of non-denaturing gel electrophoresis and capillary electrophoresis in this paper | 第46-48页 |
| 4.2.1 The result of non-denaturing gel electrophoresis | 第46页 |
| 4.2.2 The result of capillary zone electrophoresis | 第46-48页 |
| 4.3 The value of the result in capillary zone electrophoresis | 第48-49页 |
| 5. Conclusion | 第49-50页 |
| Appendix | 第50-57页 |
| Reference | 第57-60页 |
| Acknowledgement | 第60页 |
