| 摘要 | 第9-10页 |
| Abstract | 第10-11页 |
| 1 Introduction | 第12-29页 |
| 1.1 Purpose and Significance of Research | 第12页 |
| 1.2 Research Progress on Domestic and International level | 第12-29页 |
| 1.2.1 Chilling Injury | 第12-13页 |
| 1.2.2 Responses of Crops to Cold stress | 第13-14页 |
| 1.2.3 Effect of Cold Stress on the Growth and Development of Maize | 第14-17页 |
| 1.2.4 Effect of Cold Stress on the Physiological Mechanism of Maize | 第17-22页 |
| 1.2.5 Cold Stress Induced Genes in Maize | 第22页 |
| 1.2.6 Genetic Research on Maize under Cold Stress | 第22-23页 |
| 1.2.7 Chilling Injury Prediction Technology Research Progress | 第23-25页 |
| 1.2.8 Research Progress of Chemical Control Technology to Improve Cold Resistance | 第25-29页 |
| 2 Materials and Methods | 第29-36页 |
| 2.1 Experiment Material | 第29页 |
| 2.2 Experimental Design and Methods | 第29-35页 |
| 2.2.1 Cold Tolerance Identification at Germination Stage | 第29-30页 |
| 2.2.2 Cold Tolerance Identification | 第30-31页 |
| 2.2.3 Determination of Different Physiological and Biochemical Indicators of Resistant Varieties | 第31-32页 |
| 2.2.4 SA Concentration Screening Test | 第32-33页 |
| 2.2.5 Regulation Experiment of SA under Cold Stress on Maize Seedlings | 第33-35页 |
| 2.3 Data Analysis and Software | 第35-36页 |
| 3 Results and Analysis | 第36-84页 |
| 3.1 Cold Resistance Identification at Germination Stage | 第36-38页 |
| 3.1.1 Cold Stress Treatment Test at Germination Stage | 第36页 |
| 3.1.2 The Germination Rate and Relative Germination Rate of all Varieties under Cold Stress | 第36-37页 |
| 3.1.3 Cluster Analysis of the Relative Germination Rate of all Varieties at Cold Stress | 第37-38页 |
| 3.2 Cold Resistance Identification at Seedling Stage | 第38-43页 |
| 3.2.1 Leaf Relative Conductivity | 第38-39页 |
| 3.2.2 Leaf Relative MDA Content | 第39-40页 |
| 3.2.3 Protective Enzyme Activity of Leaves | 第40-43页 |
| 3.3 Effect of Chilling Stress on Photosynthetic Parameters of Maize Seedling | 第43-44页 |
| 3.4 Effects of Cold Stress on Chlorophyll Fluorescence Parameters in Leaves of Maize Seedlings | 第44-46页 |
| 3.4.1 Fo changes in Leaves of Maize Seedling | 第44-45页 |
| 3.4.2 Fv/Fo changes in Leaves of Maize Seedlings | 第45页 |
| 3.4.3 Fv/Fm changes in Leaves of Maize Seedlings | 第45-46页 |
| 3.5 Effect of Cold Stress on Protective Enzyme Activities in Leaves of Maize Seedlings | 第46-48页 |
| 3.5.1 Superoxide Dismutase (SOD) Activity Changes in Leaves | 第46-47页 |
| 3.5.2 Catalase (POD) Activity Changes in Leaves | 第47-48页 |
| 3.6 Effect of Cold Stress on MDA Content in Leaves of Maize Seedling | 第48-49页 |
| 3.7 Effect of Cold Stress on the Membrane Permeability of Maize Seedling | 第49-50页 |
| 3.8 Photosynthetic Parameters Changes after Restoration at Normal Temperature in Maize Seedlings after Cold Stress | 第50-52页 |
| 3.9 Chlorophyll Fluorescence Parameters Changes after Restoration at Normal Temperature in Maize Seedling after Cold Stress | 第52-61页 |
| 3.9.1 Fo changes in Leaves of Maize Seedlings | 第52-55页 |
| 3.9.2 Fv/Fo changes in Leaves of Maize Seedlings | 第55-58页 |
| 3.9.3 Fv/Fm Changes in Leaves of Maize Seedlings | 第58-61页 |
| 3.10 Protective Enzyme Activity Changes after Restoration at Normal Temperature in Maize Seedling after Cold Stress | 第61-67页 |
| 3.10.1 SOD Activity Changes in Leaves of Maize Seedlings | 第61-64页 |
| 3.10.2 POD activity changes in Leaves of Maize Seedlings | 第64-67页 |
| 3.11 Cell Membrane Permeability Changes after Restoration at Normal Temperature in the Maize Seedling after Cold Stress | 第67-68页 |
| 3.12 Leaf MDA Content Changes after Restoration at Normal Temperature in the Maize Seedlings after Cold Stress | 第68-70页 |
| 3.13 Changes in Leaf Growth after Restoration at Normal Temperature in Maize seedling after Cold Stress | 第70-71页 |
| 3.14 Screening of SA Concentration under Cold Stress | 第71-84页 |
| 3.14.1 Effects of Different Concentrations of SA on Morphological Parameters and Dry Fresh Weight of Maize Seedlings under Cold Stress | 第71-75页 |
| 3.14.2 Effects of Different Concentrations of SA on Cell Membrane Permeability of Maize Seedlings under Cold Stress | 第75-76页 |
| 3.14.3 Effect of Different Concentrations of SA on Relative Water Content of Maize Seedlings under Cold Stress | 第76-77页 |
| 3.14.4 Effects of Different Concentrations of SA on Photosynthesis of Maize Seedlings under Cold Stress | 第77-84页 |
| 4 Discussion | 第84-96页 |
| 4.1 Cold Resistance Identification Methods in Maize Inbred Lines | 第84-85页 |
| 4.2 Morphology and Germination rate in Maize Seeds | 第85页 |
| 4.3 Cold Resistance and Material Handling Methods | 第85-86页 |
| 4.4 Chlorophyll Fluorescence Parameters and Maize Cold Resistance | 第86-87页 |
| 4.5 Cell Membrane Permeability and Maize Cold Resistance | 第87-88页 |
| 4.6 Malondialdehyde Content and Maize Cold Resistance | 第88-90页 |
| 4.7 Cell Protective Enzyme System and Maize Cold Resistance | 第90-91页 |
| 4.8 Effect of SA treatment on Growth of Maize Seedling | 第91-92页 |
| 4.9 SA Induced Cold Tolerance in Maize Seedlings | 第92-95页 |
| 4.9.2 SA Induces Chilling Resistance and Photosynthetic System in Maize Seedlings | 第94-95页 |
| 4.10 deficiencies in the experiment | 第95-96页 |
| 5 Conclusions | 第96-98页 |
| Acknowledgements | 第98-100页 |
| References | 第100-112页 |
| Papers published in the period of Ph.D. education | 第112页 |
