| 摘要 | 第7-9页 |
| ABSTRACT | 第9-10页 |
| LIST OF ABBREVIATIONS | 第11-13页 |
| CHAPTER 1 INTRODUCTION AND REVIEW OF LITERATURE | 第13-26页 |
| 1. CATFISH CULTURE | 第13-14页 |
| 2. DISEASES OF CATFISH | 第14-15页 |
| 3. OVERVIEW OF FISH IMMUNITY | 第15-18页 |
| 4. IMMUNE-RELEVANT PROTEIN IN FISH | 第18-25页 |
| 4.1. RAG2 | 第18-19页 |
| 4.2. CYCLOPHILIN A | 第19-21页 |
| 4.3. MECHANISM OF CYPA CHEMOTAXIS AND LEUKOCYTE ACTIVATION | 第21-22页 |
| 4.4. CYPA SERVES AS A CO-FACTOR IN LEUKOCYTE CHEMOTAXIS | 第22-23页 |
| 4.5. CYPA INDUCED LEUKOCYTE CHEMOTAXIS | 第23-24页 |
| 4.6. INHIBITION OF THE CHEMOTACTIC ACTIVITY OF CYPA | 第24-25页 |
| 5. OBJECTIVES | 第25-26页 |
| CHAPTER 2 CHANNEL CATFISH VIRUS INFECTION IN CHANNEL CATFISHOVARY CELLS | 第26-51页 |
| 1. INTRODUCTION | 第26-27页 |
| 2. MATERIALS AND METHODS | 第27-32页 |
| 2.1. CELL CULTURE, CCV INFECTION AND SAMPLE COLLECTION | 第27-28页 |
| 2.2. EXTRACTION OF TOTAL RNA, PURIFICATION OF MRNA AND SEQUENCING OF CDNA | 第28页 |
| 2.3. CLEANING OF INITIAL (RAW) DATA,CONSTRUCTION OF THE DE-NOVO ASSEMBLY ANDANNOTATION OF THE UNIGENE | 第28-29页 |
| 2.4. EVALUATION OF THE EXPRESSION LEVEL OF GENES | 第29-30页 |
| 2.5. VERIFICATION OF THE EXPRESSION LEVEL OF GENES BY QRT-PCR | 第30-31页 |
| 2.6. APOPTOSIS EVALUATION IN CCO CELLS USING STAINING | 第31页 |
| 2.7. CASPASES ASSAY | 第31-32页 |
| 2.8. Statistics | 第32页 |
| 3. RESULTS | 第32-46页 |
| 3.1. CONSTRUCTION AND ANALYSIS OF THE TRANSCRIPTOMIC SEQUENCES ASSEMBLY | 第32-36页 |
| 3.2. EVALUATION OF THE FUNCTIONALLY ANNOTATED DEGS | 第36-41页 |
| 3.3. EVALUATION OF THE UP AND DOWN REGULATED UNIGENES | 第41页 |
| 3.4. CONFIRMATION OF THE TRANSCRIPTOMIC GENES VIA QRT-PCR | 第41-44页 |
| 3.5. THE APOPTOSIS INDUCED BY CCV INFECTION IN CCO CELLS | 第44-45页 |
| 3.6. CCV INFECTION INDUCED APOPTOSIS VIA EXTRINSIC PATHWAY IN CCO CELLS | 第45-46页 |
| 4. DISCUSSION | 第46-51页 |
| CHAPTER 3 ANALYSIS OF RAG2 IN CHINESE YELLOW CATFISH | 第51-70页 |
| 1. INTRODUCTION | 第51-52页 |
| 2. MATERIAL AND METHODS | 第52-57页 |
| 2.1. FISH | 第52页 |
| 2.2. ISOLATION (CLONING AND SEQUENCING) OF RAG2 | 第52-53页 |
| 2.3. SEQUENCE CHARACTERIZATION AND PHYLOGENETIC ANALYSIS | 第53-54页 |
| 2.4. PRODUCTION OF YC-RAG2 POLYCLONAL ANTIBODY | 第54-55页 |
| 2.5. ANALYSIS OF YELLOW CATFISH RAG2 TRANSCRIPT BY QRT-PCR | 第55页 |
| 2.6. EXAMINATION OF YELLOW CATFISH RAG2 PROTEINS BY WESTERN BLOT | 第55-56页 |
| 2.7. ANALYSIS OF THE TRANSCRIPT EXPRESSION LEVEL OF RAG2,IL-1B AND TNF-A INFISH TISSUES AFTER E. ICTALURI INFECTION | 第56页 |
| 2.8. EXAMINATION OF YC-RAG2 PROTEIN DISTRIBUTION AFTER E.ICTALURI INFECTION | 第56页 |
| 2.9. Statistics | 第56-57页 |
| 3. RESULTS | 第57-68页 |
| 3.1. CLONING, CLASSIFICATION AND PHYLOGENETIC EVALUATION OF RAG2 SEQUENCE | 第57-60页 |
| 3.2. ANTIBODY SPECIFICITY AND EXPRESSION LEVEL OF YC-RAG2 IN VARIOUS TISSUES | 第60-65页 |
| 3.3. THE TRANSCRIPT EXPRESSION OF YC-RAG2 IN E. ICTALURI CHALLENGED FISH | 第65-66页 |
| 3.4. THE LEVEL OF EXPRESSIONS OF IL-1 B AND TNF-A IN LYMPHOID TISSUE OF YELLOWCATFISH AFTER BACTERIAL INFECTION | 第66-68页 |
| 4. DISCUSSION | 第68-70页 |
| CHAPTER 4 FUNCTIONAL ANALYSIS OF CYCLOPHILIN A | 第70-85页 |
| 1. INTRODUCTION | 第70-72页 |
| 2. MATERIALS AND METHODS | 第72-78页 |
| 2.1. ANIMALS AND SAMPLE COLLECTION | 第72-73页 |
| 2.2. MASS SPECTROMETRIC ANALYSIS | 第73页 |
| 2.3. WESTERN BLOT ANALYSIS | 第73-74页 |
| 2.4. SAMPLE PREPARATION, PLASMIDS CONSTRUCTION AND PROTEIN EXPRESSION | 第74-77页 |
| 2.5. LEUKOCYTES SEPARATION | 第77页 |
| 2.6. CHEMOTAXIS ASSAY OF SKIN MUCUS | 第77-78页 |
| 2.7. CHEMOTAXIS ASSAY OF CYPA RECOMBINANT PROTEIN | 第78页 |
| 2.8. STATISTICS | 第78页 |
| 3. RESULTS | 第78-83页 |
| 3.1. MASS SPECTROMETRY AND WESTERN BLOT ANALYSIS | 第78-80页 |
| 3.2. CHEMOTAXIS ASSAY OF SKIN MUCUS | 第80-81页 |
| 3.3. CHEMOTACTIC ACTIVITY OF MUTANT CYPA | 第81-83页 |
| 4. DISCUSSION | 第83-85页 |
| CONCLUSIONS | 第85-86页 |
| REFERENCES | 第86-109页 |
| LIST OF PUBLICATIONS | 第109页 |
| Submitted papers | 第109-110页 |
| ACKNOWLEDGEMENTS | 第110页 |
