| 摘要 | 第1-13页 |
| ABSTRACT | 第13-16页 |
| Part 1:Literature Review | 第16-28页 |
| Chapter 1:GENERAL SITUATION ON EMBRYO PRODUCTION IN FARM ANIMALS | 第16-28页 |
| ·SUCCESS OF EMBRYO PRODUCTION BY ICSI METHOD IN PIG AND OTHER FARM ANIMALS | 第17-20页 |
| ·ICSI PROCESS AND ELECTRIC ACTIVATION OF OOCYTES | 第20-21页 |
| ·THE STATUS OF IN VITRO FERTILIZATION TECHNIQUE IN EMBRYO PRODUCTION | 第21-22页 |
| ·THE PROSPECTS OF SOMATIC CELL NUCLEAR TRANSFER | 第22-28页 |
| ·SELECTION OF DONOR CELLS | 第22-23页 |
| ·ENUCLEATION OF RECIPIENT CELLS AND NUCLEAR TRANSFER | 第23-24页 |
| ·ACTIVATION | 第24页 |
| ·EMBRYO CULTURE | 第24-25页 |
| ·REMODELLING AND REPROGRAMMING | 第25-28页 |
| Part 2:EXPERIMENTAL STUDY | 第28-78页 |
| Chapter 2:MALAWI SITUATION ON PIG AND OTHER FARM ANIMAL GENETIC RESOURCESCONSERVATION | 第28-56页 |
| ·INTRODUCTION | 第28-33页 |
| ·PREVIOUS AND ONGOING EFFORTS BY OTHER SCIENTISTS IN MALAWI | 第30-31页 |
| ·CROSSBREEDING PROGRAMME | 第31-32页 |
| ·PIGS | 第32页 |
| ·CATTLE | 第32-33页 |
| ·SMALL RUMINANTS | 第33页 |
| ·CHICKENS | 第33页 |
| ·SELECTION | 第33-36页 |
| ·CATTLE | 第33-34页 |
| ·SMALL RUMINANTS | 第34-36页 |
| ·POULTRY | 第36页 |
| ·PIGS | 第36页 |
| ·CURRENT NATIONAL STRATEGIES FOR CONSERVATION,IMPRVEMENT AND UTILIZATION OF FARM ANIMAL GENETIC RESOURCES(AnGRs) | 第36-41页 |
| ·BREED SURVEY AND FIELD GUIDES | 第37页 |
| ·COMMUNITY BASED LIVESTOCK ACTIVITIES | 第37页 |
| ·PROPOSALS | 第37-38页 |
| ·NATIONAL BIODIVERSITY STRATEGIES ACTION PLAN | 第38页 |
| ·CONSTRAITS IN GENETIC IMPROVEMENT PROGRAMMES | 第38-39页 |
| ·MALAWI LOCAL PIG BREED | 第39-40页 |
| ·PIG NUMBERS AND OWNERSHIP PATTERN | 第40-41页 |
| ·THE VALUE OF LOCAL PIGS AMONG SMALLHOLDER FARMERS IN MALAWI | 第41-47页 |
| ·POVERTY ALLEVIATION PERSPECTIVE | 第41-42页 |
| ·AS SOURCE OF FOOD | 第42-43页 |
| ·TOLERANCE TO DISEASES AND PARASITES | 第43-44页 |
| ·DISASTER PREPAREDNESS | 第44页 |
| ·RESEARCH NEEDS | 第44-45页 |
| ·STRATEGIES TO CONSERVE LOCAL PIGS | 第45页 |
| ·THREATS TO INDIGENOUS PIGS | 第45-46页 |
| ·STAKEHOLDERS IN CONSERVATION OF PIG BREEDS | 第46-47页 |
| ·METHODS OF PIG BREEDS CONSERVATION | 第47-48页 |
| ·CRITERIA FOR CONSERVATION | 第48-49页 |
| ·POLICY | 第48-49页 |
| ·CONCLUSION | 第49页 |
| ·JUSTIFICATION/NEED FOR CONSERVING MALAWI LOCAL PIG GENETIC RESOURCES | 第49-50页 |
| ·GOVERNMENT POLICY ON PIG PRODUCTION | 第49页 |
| ·GOVERNMENT EFFORTS TO PROMOTE PIG FARMING IN MALAWI | 第49-50页 |
| ·CONSTRAINTS OF PIG PRODUCTION IN MALAWI | 第50页 |
| ·IMPORTANCE OF LOCAL PIGS AMONG SMALLHOLDER FARMERS | 第50-56页 |
| ·CHEAP SOURCE OF PROTEIN(FOOD)IN ALMOST ALL PARTS OF MALAWI | 第50-53页 |
| ·DISEASE AND PARASITE RESISTANCE | 第53-54页 |
| ·RESEARCH PURPOSE | 第54-56页 |
| Chapter 3 Production and Development In Vitro of Porcine Embryos derived from ICSI | 第56-78页 |
| ·INTRODUCTION | 第56-59页 |
| ·MATERIALS | 第59-62页 |
| ·EQUIPMENT | 第59-60页 |
| ·REAGENTS | 第60-62页 |
| ·METHODS | 第62-66页 |
| ·OOCYTE COLLECTION AND IN VITRO MATURATION | 第62-63页 |
| ·IN VITRO FERLIZATION | 第63页 |
| ·ICSI | 第63-64页 |
| ·EMBRYO CULTURE | 第64页 |
| ·ISOLATION AND IN VITRO CULTURE OF NUCLEAR DONOR CELLS | 第64页 |
| ·ENUCLEATION OF RECIPIENT OOCYTES | 第64-65页 |
| ·NUCLEI TRANSFER PROCEDURES | 第65页 |
| ·REAL TIME-RT-PCR | 第65-66页 |
| ·EXPERIMENTAL DESIGN | 第66页 |
| ·STATISTICAL ANALYSIS | 第66-67页 |
| ·RESULTS | 第67-73页 |
| ·Maturation in vitro of porcine oocytes | 第67-70页 |
| ·Effect of 50 nM TSA on developmental competence of porcine ICSI embryos | 第70-71页 |
| ·Detection of Aurora-A mRNA expression during the first mitosis in early stage pig embryos | 第71-73页 |
| ·DISCUSSION | 第73-78页 |
| CONCLUSION | 第78-80页 |
| References | 第80-92页 |
| DEDICATION | 第92-94页 |
| ACKNOWLEDGEMENT | 第94页 |
