| SUMMARY | 第10-14页 |
| List of abbreviation | 第14-16页 |
| CHAPTER 1 LITERATURE REVIEW | 第16-52页 |
| 1.1 Soybean production and its constraints | 第16-32页 |
| 1.1.1 Soybean introduction | 第16-17页 |
| 1.1.2 Soybean production | 第17-18页 |
| 1.1.3 Abiotic constraints to production | 第18-32页 |
| 1.2 Traditional and modern plant breeding methods for crop improvement | 第32-47页 |
| 1.2.1 Plant breeding based on the selection of observed variants | 第32-33页 |
| 1.2.2 Plant breeding based on controlled mating | 第33-34页 |
| 1.2.3 Marker assisted breeding | 第34-47页 |
| 1.3 Background and objectives of the present study | 第47-52页 |
| CHAPTER 2 MATERIALS AND METHODS | 第52-60页 |
| 2.1 Procedure development for seed-flooding tolerance evaluation | 第52-55页 |
| 2.1.1 Plant materials | 第52页 |
| 2.1.2 The method for evaluation | 第52-55页 |
| 2.1.2.1 Evaluation of suitable seed-flooding duration | 第52-53页 |
| 2.1.2.2 Method to choose best media | 第53-54页 |
| 2.1.2.3 Methodology for choosing most effective indicators | 第54-55页 |
| 2.2 Molecular Mapping of QTL conferring seed-flooding tolerance | 第55-59页 |
| 2.2.1 Materials used in mapping the QTLs conferring seed-flooding tolerance | 第55页 |
| 2.2.2 Evaluation of phenotypic variation in seed flooding tolerance | 第55-57页 |
| 2.2.3 SNP calling and SNPLDB assembly | 第57页 |
| 2.2.4 Construction of the Joint linkage map and Joint Inclusive Composite Interval Mapping | 第57-58页 |
| 2.2.5 Mixed linear model-based genome wide association mapping | 第58-59页 |
| 2.2.6 Restricted two stage multi-locus-Genome wide association studies | 第59页 |
| 2.2.7 Candidate gene Prediction | 第59页 |
| 2.3 Statistical analysis | 第59-60页 |
| CHAPTER 3 ESTABLISHMENTOF EFFICIENT TESTINGPROCEDURES AND INDICATORSFOR SEED-FLOODING TOLERANCE IN SOYBEAN | 第60-66页 |
| 3.1 The suitable seed-flooding duration for tolerance evaluation | 第60-61页 |
| 3.2 The best method for seed-flooding tolerance evaluation | 第61-62页 |
| 3.3 The effective indicators for evaluation of seed-flooding tolerance | 第62-64页 |
| 3.4 Comparisons among the tested soybean accessions for their seed-flooding tolerance | 第64-65页 |
| 3.5 Summary of the Results | 第65-66页 |
| CHAPTER 4 QTL mapping and establishment of QTL-allele matrices for seed-flooding tolerance insoybean half-sib RILs | 第66-86页 |
| 4.1 Phenotypic variation of the relative seedling length and relative root length in LZM half-sib RILspopulation | 第66-68页 |
| 4.2 Population structure analysis | 第68-69页 |
| 4.3 Analysis of molecular variance for whole genome, RSL and RRL indicators | 第69页 |
| 4.4 QTL detection of RSL and RRL in LZM using JICIM | 第69-70页 |
| 4.5 QTL detection of RSL and RRL in LZM using MLM-GWAS procedure | 第70页 |
| 4.6 QTL mapping of RSL using RTM-GWAS | 第70-74页 |
| 4.6.1 Components of RSL QTL system in LZM | 第71页 |
| 4.6.2 QTLs detected in LZM for RSL | 第71-74页 |
| 4.7 QTL mapping of RRL using RTM-GWAS | 第74-76页 |
| 4.7.1 Components of RRL QTL system in LZM | 第74页 |
| 4.7.2 QTLs detected in LZM for RRL | 第74-76页 |
| 4.8 Comparisons among the results from JICIM, MLM-GWAS and RTM-GWAS | 第76-79页 |
| 4.9 Establishment of QTL-allele matrices of the three parents and their derived LZM population | 第79-84页 |
| 4.9.1 QTL-allele matrices of RSL | 第79-81页 |
| 4.9.2 QTL-allele matrices of RRL | 第81-84页 |
| 4.10 Summary of the Results | 第84-86页 |
| CHAPTER 5 PREDICTION OF POTENTIAL RECOMBINATIONS AMONG THREE PARENTSAND CANDIDATE GENES FOR SEED-FLOODING TOLERANCE | 第86-98页 |
| 5.1 Prediction of potential recombination from the parents and LZM QTL-allele structure | 第86-90页 |
| 5.1.1 Prediction of potential recombination of RSL from the parents and LZM QTL-allele structure | 第86-88页 |
| 5.1.2 Prediction of potential recombination for RRL from the parents and LZM QTL-allelestructure | 第88-90页 |
| 5.2 From QTL to gene system | 第90-97页 |
| 5.2.1 Gene system inferred from QTL system for RSL | 第90-93页 |
| 5.2.2 From QTL to candidate gene prediction of RRL indicator | 第93-97页 |
| 5.3 Summary of the Results- the candidate gene system conferring seed-flooding tolerance | 第97-98页 |
| CHAPTER 6 DISCUSSION | 第98-110页 |
| 6.1 Testing procedures for seed flooding tolerance | 第98-100页 |
| 6.2 Overlapped QTLs between two indicators and those in the literature | 第100页 |
| 6.3 QTL mapping and establishment of QTL-allele matrices for seed-flooding tolerance in soybeans | 第100-102页 |
| 6.4 Utilization of QTL-allele matrix in the improvement of seed-flooding | 第102-103页 |
| 6.5 Prediction of candidate genes for RSL and RRL | 第103-110页 |
| CHAPTER 7 MAJOR INNOVATION POINTS | 第110-112页 |
| 7.1 New methodology and indicator to screen seed-flooding tolerance resources | 第110页 |
| 7.2 RTM-GWAS is best method and identification of novel regions for seed-flooding tolerance | 第110页 |
| 7.3 QTL-allele and candidate gene system exploration of seed-flooding tolerance | 第110-112页 |
| REFERENCES | 第112-129页 |
| 附录 | 第129-138页 |
| ACKNOWLEDGEMENT | 第138-140页 |
| BIOGRAPHICAL DATA | 第140-142页 |
| DEDICATION | 第142页 |
