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中国湖北省草莓和番茄灰葡萄孢对二甲酰亚胺类和苯甲酰胺类杀菌剂的抗药性研究

ABSTRACT第8-9页
摘要第10-12页
List of Abbreviations第12-14页
CHAPTER 1. REVIEW OF LITERATURE第14-33页
    1.1. Botrytis cinerea - The causal agent of gray mold disease第14-17页
        1.1.1. Nomenclature and systematics第14-15页
        1.1.2. Biology, host range, and distribution第15页
        1.1.3. Adaptability and diversity第15-16页
        1.1.4. Epidemiology of B. cinerea第16-17页
    1.2. Gray mold diseases第17-22页
        1.2.1 Gray mold on strawberries第17-19页
            1.2.1.1. Symptoms第17-19页
            1.2.1.2. Disease cycle第19页
        1.2.2. Gray mold on tomatoes第19-22页
            1.2.2.1. Symptoms第19-22页
            1.2.2.2. Disease cycle第22页
    1.3. Management and Control第22-32页
        1.3.1 Integrated management第22-24页
        1.3.2. Chemical control of B. cinerea第24-25页
        1.3.3 Fungicide resistance of B. cinerea第25-32页
            1.3.3.1. Resistance profiles第25-28页
            1.3.3.2. Resistance Mechanism第28-29页
            1.3.3.3. Multi Drug Resistance第29页
            1.3.3.4. The risk of fungicide resistance development第29-30页
            1.3.3.5. Resistance management第30-32页
    1.4. Objectives of study第32-33页
CHAPTER 2. DETECTION AND MOLECULAR CHARACTERIZATION OF RESISTANCE TO THE PROCYMIDONE IN B. CINEREA FROM TOMATO AND STRAWBERRY IN HUBEI PROVINCE, CHINA第33-50页
    2.1. Introduction第33-34页
    2.2. Materials and methods第34-39页
        2.2.1. Fungicides第34页
        2.2.2. Source of B. cinerea isolates第34页
        2.2.3. Screening for resistance to fungicides第34-35页
        2.2.4. Sensitivity of B. cinerea mycelial growth to procymidone第35页
        2.2.5. Cross-resistance第35-36页
        2.2.6. Stability of resistant isolates第36页
        2.2.7. Fitness of resistant isolates第36-37页
        2.2.8. Efficacy of fungicides for control of B. cinerea第37页
        2.2.9. DNA extraction第37页
        2.2.10. Amplification and sequencing of Bc Os1 gene第37-39页
    2.3. Results第39-48页
        2.3.1. Frequency distribution of resistant isolates第39页
        2.3.2. Sensitivity of B. cinerea to procymidone第39-43页
        2.3.3. Cross-resistance第43-44页
        2.3.4. Stability of resistant isolates第44页
        2.3.5. Fitness of resistant isolates第44-46页
        2.3.6. Control efficacy for resistant isolates第46页
        2.3.7. Molecular characterization of Bc Os1第46-48页
    2.4. Discussion第48-50页
CHAPTER 3. CHARACTERIZATION OF ZOXAMIDE RESISTANCE IN BOTRYTIS CINEREA FROM TOMATO AND STRAWBERRY第50-69页
    3.1. Introduction第50-52页
    3.2. Materials and methods第52-57页
        3.2.1. Fungicides第52页
        3.2.2. Collection of B. cinerea isolates第52页
        3.2.3. Screening of B. cinerea isolates to zoxamide第52页
        3.2.4. Sensitivity of B. cinerea to zoxamide第52-53页
        3.2.5. Cross-resistance evaluation第53页
        3.2.6. Evaluation of stability for zoxamide resistance第53-54页
        3.2.7. Fitness of zoxamide resistant isolates第54页
        3.2.8. Evaluation of control efficacy for zoxamide resistant isolates第54-56页
        3.2.9. Amplification and sequencing of TUB2 gene第56-57页
    3.3. Results第57-66页
        3.3.1. Frequency distribution of resistant isolates第57页
        3.3.2. Sensitivity of B. cinerea to zoxamide第57-60页
        3.3.3. Cross-resistance of Zox~R isolates to carbendazim第60-61页
        3.3.4. Evaluation of resistance stability第61页
        3.3.5. Fitness of resistant isolates第61页
        3.3.6. Evaluation of control efficacy for Zox~R isolates第61-64页
        3.3.7. Molecular characterization of TUB2 gene第64-66页
    3.4. Discussion第66-69页
CHAPTER 4. SUMMARY AND PERSPECTIVES第69-71页
REFERENCES第71-94页
APPENDIX第94-102页
PUBLICATIONS第102-103页
ACKNOWLEDGMENT第103-105页

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