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An Analysis of The Hormones, Antioxidant Enzymes Activities under Drought Stress And Genomic-Wide Analysis of NAC Genes Family in Soybeans (Glycine max L.Merr.)

Abstract第6-7页
Abbreviations第8-10页
1. Literature review第10-19页
    1.1. Soybean (Glycine max (L.) Merr.)第10-11页
    1.2. Drought Stress第11页
    1.3. Reactive Oxygen Species (ROS)第11-13页
    1.4. Enzymatic Antioxidants第13-14页
    1.5. Plant Hormones and Drought Response第14-16页
        1.5.1. Abscisic acid (ABA)第15页
        1.5.2. Indole acetic acid (IAA)第15页
        1.5.3. Methyl jasmonate (JA)第15页
        1.5.4. Salicylic acid (SA)第15-16页
    1.6. Transcription Factor第16-19页
        1.6.1. NACs Transcription Factors第16-17页
        1.6.2. Structure of NACs第17页
        1.6.3. NACs Transcription Factors Functions and stress response第17-18页
        1.6.4. NACs Transcription Factors in Soybeans第18-19页
2. Objectives of the Study第19-20页
3. MATERIALS AND METHODS第20-29页
    3.1. Plant Material第20页
    3.2. Plant Growth Conditions and Drought Treatment第20页
    3.3. Determination of Antioxidant Enzyme Activity第20-22页
        3.3.1. Determination of SOD第20-21页
        3.3.2. Determination of APX第21-22页
        3.3.3. Determination of POD第22页
        3.3.4. Determination of CAT第22页
    3.4. Determination of Quantification of Lipid Peroxidation (Malon Di Aldehyde Levels)第22-23页
    3.5. Plant Hormones Protocols of IAA, GA, JA, SA and ABA第23页
    3.6. NAC Proteins Analysis in Soybeans第23-25页
        3.6.1. Identification of Soybean NAC Genes第23-24页
        3.6.2. Phylogenetic analysis第24页
        3.6.3. Chromosomal location, gene structure and Gene Duplication第24-25页
        3.6.4. Gene expression profile of out nine tissues第25页
    3.7. RNA Isolation第25-26页
    3.8. c DNA Synthesis第26页
    3.9. Quality test of synthesized cDNA第26-27页
    3.10. Quantitative Real-Time PCR and Statistical Analyses第27-28页
    3.11. Digital Gene-Expression Profiling第28页
    3.12. Statistical Analysis第28-29页
4. RESULTS第29-51页
    4.1. Physiological and Biochemical Parameters第29-33页
        4.1.1. Plant and drought treatment第29-30页
        4.1.2. Effect of Drought on Antioxidant Enzymes第30页
        4.1.3. Drought and lipid peroxidation第30-31页
        4.1.4. Effect of Drought on hormones levels第31-33页
    4.2. Wide comprehensive analysis, identification of NACs genes family and evaluate the transcriptomic changes during water deficit stress in Soybean第33-51页
        4.2.1. Identification of soybean NAC TFs and phylogenetic analysis of NAC genes in soybean, Arabidopsis and rice第33-36页
        4.2.2. Chromosomal location and gene duplication of Gm. NAC genes第36-39页
        4.2.3. Structural and motifs analysis of Gm. NAC genes第39-44页
        4.2.4. Expression profiles of Gm. NACs in different tissues through normal conditions第44-46页
        4.2.5. Clustering of DGEs with drought treatment第46-49页
        4.2.6. Expression analysis of Gm. NAC genes in response to drought第49-51页
5. Discussion第51-59页
    5.1. Physiological and biochemical parameters第51-55页
        5.1.1. Effects of Drought Stress on Lipid Peroxidation第51-52页
        5.1.2. Effects of Drought Stress on Antioxidant Enzymes Activities第52-54页
        5.1.3. Drought’s effects on plant hormones第54-55页
    5.2. Wide comprehensive analysis, identification of NACs genes family and evaluate the transcriptomic changes during water deficit stress in Soybean第55-59页
6. Reference第59-82页
7. Supplementary Tables第82-128页
ACKNOWLEDGEMENTS第128-129页

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