| 摘要 | 第6-7页 |
| abstract | 第7页 |
| CHAPTER Ⅰ INTRODUCTION AND LITERATURE REVIEW | 第15-39页 |
| 1.1 General background | 第15-16页 |
| 1.2 Plant's immune system | 第16-18页 |
| 1.3 Elicitors of Plant defense responses | 第18-21页 |
| 1.4 Induced plant resistance of a protective regime | 第21-23页 |
| 1.5 Type of elicitors | 第23-27页 |
| 1.5.1 Elicitors of abiotic | 第23页 |
| 1.5.2 Elicitors of biotic | 第23-27页 |
| 1.6 Pathogenesis-related(PR)proteins | 第27-28页 |
| 1.7 Recombinant protein expression | 第28-30页 |
| 1.8 Tomato plant defense response | 第30-31页 |
| 1.9 Abiotic and biotic stresses-related pathways | 第31-36页 |
| 1.9.1 Abscisic acid(ABA)signaling pathway | 第32-33页 |
| 1.9.2 Production of reactive oxygen species(ROS) | 第33-34页 |
| 1.9.3 Jasmonic Acid(JA)signaling pathway | 第34页 |
| 1.9.4 Salicylic acid signaling pathway | 第34-35页 |
| 1.9.5 Nitric oxide(NO)signaling in plant defense | 第35-36页 |
| 1.10 Hrip1 history | 第36-37页 |
| 1.11 Signification of study | 第37页 |
| 1.12 Research aim and purpose | 第37-39页 |
| CHAPTER Ⅱ MATERIALS AND METHODS | 第39-55页 |
| 2.1 Materials and methods | 第39-40页 |
| 2.1.1 Plants,Agrobacterium strains | 第39页 |
| 2.1.2 Reagents and instruments | 第39-40页 |
| 2.1.3 Buffer preparation | 第40页 |
| 2.2 Experimental methods | 第40-45页 |
| 2.2.1 Preparation of YPD medium of solid and liquid | 第40页 |
| 2.2.2 Screening yeast cloning of Hrip | 第40-41页 |
| 2.2.3 Eukaryotic expression of Hrip | 第41页 |
| 2.2.4 Purification of Hrip | 第41-42页 |
| 2.2.5 Assay of protein concentration | 第42-44页 |
| 2.2.6 Confirmation of Hrip1 by SDS-PAGE | 第44-45页 |
| 2.2.7 Identification of a molecular weight | 第45页 |
| 2.3 Cultivar condition of tomato | 第45-46页 |
| 2.4 Activities of induced HR | 第46页 |
| 2.5 Extraction of total RNA | 第46-49页 |
| 2.5.1 cDNA synthesis for RT-qPCR of gene expression | 第47页 |
| 2.5.2 RT-qPCR of gene expression | 第47-49页 |
| 2.6 Detection of ROS accumulation | 第49页 |
| 2.7 Hrip1-induced disease resistance in tomato plant | 第49-50页 |
| 2.8 Analysis of TYLCV concentration | 第50-52页 |
| 2.8.1 Total DNA extraction for TYLCV | 第50-51页 |
| 2.8.2 Detection of TYLCV concentration by RT-qPCR | 第51-52页 |
| 2.8.3 Determination of plasmid copy number and cultural condition | 第52页 |
| 2.9 Confirmation of positive control | 第52-55页 |
| 2.9.1 Preparation of LB medium of solid and liquid | 第52页 |
| 2.9.2 Plasmid extraction | 第52-53页 |
| 2.9.3 Detection of TYLCV’s DNA to confirm positive control | 第53-55页 |
| CHAPTER Ⅲ RESULTS AND DISCUSSION | 第55-66页 |
| 3.1 Results | 第55-64页 |
| 3.1.1 Purification of recombinant protein and induced HR | 第55-56页 |
| 3.1.2 Accumulation of ROS | 第56-57页 |
| 3.1.3 Hrip1 caused expression of defense-related gene | 第57-59页 |
| 3.1.4 Confirmation of positive control | 第59-60页 |
| 3.1.5 Detection of tomato-infected TYLCV | 第60-61页 |
| 3.1.6 Hrip1 induced disease resistance in tomato | 第61-63页 |
| 3.1.7 Hrip1-reduced level of concentration of TYLCV-infected tomato | 第63-64页 |
| 3.2 Discussion | 第64-66页 |
| CONCLUSIONS | 第66-67页 |
| REFERENCES | 第67-76页 |
| APPENDIX | 第76-77页 |
| TECHNICAL ROUT MAP | 第76-77页 |
| ACKNOWLEDGEMENTS | 第77-78页 |
| BIOGRAPHY | 第78-84页 |
