| 摘要 | 第6-7页 |
| Abstract | 第7-8页 |
| Chapter I Introduction | 第15-33页 |
| 1.1 Signals triggering leaf senescence | 第17-27页 |
| 1.1.1 Age dependent leaf senescence | 第18页 |
| 1.1.2 Sugars | 第18-19页 |
| 1.1.3 Hormonal regulation of leaf senescence | 第19-26页 |
| 1.1.3.1 Cytokinins | 第19-21页 |
| 1.1.3.2 Auxin | 第21-22页 |
| 1.1.3.3 Ethylene | 第22-23页 |
| 1.1.3.4 Salicylic Acid | 第23-24页 |
| 1.1.3.5 Jasmonic Acid | 第24-25页 |
| 1.1.3.6 Abscisic Acid | 第25-26页 |
| 1.1.4 Environmental factors | 第26-27页 |
| 1.2 Genetic regulation of leaf senescence | 第27-33页 |
| 1.2.1 Senescence regulation: role of transcription factors | 第28-29页 |
| 1.2.1.1 WRKY transcription factors | 第28页 |
| 1.2.1.2 NAC family transcription factors | 第28-29页 |
| 1.2.1.3 Other TF | 第29页 |
| 1.2.2 Signal perception and transduction: senescence regulation by kinases | 第29-33页 |
| 1.2.2.1 MAPK signaling during leaf senescence | 第30页 |
| 1.2.2.2 Receptor like kinases (RLKS) 17 | 第30-31页 |
| 1.2.2.3 Leucine rich repeat receptor like kinases (LRR-RLKs) 18 | 第31-33页 |
| Chapter II Screening of Arabidopsis TDNA lines (LRR-RLKs) for senescence mutants | 第33-51页 |
| 2.1 Introduction | 第33-34页 |
| 2.2 Materials and Methods | 第34-37页 |
| 2.2.1 Plant growth conditions | 第34页 |
| 2.2.2 Arabidopsis T-DNA insertion mutants | 第34-35页 |
| 2.2.3 Plant genomic DNA extraction | 第35页 |
| 2.2.4 Identification Verification and isolation of Arabidopsis Homozygous T-DNA insertion lines | 第35-37页 |
| 2.2.5 Phenotypic screening of Homozygous T-DNA mutants for leaf senescence | 第37页 |
| 2.3 Results | 第37-46页 |
| 2.3.1 screening of insertion mutants | 第37-38页 |
| 2.3.2 Genotyping of T-DNA lines | 第38-41页 |
| 2.3.3 Screening of LRR-RLKs mutants based on phenotypic expression | 第41-46页 |
| 2.3.3.1 Expression profiles of senescence regulating receptor like kinase (LRR-RLKs)during leaf senescence | 第43-46页 |
| 2.4 Discussion | 第46-51页 |
| 2.4.1 Reverse genetics and use of T-DNA mutagenesis | 第46-47页 |
| 2.4.2 Identification of homozygous T-DNA lines | 第47-48页 |
| 2.4.3 Knockout mutant analysis of LRR-RLK mutants with respect to their involvement in leaf senescence | 第48-49页 |
| 2.4.4 Role of Senescence regulating receptor like kinases (SRKs) during leaf senescence | 第49-51页 |
| Chapter III Genome wide identification of LRR-RLKs gene family from Nicotiana tabacum | 第51-72页 |
| 3.1 Introduction | 第51-52页 |
| 3.2 Materials and Methods | 第52-53页 |
| 3.2.1 Identification of LRR-RLKs gene family from Nicotiana tabacum | 第52页 |
| 3.2.2 Prediction of transmembrane helices | 第52页 |
| 3.2.3 Multiple sequence alignment and Phylogenetic analysis of LRR-RLKs genefamily from Nicotiana tabacum | 第52-53页 |
| 3.3 Results | 第53-69页 |
| 3.3.1 Identification of LRR-RLKs gene family from Nicotiana tabacum | 第53-64页 |
| 3.3.2 Prediction of transmembrane helices | 第64-65页 |
| 3.3.3 Multiple sequence alignment and Phylogenetic analysis of LRR-RLKs genefamily from Nicotiana tabacum | 第65-69页 |
| 3.4 Discussion | 第69-72页 |
| Chapter IV Conclusion | 第72-74页 |
| References | 第74-90页 |
