| Abstract (in Chinese) | 第1-8页 |
| Abstract (in English) | 第8-10页 |
| Abbreviations | 第10-11页 |
| 1. The inhibition of phosphoinositide signaling pathway affects the pollen tube development in Picea wilsonii by changing the cytoplasmic Ca~(2+) gradient and deposition of cell wall components | 第11-38页 |
| ·Introduction | 第11-14页 |
| ·Materials and methods | 第14-16页 |
| ·Plant materials and pollen tube growth conditions | 第14页 |
| ·Pollen tube growth determination and morphological observation | 第14页 |
| ·Confocal laser scanning microscopy (CLSM) imaging | 第14-15页 |
| ·FTIR microspectroscopy | 第15页 |
| ·Aniline blue staining of callose | 第15页 |
| ·Immunolabelling of pectins and arabinogalactan proteins | 第15-16页 |
| ·Results | 第16-26页 |
| ·Pollen tube growth and morphology | 第16-19页 |
| ·Intracellular Ca~(2+) distribution | 第19页 |
| ·FTIR microspectroscopy | 第19-22页 |
| ·Callose deposition in pollen tube walls | 第22页 |
| ·Pectin and arabinogalactan-protein distribution in pollen tube | 第22-26页 |
| ·Discussion | 第26-31页 |
| ·References | 第31-38页 |
| 2.A natomical and chemical characteristics of foliar vascular bundles in four 1eed ecotypes adapted to different habitats | 第38-77页 |
| ·Introduction | 第38-41页 |
| ·Materials and methods | 第41-45页 |
| ·Plant material and sampling sites | 第41-42页 |
| ·Isolation of foliar vascular bundles and cell walls of bundle sheath cells | 第42页 |
| ·Field emission scanning electron microscopy (FE-SEM) | 第42页 |
| ·Light microscopy and histology | 第42-43页 |
| ·Fourier transform infrared (FTIR) microspectroscopy analysis | 第43页 |
| ·Extraction of cell wall-binding proteins and assay of their expression | 第43-44页 |
| ·Two-dimensional electrophoresis (2-DE) and image analysis | 第44-45页 |
| ·Results | 第45-61页 |
| ·Habitat conditions, leaf water status and stress responses | 第45-46页 |
| ·Anatomical characteristics of foliar vascular bundles | 第46-49页 |
| ·Anatomical characteristics of the cell walls of foliar bundle sheath | 第49页 |
| ·FTIR analysis of the cell walls of bundle tissues | 第49-54页 |
| ·Histochemical analysis of the cell walls of bundle tissues | 第54-57页 |
| ·Contents of cell wall-binding proteins and their different expression | 第57-61页 |
| ·Discussion | 第61-69页 |
| ·Anatomical characteristics of foliar vascular bundles | 第61-63页 |
| ·Anatomical properties of the cell walls of vascular bundle sheath | 第63-64页 |
| ·Chemical characteristics of the cell walls of foliar vascular bundles | 第64-65页 |
| ·Histochemical characteristics of the cell walls of foliar vascular bundles | 第65-67页 |
| ·Contents of cell wall-binding proteins and their expression | 第67-69页 |
| ·Conclusions | 第69页 |
| ·References | 第69-77页 |
| Acknowledgements | 第77-78页 |
| Resume | 第78-79页 |
| Publications | 第79-81页 |
