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帕金森氏病体外细胞模型的建立及胞二磷胆碱对多巴胺能神经元保护作用的研究

Abbreviations第7-9页
1 Introduction第9-13页
2 Literature review第13-59页
    2.1 Parkinson's disease第13-32页
    2.2 Models of PD Research第32-44页
        2.2.1 Models of PD in vivo第32-36页
        2.2.2 Models of PD in vitro第36-44页
    2.3 Citicoline第44-59页
        2.3.1 Chemical character and metoblism第44-46页
        2.3.2 Pharmacokinetics第46-48页
        2.3.3 Pharmacodynamics on CNS第48-55页
        2.3.4 Mechanism of neuroprotective effect of citicoline第55-59页
3 Materials and methods第59-71页
    3.1 Materials and devices第59页
    3.2 Methods第59-71页
        3.2.1 Mice mesencephalic primary culture第59-64页
        3.2.2 Human neuroblastoma cell line第64-65页
        3.2.3 TH-immunostaining第65-67页
        3.2.4 Identification of the apoptosis第67-68页
        3.2.5 LDH release第68页
        3.2.6 MTT assay第68-69页
        3.2.7 Live/dead cell viability and assay of cytotoxicity第69页
        3.2.8 Cell death identified by propidium iodide (PI) uptake第69-70页
        3.2.9 Mitochordrial potential第70页
        3.2.10 Statistical analysis第70-71页
4 Results第71-101页
    4.1 Cell models of degeneration of dopaminergic neurons in vitro第71-86页
        4.1.1 The degeneration of dopaminergic neurons induced with MPP~+第71-79页
        4.1.2 The degeneration of dopaminergic neurons induced with glutamate第79-83页
        4.1.3 The toxicity of MPP~+ in human neuroblastoma cell line第83-86页
    4.2 The neuroprotective effects of citicoline第86-101页
        4.2.1 The effect of citicoline on the degeneration of dopaminergic neurons in normal culture condition第86-87页
        4.2.2 Citicoline protected dopaminergic neurons from death induced with MPP~+第87-98页
        4.2.3 The effect of citicoline on the degeneration of dopaminergic neurons indued with glutamate第98-101页
5 Discussion第101-116页
    5.1 The neurotoxicity of MPP~+ on dopaminergic neurons in mice mesencephalic dissociated culture第101-104页
    5.2 The neurotoxicity of MPP~+ in STA-NB-3 human neuroblastoma cell line第104-106页
    5.3 Two different cell death models induced with MPP~+ and glutamate第106-110页
    5.4 The neuroprotective effect of citicoline on protecting dopaminergic neurons from death induced with MPP~+第110-113页
    5.5 The neuroprotective effect of citicoline on preventing dopaminergic neurons from degeneration induced with glutamate第113-116页
6 Conclusion第116-117页
7 References第117-140页
作者简介第140-141页
中文摘要第141-147页
英文摘要第147页
原创性声明第153页

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