| GLOSSARY OF ABBREVIATIONS | 第1-9页 |
| ABSTRACT | 第9-16页 |
| Part Ⅰ Evaluation Of The Diagnostic Test | 第16-29页 |
| 1 Instruction | 第16-17页 |
| 2 Material and methods | 第17-22页 |
| ·Sources of general laboratory consumable | 第17页 |
| ·Procedure | 第17-22页 |
| ·Blood Samples | 第17-18页 |
| ·Separation of peripheral blood mononuclear cells (PBMNs) | 第18页 |
| ·RNA Isolation | 第18-19页 |
| ·cDNA Synthesis | 第19-20页 |
| ·Primer/probe design | 第20页 |
| ·Taqman PCR condition | 第20-21页 |
| ·Spiking experiment using LC-5 Cells | 第21页 |
| ·Theoretical basis of FQ-PCR | 第21-22页 |
| 3 Results | 第22-25页 |
| ·Evaluation of accuracy | 第22-23页 |
| ·Assessment of reproducibility | 第23-24页 |
| ·Standard Curve and Amplification Plots (Fi93 and Fig.4) | 第24页 |
| ·The results of clinical samples | 第24-25页 |
| 4 Discussions | 第25-29页 |
| ·The marker | 第25-26页 |
| ·The method | 第26-29页 |
| PartⅡ Observation Of CTCs In Patients With Non-Small Cell Lung Cancer In Peroperative Period | 第29-49页 |
| 1 Introduction | 第29-30页 |
| 2 Material and method | 第30-33页 |
| ·Clinical data | 第30-32页 |
| ·Reagents and equipments (See part 1) | 第32页 |
| ·Procedure | 第32-33页 |
| ·Statistical Analysis | 第33页 |
| 3 Results | 第33-40页 |
| ·The level of CK19 m RNA in PB during peroperative period | 第33-34页 |
| ·The correlation between CK19 m RNA in PB and classic clinicopathological parameters | 第34-36页 |
| ·Evaluation of Diagnostic Test of CEA | 第36-37页 |
| ·The level of CEA m RNA in PB during perioperative period | 第37-38页 |
| ·The correlation between CEA m RNA in PB and conventional clinicopathological feature | 第38-40页 |
| ·The preoperative positive rate of CK19/CEA | 第40页 |
| 4 Discussions | 第40-48页 |
| ·Clinicopathological correlation | 第41-43页 |
| ·The effect of the sequence of vessel ligation (SOVL) | 第43-44页 |
| ·The creativity of the study | 第44-45页 |
| ·The prospective direction and near future working | 第45-48页 |
| 5 Conclusions | 第48-49页 |
| Part Ⅲ Fluctuation Of CTCs In Lung Cancer Patients Before And After Radioterapy | 第49-55页 |
| 1 Introduction | 第49页 |
| 2 Materials and methods | 第49-51页 |
| 3 Results | 第51-53页 |
| ·Comparison of CK19 m RNA in PB before and after radiotherapy | 第51页 |
| ·The relationship between CK19 m RNA in PB and classic clinicopathological parameter | 第51-53页 |
| 4 Discussions | 第53-55页 |
| PART Ⅳ Comparison Of CTCs In Patients With Lung Carcinoma Before And After Chemotherapy | 第55-66页 |
| 1 Instruction | 第55页 |
| 2 Material and Methods | 第55-58页 |
| 3 Results | 第58-60页 |
| ·Comparison of CK19 m RNA in PB before and after chemotherapy | 第58页 |
| ·Comparison of md11 m RNA in PB before and after chemotherapy | 第58页 |
| ·The relationship between CK19 m RNA in PB and classic linocopathological feature | 第58-59页 |
| ·The correlation between the change of CK19 m RNA and mdrl mRNA before and after chemotherapy | 第59-60页 |
| ·The comparison of md11 transcription in PBL of patients with SCLC or NSCLC during the procedure | 第60页 |
| ·The positive rate of CK19 m RNA before and after chemotherapy | 第60页 |
| 4 Discussions | 第60-66页 |
| ·Dynamic monitoring of the CTCs in the procedure of treatment and its clinical value | 第60-62页 |
| ·The clinical significance of longitudinal studying of the expression level of md11 in PBL during chemotherapy | 第62-66页 |
| Part Ⅴ Observation Of CTCs Based On Postoperative Chemotherapy In Patients With Non-Small Cell Lung Cancer | 第66-73页 |
| 1 Introduction | 第66页 |
| 2 Materials and Methods | 第66-68页 |
| 3 Results | 第68-69页 |
| ·The changing tendency of CK19 m RNA in PB at each time point during postoperative chemotherapy | 第68页 |
| ·Comparison of positivity during postoperative chemotherapy | 第68-69页 |
| ·The expression level of CK19 m RNA in PB of patients with/Without clinical relapse/metastasis | 第69页 |
| 4 Discussions | 第69-73页 |
| REFERENCES | 第73-79页 |
| SUMMARY | 第79-80页 |
| ACKNOWLEDGEMENTS | 第80-177页 |
