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杏鲍菇、平菇及白玉菇碱提多糖的分离纯化及结构分析

Abstract第4-5页
摘要第6-9页
List of abbreviations第9-10页
CHAPTER 1 INTRODUCTION第10-30页
    1. Background on Edible Mushrooms第10-11页
    2. Chemical Composition of Edible Mushrooms第11-12页
    3. Structural Composition of Mushroom Polysaccharides第12-17页
    4. Bioactivities of polysaccharides and their complexes第17-19页
    5. General Procedures for Extraction, Isolation and Purification of Polysaccharides第19-21页
        5.1 Extraction of polysaccharides from mushrooms第20页
        5.2 Isolation and purification of polysaccharides from mushrooms第20-21页
    6. Structural Elucidation of Polysaccharide from Mushroom第21-25页
        6.1 Monosaccharide composition determination第22页
        6.2 Molecular weight determination第22页
        6.3 FT-IR spectroscopy第22-23页
        6.4 Methylation analysis by GC-MS第23-24页
        6.5 NMR spectroscopy第24-25页
    7. Background on Selected Edible Mushrooms第25-28页
        7.1 Pleurotus eryngii第25-26页
        7.2 Pleurotus ostreatus第26-27页
        7.3 Hypsizigus marmoreus第27-28页
    8. Research Interests and Contents第28-30页
        8.1 Research motives and interest第28-29页
        8.2 Research objectives第29-30页
CHAPTER 2 MATERIALS AND METHODS第30-37页
    1. Biological Materials, Chemicals and Reagents第30-32页
        1.1 Biological materials第30页
        1.2 Chemicals and r6eagents第30-31页
        1.3 Equipments第31-32页
    2. Experimental Methods第32-37页
        2.1 Extraction of crude polysaccharides第32页
        2.2 Isolation of water-soluble polysaccharides第32-33页
        2.3 Fractionation of crude polysaccharides第33页
        2.4 Analysis of physico-chemical characteristics of polysaccharides第33-34页
        2.5 Structural characterization第34-37页
CHAPTER 3 RESULTS AND DISCUSSION第37-56页
    1. Extraction Yield of Crude Polysaccharides第37-38页
    2. Isolation of Water-Soluble Polysaccharides第38-42页
        2.1 Total carbohydrate content第39页
        2.2 Uronic acids content第39-40页
        2.3 Protein content第40-41页
        2.4 Monosaccharide composition analysis第41-42页
    3. Fractionation of polysaccharides on sepharose CL-6B column第42-45页
        3.1 APEP-S gel permeation chromatography第42-43页
        3.2 APOP-S gel permeation chromatography第43-44页
        3.3 AHMP-S gel permeation chromatography第44-45页
    4. Purification of polysaccharides on sephadex G-50 column第45-48页
        4.1 APEP-S-II gel-permeation chromatography第45-46页
        4.2 APOP-S-II gel-permeation chromatography第46-47页
        4.3 AHMP-S-II gel-permeation chromatography第47-48页
    5. Structural Analysis第48-56页
        5.1 Homogeneity and molecular weight distribution第48-49页
        5.2 FT-IR spectrum analysis第49-51页
        5.3 ~(13)C NMR analysis第51-56页
Conclusion第56-57页
References第57-69页
Acknowledgement第69页

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