| Abstract | 第7-8页 |
| 摘要 | 第9-11页 |
| Chapter 1 LITERATURE REVIEW | 第11-33页 |
| 1.1 Background | 第11-12页 |
| 1.2 Triazole Fungicide | 第12-15页 |
| 1.2.1 Triadimefon | 第13-15页 |
| 1.2.2 Ecotoxicity of triadimefon | 第15页 |
| 1.3 Chiral resolution of triazole pesticides | 第15-20页 |
| 1.3.1 Chiral HPLC separation of triazole pesticides | 第16-19页 |
| 1.3.2 The chiral CE separation of triazole pesticides | 第19页 |
| 1.3.3 Chiral SFC and GC separation of triazole pesticides | 第19-20页 |
| 1.4 Study on environmental behavior of triazole pesticides | 第20-22页 |
| 1.4.1 Half-life study | 第20-21页 |
| 1.4.2 Study on the selective degradation of enantiomers | 第21-22页 |
| 1.4.3 Photochemical and optical physics research and others | 第22页 |
| 1.5 Soil micro-flora | 第22-28页 |
| 1.5.1 Distribution of the microbial communities in the surface and subsurface soil | 第24-26页 |
| 1.5.2 The bacterial communities in the soil | 第26-28页 |
| 1.6 Measuring the impact of the residual pesticides on the different microbial communities in the soil | 第28-32页 |
| 1.6.1 Metagenomics | 第29-30页 |
| 1.6.2 DNA sequences analysis | 第30-32页 |
| 1.7 Objectives and significances of the study | 第32-33页 |
| 1.7.1 The main objectives of this study | 第32页 |
| 1.7.2 The significances of this study | 第32-33页 |
| Chapter 2 Materials and methods | 第33-40页 |
| 2.1 Materials | 第33-34页 |
| 2.1.1 Chemicals and reagents | 第33页 |
| 2.1.2 Soils collection and preparation | 第33-34页 |
| 2.2 Methods | 第34-40页 |
| 2.2.1 Experimental design | 第34页 |
| 2.2.2 Enantiomerization and degradation method | 第34-36页 |
| 2.2.3 High throughput sequences technology | 第36-40页 |
| Chapter 3 The impact of the chiral triadimefon compounds on bacterial communities in different types of soils | 第40-53页 |
| 3.1 Introduction | 第40-41页 |
| 3.2 Material and Method | 第41-42页 |
| 3.2.1 Chemical and soils collection and preparation | 第41页 |
| 3.2.2 Experimental design | 第41-42页 |
| 3.2.3 High throughput sequences technology | 第42页 |
| 3.3 Results and discussion | 第42-50页 |
| 3.3.1 Sequencing and bacterial diversity indices | 第42-45页 |
| 3.3.2 Microbial community comparisons | 第45-46页 |
| 3.3.3 OTU distribution under different TF compounds treatments | 第46-47页 |
| 3.3.4 Bacteria community structure in blank soils | 第47-49页 |
| 3.3.5 Bacteria community structure in TFs contaminated soils | 第49-50页 |
| 3.4 The relationship between microbial communities and environmental Parameters | 第50-52页 |
| 3.5 Conclusions | 第52-53页 |
| Chapter 4 The influence of microbial communities for triadimefon enantiomerization in Soils | 第53-64页 |
| 4.1 Introduction | 第53-54页 |
| 4.2 Material and Methods | 第54-55页 |
| 4.2.1 Chemical and soils collection and preparation | 第54页 |
| 4.2.2 Experimental design | 第54-55页 |
| 4.2.3 Extraction methods | 第55页 |
| 4.2.4 Analytical procedure | 第55页 |
| 4.2.5 Statistical analysis | 第55页 |
| 4.2.6 High-throughput sequencing | 第55页 |
| 4.3 Results and discussion | 第55-63页 |
| 4.3.1 Enantiomerization of Triadimefon in different soils | 第55-56页 |
| 4.3.2 OTUs and Bacterial Community Species Diversity | 第56-58页 |
| 4.3.3 Variation in Bacterial Community Composition | 第58-60页 |
| 4.3.4 Correlations between major bacterial groups and triadimefon enantiomerization | 第60-62页 |
| 4.3.5 Correlations between Major Bacterial Groups and Environmental Factors | 第62-63页 |
| 4.4 Conclusion | 第63-64页 |
| Chapter 5 The influence of microbial communities on the degradation of triadimefon racemate compound in soils | 第64-76页 |
| 5.1 Introduction | 第64-65页 |
| 5.2 Material and methods | 第65-66页 |
| 5.2.1 Chemical and soils collection and preparation | 第65页 |
| 5.2.2 Experimental design | 第65页 |
| 5.2.3 Extraction methods | 第65页 |
| 5.2.4 Analytical procedure | 第65页 |
| 5.2.5 Statistical analysis | 第65页 |
| 5.2.6 High-throughput sequencing | 第65-66页 |
| 5.3 Results and discussion | 第66-75页 |
| 5.3.1 Enantioselective and Kinetics Degradation of racemic triadimefon in different soils | 第66-68页 |
| 5.3.2 Richness and diversity of microbial communities | 第68-69页 |
| 5.3.3 Variation in bacterial community composition | 第69-71页 |
| 5.3.4 OTU distribution under different TF compounds treatments | 第71-72页 |
| 5.3.5 Certain bacterial taxa related to TF-Rac degradation | 第72-74页 |
| 5.3.6 Correlations between major bacterial groups and environmental factors | 第74-75页 |
| 5.4 Conclusion | 第75-76页 |
| Chapter 6 Summary and perspective work | 第76-77页 |
| References | 第77-99页 |
| Funding supports | 第99-100页 |
| Appendices | 第100-103页 |
| Acknowledgement | 第103-104页 |
